Academic research project on oncogenic factors in malignant lymphomas in the east-northern China

中国东北地区恶性淋巴瘤致癌因素学术研究项目

基本信息

批准号：
13576003
负责人：
HASUI Kazuhisa
金额：
$ 5.82万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13576003/
关键词：
Nasal NK T-cell lymphoma Apoptosis Background stromal cells Cell origin Premonitory lesions Cutaneous nasal type NK Pathology-specimen International co-operative study 中国悪性リンパ腫鼻NK細胞性リンパ腫 CD203 CD34 CD117 幹細胞の免疫組織化学分子病理学

项目摘要

This research-project analyzed pathology-specimens of nasal malignant lymphomas in the east-northern China by means of immunohistochemistry and EBER-1 in-situ hybridization and found more Epstein-Barr virus (EBV)-related NK-cell lymphomas in the order of tonsil, oral cavity and nose. In these nasal NK-cell lymphomas, expression of CD204, a receptor for apoptosis products, was noted in many macrophages in the background of the nasal NK-cell lymphomas, suggesting that degenerative tendency was that of apoptosis, although abnormal expression of p53 protein and high expression of phosphorylated p53 protein were not detected. For the next project, we checked immunohistochemistry of cleaved caspase-3 and found that the immunohistochemistry can detect cells falling in apoptosis before nuclear degeneration. In analysis of stromal cells in the background Chinese nasal NK-cell lymphomas, immature thymidine phosphorylase-positive stromal cells were noted in these lymphomas, when T-cell-dependent dendritic cells are seen in peripheral T-cell lymphomas. For the next project, we checked whether immunohistochemistry of stem cell 1 s markers can be applied to detect stem cells in the bone marrow, peripheral blood and the tissues and found that it is possible by this method to label tissue stem cells in human pathology-specimens. It was unknown in this project whether there were premonitory lesions of nasal NK-cell lymphomas, when it was shown in this project that nasal type NK-cell lymphomas were rare in China. In the period of this project, research ethical aspects of this project were prepared, since this project is an international one analyzing pathology specimens in the Chinese medicine.

该研究项目通过免疫组化和EBER-1原位杂交技术对中国东北地区鼻部恶性淋巴瘤的病理标本进行了分析，发现了更多与Epstein-Barr病毒（EBV）相关的NK细胞淋巴瘤，顺序为：扁桃体、口腔和鼻子。在这些鼻NK细胞淋巴瘤中，在鼻NK细胞淋巴瘤背景中的许多巨噬细胞中注意到CD204（一种凋亡产物的受体）的表达，表明退行性趋势是凋亡，尽管p53蛋白和未检测到磷酸化p53蛋白的高表达。在下一个项目中，我们检查了 cleaved caspase-3 的免疫组化，发现免疫组化可以检测到核变性前陷入凋亡的细胞。在对中国鼻 NK 细胞淋巴瘤背景基质细胞的分析中，在这些淋巴瘤中发现了未成熟的胸苷磷酸化酶阳性基质细胞，而在外周 T 细胞淋巴瘤中发现了 T 细胞依赖性树突状细胞。在下一个项目中，我们检查了干细胞1s标记物的免疫组化是否可以应用于检测骨髓、外周血和组织中的干细胞，发现通过这种方法可以标记人体病理中的组织干细胞——标本。本项目尚不清楚鼻型NK细胞淋巴瘤是否存在先兆病变，但本项目显示鼻型NK细胞淋巴瘤在中国很少见。在该项目期间，由于该项目是分析中医病理标本的国际项目，因此对该项目的研究伦理方面进行了准备。

项目成果

期刊论文数量（37）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Direct DNA Sequence Analysis of Immunoglobulin Heavy Chain Gene Variab1e Region in Chinese Gastric Lymphomas.

中国胃淋巴瘤免疫球蛋白重链基因可变区的直接 DNA 序列分析。

DOI：
发表时间：
2002
期刊：
Lymphoreticular Cells and Diseases. Proceedings of the Seventh Japanese-Korcan Lymphoreticular Workshop
影响因子：
0
作者：
Hasui K
通讯作者：
Hasui K

Direct DNA Sequence Analysis of Immunoglobulin Heavy Chain Gene Variable Region in Chinese Gastric Lymphomas.

中国胃淋巴瘤免疫球蛋白重链基因可变区的直接DNA序列分析。

DOI：
发表时间：
2002
期刊：
Lymphoreticular Cells and Diseases. Proceedings of the Seventh Japanese-Korean Lymphoreticular Workshop
影响因子：
0
作者：
Hasui K;Li F;Jia XS;Nakamura T;Iwamoto H;Sato E;Nakagawa M;Izumo S;Murata F
通讯作者：
Murata F

Immunohistochemical analysis of the pathogenicity of helicobacter pylorinfection : Excess nitric oxide induced indirectly by Lewis X and Y is the cause of the pathogenicity of helicobacter pylori Infestation in the stomach.

幽门螺杆菌感染致病性的免疫组织化学分析：Lewis X和Y间接诱导的过量一氧化氮是胃内幽门螺杆菌感染致病性的原因。