In-situ-hybridization analysis of HTLV-1-related diseases, employing highly biotinylated probes synthesized by polymerase chain reaction

采用聚合酶链式反应合成的高度生物素化探针对 HTLV-1 相关疾病进行原位杂交分析

基本信息

批准号：
05670176
负责人：
HASUI Kazuhisa
金额：
$ 1.41万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

This project aimed to detect HTLV-1 proviral DNA and its signals in cellular composition of HTLV-1-related diseases, especially malignant lymphomas (MLs), by means of in-situ-hybridization (ISH) employing highly biotinylated probes synthesized by polymerase chain reaction. The ISH failed to detect HTLV-1 proviral DNA but succeeded to detect its signals in paraffin sections of HTLV-1-related cell line cells and human ML cells. The signals of HTLV-1 proviral DNA pX Tax region were recognized the most frequently in T-cell pleomorphic lymphoma (T-Pleo) including adult T-cell leukemia/lymphoma (ATLL). The signals in some of low-grade T-cell MLs and the other MLs suggested the existence of HTLV-1-related MLs other than ATLL and the promotion from HTLV-1-related low-grade T-cell MLs to ATLL.Antigen-retrieval immunohistochemical detection of HTLV-1 p40tax was performed in the MLs revealing the signals in ISH.Bur p40tax distributed in cytoplasm of ML cells in many T-cell MLs and in nuclei in a small number of T-cell MLs, suggesting that the direct comparision of the signals of HTLV-1 proviral DNA and the activation of host cell genes would fail to see the transactivating function of HTLV-1 p40tax. A small amount of p40tax in the nuclei of ATLL cells could be visualized by antigen-retrieval and ImmunoMax method. Then, the relation of the activation of HTLV-1 proviral DNA and host cell gens in HTLV-1-related diseases will be able tp be analyzed by means of the combination of the ISH and the antigen-retrieval and ImmunoMax method, considering the HTLV-1 p40tax's functions other than the transactivation.

该项目旨在通过使用高度生物素化探针通过聚合酶链链反应合成的，通过原位 - 杂交（ISH）检测HTLV-1前DNA及其在HTLV-1相关疾病（尤其是恶性淋巴瘤（MLS））中的细胞组成中，尤其是恶性淋巴瘤（MLS）。 ISH无法检测到HTLV-1前病毒DNA，但成功地检测了其在HTLV-1相关细胞细胞和人类ML细胞的石蜡切片中的信号。 HTLV-1病毒DNA PX税收区的信号在T细胞多形淋巴瘤（T-PLEO）中最常被认为是包括成年T细胞白血病/淋巴瘤（ATLL）的信号。 The signals in some of low-grade T-cell MLs and the other MLs suggested the existence of HTLV-1-related MLs other than ATLL and the promotion from HTLV-1-related low-grade T-cell MLs to ATLL.Antigen-retrieval immunohistochemical detection of HTLV-1 p40tax was performed in the MLs revealing the signals in ISH.Bur p40tax在许多T细胞ML中分布在ML细胞的细胞质中和少数T细胞ML中的核中，这表明HTLV-1前病毒DNA信号的直接比较以及宿主细胞基因的激活将无法看到HTLV-1 P40TAX的htlv-1 tlastivivivivive函数。 ATLL细胞核中少量的P40TAX可以通过抗原 - 恢复和免疫X方法可视化。然后，通过ISH的组合和HTLV-1 P40TAX功能以外的其他功能。