Indction of odontoblasts from cranial neural crest stem cells

颅神经嵴干细胞诱导成牙本质细胞

基本信息

批准号：
15591931
负责人：
YAMAZAKI Hidetoshi
金额：
$ 2.24万
依托单位：
National University Corporation Tottori University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

Murine neural crest(NC) cells are generated from dorsal tip of neural folds at E 9.0. NC cells are divided into two populations ; cranial neural crest(CNC) and trunk neural crest(TNC). CNC can differentiate into several cell lineages containing osteoblasts, chondrocytes and odontoblasts. Furthermore, it is known that CNC participate in organogenesis such as cranioface containing jaws and teeth, and thymus. However, characteristics of these NC cells have not been clarified. Therefore, we assessed the presence, distribution and potential for differentiation of these NC cells. In addition, we tried to make a system to detect the differentiation of CNC into odontoblasts by using GFP.We show results of our research as follows :1)We used P0-Cre mice, which express Cre gene in the neural crest cells, and we can trace neural crest-derived cells as LacZ-expressing cells. Using this system, we clarified the presence, distribution and potential for differentiation of NC in the thymus. Furthermore, we assessed the characteristics of NC cells by flow cytometry.2)Next, using these systems, we clarified that about 70% mesenchymal cells of tooth buds were composed of NC-derived cells, and large numbers of these NC cells in the tooth buds expressed PFDGFRα, integrin αV, CD44.3)We found that NC-derived cells in the tooth buds could differentiate into not only odontoblasts but also chondrocytes and osteoblasts.4)We made three lines of transgenic mice carrying GFP under the control of Dentin sialophosphoprotein(DSPP). However, we didn't get transgenic mice that express GFP in odontoblasts.

小鼠神经嵴（NC）细胞从E 9.0的神经皱襞背端产生，NC细胞分为两个群体：颅神经嵴（CNC）和躯干神经嵴（TNC）可以分化成含有成骨细胞的多种细胞谱系。此外，已知 CNC 参与器官发生，例如包含颌骨和牙齿的颅面以及胸腺。 NC 细胞尚未阐明。因此，我们评估了这些 NC 细胞的存在、分布和分化潜力。此外，我们尝试制作一个使用 GFP 检测 CNC 向成牙本质细胞分化的系统。我们展示了我们的结果。研究如下：1)我们使用P0-Cre小鼠，在神经嵴细胞中表达Cre基因，我们可以追踪神经嵴衍生细胞作为LacZ表达细胞，利用该系统，我们阐明了其存在、分布和潜力。为了此外，我们通过流式细胞术评估了NC细胞的特征。2)接下来，使用这些系统，我们澄清了牙芽中约70%的间充质细胞由NC衍生细胞组成，并且数量很大。牙芽中的这些 NC 细胞表达 PFDGFRα、整合素 αV、CD44。3）我们发现牙芽中的 NC 衍生细胞不仅可以分化为成牙本质细胞4)我们制作了三个在牙本质唾液酸磷蛋白(DSPP)控制下携带GFP的转基因小鼠，但我们没有获得在成牙本质细胞中表达GFP的转基因小鼠。