Digital microfluidics (DMF) for SERS analysis on a chip with EWOD-/(w)OEW-actuated analyte droplets

使用 EWOD/(w)OEW 驱动的分析物液滴在芯片上进行 SERS 分析的数字微流体 (DMF)

基本信息

批准号：
366065598
负责人：
Professor Dr. Detlev Belder
金额：
--
依托单位：
Institut für Analytische Chemie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2017
资助国家：
德国
起止时间：
2016-12-31 至 2021-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/366065598?language=en
关键词：
Digital microfluidics DMF SERS analysis

项目摘要

Electrically conducting fluid droplets can be actuated via electrowetting on dielectric (EWOD) and opto-electrowetting (OEW). In principle, the OEW control lightwave might even be waveguided within the layer sequence beneath the droplet (idea Fouckhardt, waveguide-based OEW = wOEW). An important application is represented by droplet manipulation in digital microfluidics (DMF, lab on a chip 2.0) in chemical chip-sized laboratories.An obstacle for a break-through of digital microfluidics in miniaturized chemistry is given by a lack of sensitive, non-destructive methods for detection, allowing for the doubtless identification of chemical substances in discrete droplets on a DMF chip. In the project proposed here surface-enhanced Raman scattering or spectroscopy (SERS) shall be employed for this purpose on a chip for the first time (idea Belder), which shall make well evaluable vibrational spectra possible. By the interaction of the analyte molecules (within the droplets) e. g. with metallic nanoparticles dispersed in the droplets high signal amplifications should be obtainable.The undertaking is aimed at the first-time combination of DMF and SERS and the concurrent droplet actuation by EWOD-OEW-wOEW. By the optical initiation of the droplet movement new opportunities for the realization of unpretentious lab on a chip systems should arise.The cooperation of the groups Belder and Fouckhardt should provide for synergies, which make a break-through of the DMF technology in the context of chemical lab-chips possible. The Belder group focuses on SERS detection within DMF systems. The Fouckhardt group concentrates on further development of droplet actuation via EWOD and OEW, preferably up to wOEW.Moreover, in close cooperation the potential of solid metallic SERS targets is to be investigated, which might simultaneously be used as electrical contacts or mirror layers for the EWOD-(w)OEW technologies. Multifunctionality of components is to be considered as inherently positive for further miniaturization of DMF lab chips.In principle, for any of the SERS target alternatives any of the mentioned actuation variants, i. e. EWOD, OEW, or wOEW, might be used. The (preliminary) allocation in the work program is owed to the temporal coordination of work packages of both groups.In case of need, for droplet actuation OEW (compared with wOEW) or even EWOD (compared with wOEW and OEW) might act as a fall-back position. Even the combination of DMF and SERS and EWOD actuation would be new.

电导传导流体液滴可以通过电介质（EWOD）和光电旋转（OEW）上的电解进行驱动。原则上，OEW控制灯波甚至可能在液滴下方的层序列中波导（Idea fouckhardt，基于波导的OEW = WOEW）。一个重要的应用是在化学芯片大小实验室中的数字微流体（DMF，芯片2.0实验室）中的液滴操纵来表示的。缺乏敏感的，非敏感的，非敏感的，非 - 破坏性的检测方法，允许在DMF芯片上无疑鉴定出离散液滴中的化学物质。在此处提出的项目中，应首次将表面增强的拉曼散射或光谱法（SER）用于此目的（Idea Belder），这将使可评估的振动光谱成为可能。通过分析物分子的相互作用（在液滴中）e。 g。应获得金属纳米颗粒分散在液滴中的高信号放大。该承诺的目的是针对DMF和SERS的首次组合以及Ewod-oew-woew的同时进行的液滴致动。通过液滴运动的光学启动，应出现在芯片系统上实现朴实实验室的新机会。贝尔德和福克哈特团体的合作应提供协同作用，这使得在DMF技术的背景下进行了脱离DMF技术化学实验室芯片可能。贝尔德组专注于DMF系统中的SERS检测。 Fouckhardt组集中于通过EWOD和OEW进一步开发液滴致动，最好是woew。此外，将要研究固体金属SERS目标的潜力，可以同时使用固体金属SERS目标，可以同时用作电气触点或镜像层EWOD-（W）OEW技术。组件的多功能性应被认为是对DMF实验室芯片的进一步微型化的固有积极性。 e。可以使用EWOD，OEW或WOEW。工作计划中的（初步）分配归功于两组的工作包的时间协调。在需要的情况下，用于液滴驱动OEW（与WOEW相比）甚至EWOD（与WOEW和OEW相比）可能充当后卫位置。即使是DMF和SERS和EWOD驱动的组合也是新的。