Induction of differentiate into odontogenic cells from murine embryonic stem cell

鼠胚胎干细胞诱导分化为牙源细胞

基本信息

批准号：
13671900
负责人：
YAMAZAKI Hidetoshi
金额：
$ 1.86万
依托单位：
Tottori University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671900/
关键词：
Neural crest Embryonic stem cell Regeneration of tooth In virto culture system Odontoblast Mouse Osteoclast Osteoblast 試験管内分化誘導歯の発生デンチンシアロプロテイン試験内分化誘導遺伝子導入マウス

项目摘要

We have found that odontoblasts are derived from neural crest cells using P0-cre mice and Rosa26R mice. In these mice, neural crest cells are easily detected as LacZ+ cells and these mice enable us to isolate LacZ+ neural crest cells in vivo. Furthermore, we have found that BMP signalings are important for molar cusp formation in tooth molar organ culture. We also developed the culture system of embryonic stem cells to induce the differentiation of osteoclasts and osteoblasts. The results are shown as follows.1) Using P0-cre mice and Rosa26R mice, neural crest cells and their progeny can be easily detected as LacZ+ cells and can be isolated. Multipotent neural crest cells with a potential of differentiate into melanocyte and neuroal cells were present in the fetal thymus.2) Using soluble formed BMP type I receptor to inhibit BMP signaling in tooth organ culture, the inhibition of BMP signaling at both E13.5 and E16.5 tooth buds leads to abnormal cusp formation. These results indicate that BMP signaling is essential for the proper cusp formation in all stags of tooth morphogenesis.3) We developed the culture system of embryonic stem cells to induce the differentiation of osteoclasts and osteoblasts.We have already published the papers 3) and the manuscripts 1) 2) have been submitted. Recently, we established the culture system of melanocytes, which are derived from neural crest cells are induced from murine embryonic stem cells. Furthermore, we also established the Dsp-LacZ mice, which expressed LacZ in odontoblast specially. Therefore, we may establish new system of differentiation into odontoblast from embryonic stem cell through neural crest cells.

我们发现，使用P0-CRE小鼠和Rosa26R小鼠源自神经rest细胞的ODONTOBLASTS。在这些小鼠中，很容易检测到神经rest细胞为LACZ+细胞，这些小鼠使我们能够在体内分离LACZ+神经rest细胞。此外，我们发现BMP信号对于牙齿摩尔器官培养中的摩尔尖缘很重要。我们还开发了胚胎干细胞的培养系统，以诱导破骨细胞和成骨细胞的分化。结果如下。1）使用P0-CRE小鼠和Rosa26R小鼠，神经rest细胞及其后代很容易被检测为LACZ+细胞，并且可以分离。在胎儿胸腺中存在具有分化为黑色素细胞和神经细胞潜力的多能神经rest细胞。2）使用可溶性的I型I型受体形成的可溶性BMP I型受体来抑制牙恩器官培养中的BMP信号，在E13.5和E13.5和E13.5和E13.5和E13.5和BMP信号的抑制E16.5牙齿芽会导致异常尖端形成。这些结果表明，BMP信号对于在牙齿形态发生的所有雄鹿中的适当尖端形成至关重要。3）我们开发了胚胎干细胞的培养系统以诱导整骨细胞和骨细胞的分化。手稿1）2）已提交。最近，我们建立了源自神经rest细胞的黑素细胞培养系统是由鼠类胚胎干细胞诱导的。此外，我们还建立了DSP-LACZ小鼠，该小鼠特别表达了Odontoblast的LACZ。因此，我们可以通过神经rest细胞建立从胚胎干细胞中分化为牙胶细胞的新系统。