Analysis of potential for differentiation of murine neural-crest derived cells in developing teeth and regeneration of hard tissues using their neural-crest derived cells.

使用神经嵴衍生细胞分析小鼠神经嵴衍生细胞在牙齿发育和硬组织再生中的分化潜力。

基本信息

批准号：
17591907
负责人：
YAMAZAKI Hidetoshi
金额：
$ 2.24万
依托单位：
Mie University (2006)Tottori University (2005)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

1) It is known that mesenchymal cells are derived from both neural crest (NC) cells and mesoderm. Especially, neural crest contributed in cranio-face including dental mesenchyme. By tracing NC-derived cells as LacZ^+ cells using PO-Cre/Rosa26R mice, we found that large numbers of NC cells contributed dental mesenchyme and their NC-derived dental mesenchyme differentiated into odontoblasts, chondrocyte-like cells and osteoblast-like cells. (Yamazaki, 2007) Furthermore, we suggested that both NC-derived cells and non-NC-derived cells contributed in dental mesenchyme. 2) By tracing mesoderm-derived cells as LacZ^+ cells using Mespl-cre/Rosa26Rmice, we found that mesoderm-derived cells contributed in dental mesenchyme. 3) We found that NC-derived cells that participated in thymic organogenesis had a potential to differentiate into melanocytes and neuron and glia using PO-Cre/Rosa26R mice. We suggested that both NC and non-NC derved cells contributed in the mesenchyme of the thymususing these mice (Yamazaki,2005). 4) We showed that N-rasG12V expression in neural crest specifically promoted manifestations of neurofibromatosis using neural crest specific gene expression (Saito, 2007).5) We established culture system of NC-derived melanocytes from murine embryonic stem (ES) cells. Using endothelin3 or c-Kit disrupted ES cells, we showed that the role of c-Kit and endothelin3 in melanocyte development (Aoki, 2005).6) It is difficult to avoid undesirable lineages cells when we culture ES cells into some lineages. We showed that enforced expression of PU.1 rescues osteoclastogenesis from ES cells lacking tal-1 that can not differentiate into hematopoietic cells. Therefore, we might regulate the potential for differentiation of ES cells using these systems. (Tsuneto, 2005)

1）众所周知，间充质细胞均来自神经rest（NC）细胞和中胚层。尤其是，神经rest在包括牙齿间充质在内的颅面上做出了贡献。通过使用PO-CRE/ROSA26R小鼠将NC衍生的细胞作为LACZ^+细胞追踪，我们发现大量NC细胞贡献了牙齿间充质及其NC衍生的牙中质质，分化为牙糖细胞，软骨细胞状细胞和粘膜细胞和Osteoblast样细胞。（Yamazaki，2007年）此外，我们建议NC衍生的细胞和非NC衍生的细胞在牙科间充质中贡献。 2）通过使用Mespl-Cre/Rosa26rmice将中胚层衍生的细胞作为LACZ^+细胞追踪，我们发现中胚层衍生的细胞在牙齿间充质中贡献了。 3）我们发现使用PO-CRE/ROSA26R小鼠，参与胸腺器官发生的NC衍生细胞具有分化为黑色素细胞，神经元和神经胶质的潜力。我们提出，NC和非NC捐赠细胞在胸腺司孔的间充质中有贡献（Yamazaki，2005）。 4）我们表明，在神经crest中的N-Rasg12v表达使用神经Crest特异性基因表达特异性促进了神经纤维瘤病的表现（Saito，2007）.5）我们从鼠类胚胎茎（ES）细胞中建立了NC衍生的黑素细胞的培养系统。使用内皮素3或C-KIT破坏ES细胞，我们表明C-KIT和ENDOPHELIN3在黑素细胞发育中的作用（Aoki，2005）.6）.6）。当我们将ES细胞培养到某些谱系中时，很难避免不良的谱系。我们表明，PU.1强迫表达从缺乏无法区分造血细胞的TAL-1的ES细胞中拯救了破骨细胞生成。因此，我们可以使用这些系统来调节ES细胞分化的潜力。（Tsuneto，2005年）