Control of mRNA stability and translation by G3BP RNA-binding proteins

G3BP RNA 结合蛋白控制 mRNA 稳定性和翻译

• 批准号: RGPIN-2018-06485
• 负责人: Smibert, Craig
• 金额: $ 2.62万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=665421
• 关键词: Control; mRNA; stability; translation; G3BP

The growth and development of all multi-celled organisms—including humans—requires that cells within the body adopt specialized functions. Cell specialization involves the presence of different proteins in different cell types (e.g. muscle proteins in muscle cells, liver proteins in liver cells, etc.). Cells produce individual proteins using specific signals known as mRNAs in a process called translation. We are studying two strategies that are employed to control which proteins are present in any particular cell. In one strategy, translation of specific proteins within a cell can be turned on or off. In the other strategy, the production of a protein can be blocked by destroying the corresponding mRNA via mRNA degradation. The decision to translate or degrade an mRNA is controlled by proteins—called RNA-binding proteins (RBPs)—that bind mRNAs. The work in this proposal is aimed at understanding how RBPs stabilize mRNAs and turn on their translation. These types of mechanisms function throughout the life span of all multi-celled organisms and play particularly prominent roles during the earliest stages of development. We are using a simple organism—the fruit fly—to study how RBPs are controlled during the earliest stages of the organism's life. Since there are many similarities between how flies and humans grow and develop, our work is applicable to humans.

所有多细胞生物体（包括人类）的生长和发育都要求体内细胞具有专门的功能，细胞特化涉及不同细胞类型中存在不同的蛋白质（例如肌肉细胞中的肌肉蛋白、肝细胞中的肝蛋白、细胞在称为翻译的过程中使用称为 mRNA 的特定信号产生单个蛋白质。其中一种策略是细胞内特定蛋白质的翻译。可以打开或关闭。另一种策略是，通过 mRNA 降解破坏相应的 mRNA，从而阻断蛋白质的产生。翻译或降解 mRNA 的决定是由与 mRNA 结合的蛋白质（称为 RNA 结合蛋白 (RBP)）控制的。该提案旨在了解 RBP 如何稳定 mRNA 并开启其翻译，这些机制在所有多细胞生物的整个生命周期中发挥作用，并在发育的最早阶段发挥特别重要的作用——果蝇——研究 RBP 的作用由于果蝇和人类的生长和发育有许多相似之处，因此我们的工作适用于人类。