Oncogenic factors in Chinese gastric lymphoma

中国人胃淋巴瘤的致癌因素

• 批准号: 11691216
• 负责人: HASUI Kazuhisa
• 金额: $ 2.43万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11691216/
• 关键词: Gastric lymphoma; Helicobacter pylori; China; IgH CDR3; direct DNA sequencing; EBV; Web-assisted DNA analysis; design of oligonucleotide probes; Gastric ulcer; Web-assisted design of ISH oligoprobe; Epstein-Barr virus(EBV); EBER-1 in-situ hybri

1, This research project held a symposium to report the prevalence of Helicobacter pyloris (HP) infection in China, no difference in clinicopathology between Chinese and Japanese gastric lymphomas, and the present state of researches of immunoglobulin heavy chain gene variable region (Ig H V) in gastric lymphoma cell.2, In Chinese gastric lymphoma cases exsmined, there were many advanced cases and some cases with giant lymphoma cells.3, CD68- and/or thymidine phosphorylase (TP)-positive dendritic cells among gastric lymphoma cells suggested the participation of intermingling T cell in histogenesis of the gastric B-cell lymphomas.4, IgH V DNA sequences analyzed suggested bacterial co-infection in development of gastric MALT type lymphoma, and homology to various human genes and on-going somatic mutation in development of high-grade gastric lymphomas.5, Expression of Inducible nitric oxide synthase (iNOS), which produces nitric oxide (NO) with mutagenicity, was examined in the Whole-body … More lymph apparatus, HP-related gastric ulcer and gastric lymphomas. (1) The expression of iN0S in germinal centers had a close relation with HP infestation. (2) Lewis X/Y, lypopolysaccharides of HP body, deposits in the germinal center of the regional lymph nodes. (3) iNOS expressed in the intermingling germinal centers in gastric MALT type lymphoma. Then, HP pathogenity to the mucosal immunity and HP carcinogenicity to gastric MALT type lymphoma may appear in the germinal centers, where iNOS was induced by Lewis X/Y and produced NO.6, In some cases a few lymphoma cells with EBER-1 signals suggested that infection of Epstein-Barr virus (EBV) infection is one of progression factors in the oncogenesis of gastric lymphoma, although most of the lymphoma cells lost the EBV genome.7, This research project developed the web-utilized method that designs oligonucleotides probes detecting pathological condition of API2 and MALT-1 genes, of which the chimeric gene was found in NP-non-related MALT type lymphoma. Less

1、本课题召开研讨会，报告中国幽门螺杆菌（HP）感染情况、中国与日本胃淋巴瘤临床病理无差异、免疫球蛋白重链基因可变区（Ig H V）研究现状2、中国胃淋巴瘤病例中，晚期病例较多，部分病例伴有巨淋巴瘤细胞。3、CD68-和/或胃淋巴瘤细胞中胸苷磷酸化酶 (TP) 阳性的树突状细胞提示混合 T 细胞参与胃 B 细胞淋巴瘤的组织发生。 4、IgH V DNA 序列分析提示胃 MALT 型淋巴瘤发生过程中存在细菌共感染，与各种人类基因的同源性以及高级胃淋巴瘤发展中正在进行的体细胞突变。5、诱导型一氧化氮合酶的表达iNOS产生具有致突变性的一氧化氮(NO)，在全身淋巴管、HP相关性胃溃疡和胃淋巴瘤中进行了检测(1)iNOS在生发中心的表达与生发中心有密切关系。 (2) HP体的Lewis X/Y脂多糖沉积于区域淋巴结的生发中心。 (3) iNOS在混合体中表达。然后，HP对粘膜免疫的致病性和HP对胃MALT型淋巴瘤的致癌性可能出现在生发中心，其中iNOS被Lewis X/Y诱导并产生NO.6，在某些情况下a少数淋巴瘤细胞带有 EBER-1 信号，表明 Epstein-Barr 病毒 (EBV) 感染是胃淋巴瘤发生的进展因素之一，尽管大多数淋巴瘤细胞失去了 EBER-1 信号。 EBV基因组。7，该研究项目开发了一种利用网络设计寡核苷酸探针来检测API2和MALT-1基因病理状况的方法，其中在NP非相关MALT型淋巴瘤中发现了嵌合基因。

项目成果

蓮井和久: "HTLV-1キャリアーの皮膚anaplastic large cell lymphomaの一例"皮膚のリンフォーマ. 19. 151-154 (2000)

Kazuhisa Hasui：“HTLV-1 携带者皮肤间变性大细胞淋巴瘤的一个例子”皮肤淋巴瘤。19. 151-154 (2000)

Hasui K, Sato E, Kitazono M, Akiyama S, Tanaka Y, Higashi M, Taki C, Sakoda A, Tashiro Y, Shirahama H: "Immunohistochemical analysis of thymidine phosphorylase overexpression in malignant lymphomas (ML), especially in adult T-cell leukemia lymphoma (ATLL)

Hasui K、Sato E、Kitazono M、Akiyama S、Tanaka Y、Higashi M、Taki C、Sakoda A、Tashiro Y、Shirahama H：“恶性淋巴瘤 (ML) 中胸苷磷酸化酶过度表达的免疫组织化学分析，特别是在成人 T 细胞中

Hasui K, Higashi M, Tachi C, Setoyama M, Kanzaki T, Hata Y, Kouno H, Maeda K, Tashiro Y, Shirahama H, Sato E: "A case of cutaneous anaplastic large cell lymphoma in a HTLV-1 carrier. (in Japanese)."Lymphoma of the Skin XIX. 19. 151-154 (2000)

Hasui K、Higashi M、Tachi C、Setoyama M、Kanzaki T、Hata Y、Kouno H、Maeda K、Tashiro Y、Shirahama H、Sato E：“HTLV-1 携带者皮肤间变性大细胞淋巴瘤一例。（

Hasui K, Nakamura K, Sato E, Nakagawa M, Izumo S: "Expression of inducible nitric oxide synthase in epithelial cells, mucosa-associated lymphoid tissue, and regional lymph nodes of the stomach with Helicobacter pylori-associated peptic ulcer."DENDRITIC CE

Hasui K、Nakamura K、Sato E、Nakakawa M、Izumo S：“诱导型一氧化氮合酶在上皮细胞、粘膜相关淋巴组织和幽门螺杆菌相关消化性溃疡胃区域淋巴结中的表达。”DENDRITIC CE

Hasui K, Higashi M, Long H, Yashiki S, Nakamura T, Sueyoshi K, Tashiro Y, Shirahama H, Sato E: "Constant extraction of DNA from paraffin-embedded gastric surgical and biopsy specimens for polymerase chain reaction (PCR) analysis."Med J.Kagoshima U. 51(Sup

Hasui K、Higashi M、Long H、Yashiki S、Nakamura T、Sueyoshi K、Tashiro Y、Shirahama H、Sato E：“从石蜡包埋的胃手术和活检标本中持续提取 DNA，用于聚合酶链反应 (PCR) 分析。