Two-dimensional high performance liquid chromatography in microfluidic chip devices

微流控芯片装置中的二维高效液相色谱

• 批准号: 371350234
• 负责人: Professor Dr. Detlev Belder
• 金额: --
• 依托单位: Institut für Analytische Chemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/371350234?language=en
• 关键词: Two; dimensional; performance; liquid; chromatography

The main scientific goal within the project -Two-dimensional high performance liquid chromatography in microfluidic chip devices- is the development of a miniaturized analysis system for fast analytical characterization of complex sample mixtures by multidimensional chip-based chromatography. Central aspect of the research work is the dead volume-free coupling of two pressure-driven chromatography units with particulate stationary phase by applying lab-on-a-chip technology. With this approach based on highly efficient coupling of chromatographic columns the great potential of multidimensional HPLC in microfluidic chips will be systematically investigated. Thereby, it should be possible to exploit the benefits of multidimensional nano-HPLC and to smartly bypass the technical hurdles of this technology, which are well documented in literature. Within the project microfluidic systems for two-dimensional chip chromatography are developed and the possibilities and limitations of this promising technology will be evaluated in detail.

项目中的主要科学目标 - 微流体芯片设备中的TWO高效液相色谱 - 是通过基于多维芯片色谱法对复杂样品混合物快速分析的小型化分析系统的开发。研究工作的主要方面是通过应用实验室芯片技术的两个压力驱动色谱单元与颗粒固定相的无量耦合。使用这种方法，基于色谱柱的高效耦合，将系统研究多维HPLC在微流体芯片中的巨大潜力。因此，应该有可能利用多维纳米-HPLC的好处，并巧妙地绕过这项技术的技术障碍，这些技术在文献中已经有充分的文献记载。在项目中，开发了用于二维芯片色谱的微流体系统，并将详细评估这种有前途的技术的可能性和局限性。

项目成果

Multiple Heart-Cutting Two-Dimensional Chip-HPLC Combined with Deep-UV Fluorescence and Mass Spectrometric Detection.

多中心切割二维芯片高效液相色谱结合深紫外荧光和质谱检测

• DOI: 10.1021/acs.analchem.9b05206
• 发表时间: 2020
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: S. K. Piendl;D. Geissler;L. Weigelt;D. Belder
• 通讯作者: D. Belder

Integration of segmented microflow chemistry and online HPLC/MS analysis on a microfluidic chip system enabling enantioselective analyses at the nanoliter scale.

• DOI: 10.1039/d1lc00078k
• 发表时间: 2021-05
• 期刊: Lab on a chip
• 影响因子: 6.1
• 作者: S. Piendl;T. Schönfelder;Matthias Polack;Laura Weigelt;T. van der Zwaag;T. Teutenberg;E. Beckert;D. Belder

Unveiling Organocatalysts Action – Investigating Immobilized Catalysts at Steady‐State Operation via Lab‐on‐a‐Chip Technology

• DOI: 10.1002/cctc.202101148
• 发表时间: 2021-09
• 期刊: ChemCatChem
• 影响因子: 4.5
• 作者: Hannes Westphal;Rico Warias;H. Becker;Matthias Spanka;D. Ragno;R. Gläser;C. Schneider;A. Massi;D. Belder