Hard tissue regeneration using dental mesenchymal stem cells and embryonic stem cells that enabled us to trace neural crest cells and mesodermal cells

使用牙科间充质干细胞和胚胎干细胞进行硬组织再生，使我们能够追踪神经嵴细胞和中胚层细胞

• 批准号: 21390489
• 负责人: YAMAZAKI Hidetoshi
• 金额: $ 11.56万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390489/
• 关键词: 口腔解剖学(含組織学・発生学); 神経堤細胞; 間葉細胞; 象牙芽細胞; 胚性幹細胞; Dspp遺伝子; 中胚葉; 歯髄間葉細胞; 骨芽細胞; 中胚葉由来細胞; 色素細胞; 間葉系幹細胞; 歯髄; 硬組織; 再生

Using transgenic mice that enabled us to trace NC cells and mesodermal cells as YFP^+ cells, respectively, we established ES cell lines to investigate whether NC cells or mesodemal cells were generated from ESCs in the culture. We found that NC-derived melanocytes were efficiently generated from NC-derived YFP^+ cells. The NC-derived YFP^+ cells also expressed strongly NC-associated genes. We also found that NC-derived YFP^+ cells differentiated into not only melanocytes, but also neurons, adipocytes, and osteoblasts. Furthermore, to detect the differentiation of odontoblasts in vivo and in vitro, we made Dspp Knock-In ES (Dspp-KI ES) cell lines by targeting vector that inserted GFP into Dspp gene. Then, Dspp-KI ES cells were injected into blastocysts, and large numbers of chimera mice were obtained. Dspp-KI ES-derived cells cultured for 6 days also were transplanted into kidney capsule, and 4 weeks after YFP^+ cells were detected in the transplanted tissues. We further try to differentiate these ESCs into odontoblasts in vitro through NC.

使用转基因小鼠，我们能够分别追踪作为 YFP^+ 细胞的 NC 细胞和中胚层细胞，我们建立了 ES 细胞系，以研究 NC 细胞或中胚层细胞是否由培养物中的 ESC 产生。我们发现 NC 衍生的黑素细胞是从 NC 衍生的 YFP^+ 细胞有效生成的。 NC 衍生的 YFP^+ 细胞也强烈表达 NC 相关基因。我们还发现NC衍生的YFP^+细胞不仅分化为黑素细胞，还分化为神经元、脂肪细胞和成骨细胞。此外，为了检测体内和体外成牙本质细胞的分化，我们通过将GFP插入Dspp基因的靶向载体制备了Dspp Knock-In ES（Dspp-KI ES）细胞系。然后将Dspp-KI ES细胞注入囊胚中，获得大量嵌合体小鼠。培养6天的Dspp-KI ES衍生细胞也被移植到肾被膜中，4周后在移植组织中检测到YFP^+细胞。我们进一步尝试通过NC在体外将这些ESC分化为成牙本质细胞。

项目成果

Developmental origin of the earliest definitive hematopoietic progenitors

最早的定型造血祖细胞的发育起源

• 发表时间: 2009
• 作者: Yamane Toshiyuki;Yamazaki Hidetoshi
• 通讯作者: Yamazaki Hidetoshi

Cell fate regulation of the common primitive-definitive hematopoietic progenitors

常见原始定型造血祖细胞的细胞命运调控

• 发表时间: 2012
• 作者: 山根利之;鷲野亜矢;山崎英俊
• 通讯作者: 山崎英俊

Developmental origin of the earliest hematolymphoid progenitors

最早的造血淋巴祖细胞的发育起源

• 发表时间: 2010
• 作者: Yamane Toshiyuki;Aya Washino;Yamazaki Hidetoshi
• 通讯作者: Yamazaki Hidetoshi

Contribution of neural crest and mesodermal cells to the moue bone-marrow and thymic hematopoiesis

神经嵴和中胚层细胞对小鼠骨髓和胸腺造血的贡献

• 发表时间: 2012
• 作者: Nawa Y;Kawahara K;Tancharoen S;Meng X;Sameshima H;Ito T;Masuda Y;Imaizumi H;Hashiguchi T;Maruyama I;角熊直樹
• 通讯作者: 角熊直樹

Lymphoid lineage potential and developmental origin of the earliest hematopoietic progenitors in mice

小鼠最早造血祖细胞的淋巴谱系潜力和发育起源

• 发表时间: 2010
• 作者: Toshiyuki Yamane;Aya Washino;and Hidetoshi Yamazaki
• 通讯作者: and Hidetoshi Yamazaki