MRI: Acquisition of an Open Access Shared-Use MALDI-TOF/TOF Mass Spectrometer

MRI：购买开放共享的 MALDI-TOF/TOF 质谱仪

• 批准号: 2117934
• 负责人: Travis Bailey
• 金额: $ 40万
• 依托单位: Colorado State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2117934&HistoricalAwards=false
• 关键词: MRI; Acquisition; Open; Access; Shared; MRI; Acquisition; Open; Access; Shared

This award is supported by the Major Research Instrumentation and the Chemistry Research Instrumentation programs. Colorado State University is acquiring a matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF-TOF) mass spectrometer to support Professor Travis Bailey and colleagues Claudia Boot, Karolien Denef and Garrett Miyake. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex samples. MALDI TOF combines gentle ionization (ideal for producing intact ions of peptides, proteins, nucleic acids, carbohydrates, synthetic polymers, and other similarly sized species) with a detection mode that offers an excellent balance between sensitivity and accuracy across a wide mass range. This highly sensitive technique allows identification and determination of the structure of molecules in a complex mixture. In the time-of-flight (TOF) method of mass spectrometry, the mass-to-charge ratio of an ion is determined by the way in which ions are accelerated by an electric field of known strength. The velocity of the ion depends on the mass-to-charge ratio (heavier ions of the same charge reach lower speeds). The time that the ions take to reach a detector at a known distance is measured. This time depends on the velocity of the ion, and therefore is a measure of its mass-to-charge ratio. From this ratio and known experimental parameters, the ion can be characterized. The tandem capabilities (TOF-TOF) increase the characterization capabilities. The acquisition strengthens the research infrastructure at the university and regional area. The instrument broadens participation by involving diverse students with this modern analytical technique. It is also used by collaborators at the various campuses and other regional institutions. The MALDI-TOF-TOF is also employed by undergraduate and graduate students. It is also used in a postdoc instrumentalist training program and annual schools on instrumental methods.The award of the MALDI-TOF-TOF mass spectrometer is aimed at enhancing research and education at all levels. It is especially useful for studies in the development of biological imaging tools and identifying synthesized biological tissues. The instrumentation is also used for controling pharmaceutical delivery agents and nanocarriers and studying heat-reflective coatings. The instrument serves researchers preparing metal-free catalysts and high-value self-sustainable polymers and those using diagnostics and drugs to potentially fight infectious disease. The mass spectrometer is also utilized in the preparation of efficient carbon capture membranes and for the study of blood compatible surfaces and polymeric wound healing scaffolds. Additionally, it is employed in characterizing prepared advanced hydrogel networks and for studying energy storage materials.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项得到了主要的研究工具和化学研究工具计划的支持。科罗拉多州立大学（Colorado State University）正在购买矩阵辅助的激光解吸/电离串联飞行时间（MALDI-TOF-TOF）质谱仪，以支持Travis Bailey教授和同事Claudia Boot，Karolien Denef和Garrett Miyake。通常，质谱法（MS）是用于识别和表征复杂样品中少量化学物种的关键分析方法之一。 Maldi TOF结合了温和的电离（非常适合生产肽，蛋白质，核酸，碳水化合物，合成聚合物和其他类似尺寸的物种的完整离子和检测模式，该模式在广泛的质量范围内具有良好的平衡。这种高度敏感的技术允许鉴定和确定复杂混合物中分子的结构。在飞行时间（TOF）的质谱法（TOF）方法中，离子的质量与电荷比由离子通过已知强度的电场加速的方式确定。离子的速度取决于质量与电荷比（相同电荷的较重离子达到较低的速度）。测量离子在已知距离处到达检测器所需的时间。这次取决于离子的速度，因此是其质量与电荷比的度量。从该比率和已知的实验参数，可以表征离子。串联功能（TOF-TOF）提高了表征能力。此次收购加强了大学和地区地区的研究基础设施。该乐器通过让多样化的学生使用这种现代分析技术来扩大参与。各个校园和其他区域机构的合作者也使用了它。 MALDI-TOF-TOF也受到本科生和研究生的雇用。它也用于在工具方法的博士后乐器培训计划和年度学校中使用。MALDI-TOF-TOF-TOF质谱仪的奖励旨在增强各个级别的研究和教育。它对于研究生物成像工具的开发和识别合成的生物组织特别有用。该仪器还用于控制药物输送剂和纳米载体和研究热涂层。该仪器为准备无金属催化剂和高价值自我耐受性聚合物以及使用诊断和药物与感染性疾病作斗争的研究人员提供服务。质谱仪还用于制备有效的碳捕获膜以及研究血液兼容表面和聚合物伤口愈合支架的研究。此外，它还用于表征准备好的高级水凝胶网络并研究储能材料。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛的影响审查标准通过评估来支持的。