Development of preditection method to prevent occurrence of adult T-cell leukemia/lymphoma (ATLL)

开发预防成人T细胞白血病/淋巴瘤（ATLL）发生的预测方法

• 批准号: 13557017
• 负责人: HASUI Kazuhisa
• 金额: $ 8.77万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13557017/
• 关键词: Peripheral blood tissue specimen (PBTS); Leukemia; Adult T-cell leukemia; lymphoma (STLL); HTLV-1 carrier; Supersensitive immunohistochemistry; p53 protein; Phosphorylated p53 protein; Surface temperature of slide glass; Web DNA Database; HTLV-1; Peripheral blood tissue specimen (PBTS); Leukemia; Adult T-cell leukemia; lymphoma (STLL); HTLV-1 carrier; Supersensitive immunohistochemistry; p53 protein; Phosphorylated p53 protein; Surface temperature of slide glass; Web DNA Database; HTLV-1

This project performed following investigations, aiming to predict occurrence of adult T-cell leukemia/lymphoma (ATLL). 1,Peripheral blood tissue specimens (PBTS) of leukemia including ATLL, HTLV-1 carriers and the other lymphocytosis were prepared. 2,Supersensitive immunohistochemistry, new simplified catalyzed signal amplification (nsCSA) system, was established. 3,By the nsCSA system employing anti-HTLV-1 Tax antibody, it was shown that an extremely small amount of Tax was expressed in ATLL cells in tissue specimens includings PBTS. 4,As neoplastic features of HTLV-1-infected cells, expression of Ki67 antigen and p53 protein was examined by the nsCSA system. (1)In HTLV-1 carriers, segmented nuclei of granulocytes were labeled by anti-Ki67 antigen antibody, suggesting an effect of HTLV-1 infection on hematopoiesis. (2)The nsCSA system of p53 1801 antibody detected expression of p53 related to apoptosis, since Ser46 in p53 regulating its function in apoptosis is labeled by p53 1801 an … More tibody. (3)Phosphorylated Ser392 in p53 was labeled by the nsCSA system of p53Phos in ATLL, HTLV-1 carriers and infectious mononucleosis, suggesting inactivation of p53. (4)Expression of p53Phos and p53 1801 antibody was significantly observed in advam\ncing age of HTLV-1 carriers (Kruskal-Walli test). 5,Multi-immunostaing method by an autoimmunostainer was established by introducing classic glycine treatment and blocking non-specific reaction of primary antibody. 6, By the DNA database on the web, it was possible to determine DNA sequence of oligonucleotide probe detecting mRNA of the target DNA. It was known that hybridization solution and detection system to visualize hybridized oligonucleotide probes were important factors in mRNA in-situ hybridization. By means of a sensor of slide glass surface temperature, it was possible to control temperature of tissue section on the slide glass in in-situ PCR method. The next stage detection system expects introduction of the nsCSA system (291 words) Less

该项目进行了调查，旨在预测成人T细胞白血病/淋巴瘤（ATLL）的发生。 1，制备了包括ATLL，HTLV-1载体和其他淋巴细胞增多的白血病的外周血组织标本（PBT）。 2，建立了超敏感的免疫组织化学，新的简化催化信号扩增（NSCSA）系统。 3，通过使用抗HTLV-1税收抗体的NSCSA系统，证明在包括PBT在内的组织样品中的ATLL细胞中表达了极少数的税收。 4，作为HTLV-1感染细胞的肿瘤特征，NSCSA系统检查了Ki67抗原和p53蛋白的表达。 （1）在HTLV-1载体中，通过抗KI67抗原抗体标记粒细胞的分段核会，这表明HTLV-1感染对造血的影响。 （2）p53 1801的NSCSA系统检测到与凋亡相关的p53的表达，因为p53在p53中的Ser46在p53中的Ser46被p53 1801标记为p53 1801 an…更多的tibody。 （3）p53中磷酸化的Ser392在ATLL，HTLV-1载体和感染性单核细胞增多症中的NSCSA系统标记，表明p53失活。 （4）在HTLV-1载体的Advam \ Ring年龄（Kruskal-Walli检测）中，显着观察到p53phos和p53 1801抗体的表达。 5，通过引入经典甘氨酸处理并阻止原代抗体的非特异性反应来建立通过自身免疫剂的多免疫剂。 6，通过网络上的DNA数据库，可以确定靶DNA的mRNA的寡核苷酸探针的DNA序列。众所周知，可视化杂交寡核苷酸问题的杂交解决方案和检测系统是mRNA现场杂交的重要因素。通过幻灯片玻璃表面温度的传感器，可以在原位PCR方法中控制幻灯片玻璃上组织截面的温度。下一个阶段检测系统期望引入NSCSA系统（291个单词）少