Analysis of potential for differentiation of murine neural-crest derived cells in developing teeth and regeneration of hard tissues using their neural-crest derived cells.

使用神经嵴衍生细胞分析小鼠神经嵴衍生细胞在牙齿发育和硬组织再生中的分化潜力。

基本信息

批准号：
17591907
负责人：
YAMAZAKI Hidetoshi
金额：
$ 2.24万
依托单位：
Mie University (2006)Tottori University (2005)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

1) It is known that mesenchymal cells are derived from both neural crest (NC) cells and mesoderm. Especially, neural crest contributed in cranio-face including dental mesenchyme. By tracing NC-derived cells as LacZ^+ cells using PO-Cre/Rosa26R mice, we found that large numbers of NC cells contributed dental mesenchyme and their NC-derived dental mesenchyme differentiated into odontoblasts, chondrocyte-like cells and osteoblast-like cells. (Yamazaki, 2007) Furthermore, we suggested that both NC-derived cells and non-NC-derived cells contributed in dental mesenchyme. 2) By tracing mesoderm-derived cells as LacZ^+ cells using Mespl-cre/Rosa26Rmice, we found that mesoderm-derived cells contributed in dental mesenchyme. 3) We found that NC-derived cells that participated in thymic organogenesis had a potential to differentiate into melanocytes and neuron and glia using PO-Cre/Rosa26R mice. We suggested that both NC and non-NC derved cells contributed in the mesenchyme of the thymususing these mice (Yamazaki,2005). 4) We showed that N-rasG12V expression in neural crest specifically promoted manifestations of neurofibromatosis using neural crest specific gene expression (Saito, 2007).5) We established culture system of NC-derived melanocytes from murine embryonic stem (ES) cells. Using endothelin3 or c-Kit disrupted ES cells, we showed that the role of c-Kit and endothelin3 in melanocyte development (Aoki, 2005).6) It is difficult to avoid undesirable lineages cells when we culture ES cells into some lineages. We showed that enforced expression of PU.1 rescues osteoclastogenesis from ES cells lacking tal-1 that can not differentiate into hematopoietic cells. Therefore, we might regulate the potential for differentiation of ES cells using these systems. (Tsuneto, 2005)

1）众所周知，间充质细胞来源于神经嵴（NC）细胞和中胚层。特别是，神经嵴对颅面（包括牙齿间质）有贡献。通过使用 PO-Cre/Rosa26R 小鼠追踪 NC 衍生细胞作为 LacZ^+ 细胞，我们发现大量 NC 细胞贡献了牙齿间质，并且它们的 NC 衍生牙齿间质分化为成牙本质细胞、软骨细胞样细胞和成骨细胞样细胞。 (Yamazaki, 2007) 此外，我们认为 NC 衍生细胞和非 NC 衍生细胞都对牙齿间质有贡献。 2)通过使用Mespl-cre/Rosa26Rmice追踪中胚层来源的细胞作为LacZ^+细胞，我们发现中胚层来源的细胞在牙齿间充质中做出贡献。 3）我们发现，使用 PO-Cre/Rosa26R 小鼠，参与胸腺器官发生的 NC 衍生细胞具有分化为黑素细胞、神经元和神经胶质细胞的潜力。我们认为，NC 和非 NC 衍生细胞都对这些小鼠的胸腺间质有贡献 (Yamazaki,2005)。 4）我们利用神经嵴特异性基因表达表明神经嵴中的N-rasG12V表达特异性促进神经纤维瘤病的表现（Saito，2007）。5）我们建立了来自小鼠胚胎干（ES）细胞的NC衍生黑素细胞的培养系统。使用内皮素3或c-Kit破坏ES细胞，我们证明了c-Kit和内皮素3在黑素细胞发育中的作用（Aoki，2005）。6）当我们将ES细胞培养成某些谱系时，很难避免不良谱系细胞。我们发现，PU.1 的强制表达可以挽救缺乏 tal-1 且无法分化为造血细胞的 ES 细胞的破骨细胞生成。因此，我们可以使用这些系统来调节 ES 细胞的分化潜力。（常人，2005）