Time resolved fluorescence detection utilising two photon excitation in microfluidic separation systems

在微流体分离系统中利用两个光子激发进行时间分辨荧光检测

• 批准号: 222164465
• 负责人: Professor Dr. Detlev Belder
• 金额: --
• 依托单位: Institut für Analytische Chemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/222164465?language=en
• 关键词: Time; resolved; fluorescence; detection; utilising

This project focuses on the development of a novel approach for label-free detection of tiny sample amounts in chip-based separation systems. This will be achieved by two photon fluorescence excitation combined with time resolved fluorescence detection of the emission light. In comparison to common single photon excitation in the deep UV-spectral region, this should enable label free detection in economic chip materials which are not transparent below 300nm, such as borosilicate glass or plastics. On the fly determination of fluorescence life times will be utilized for reliable peak assignment in chip electrophoresis. The potential of two photon excitation in microfluidic systems will be evaluated systematically with of a high performance system for fluorescence excitation and detection. Finally the developed systems and methods will be evaluated in several application areas, such as Bionanalytics, drug analysis and integrated organic synthesis.

该项目的重点是开发一种新型方法，用于在基于芯片的分离系统中无标签的样品量。这将通过两种光子荧光激发与发射光的时间分辨荧光检测相结合来实现。与深紫外线区域中的常见单光子激发相比，这应该在不透明的300nm（例如硼硅酸盐玻璃或塑料）中实现经济芯片材料中的无标签检测。 在果定测定时，荧光寿命的时间将用于芯片电泳中的可靠峰分配。微流体系统中两个光子激发的电势将通过高性能系统进行系统评估，以进行荧光激发和检测。最后，将在几个应用领域进行评估开发的系统和方法，例如Bionanalytics，药物分析和综合有机合成。