I-Corps: High Content Label Free Imaging of Tissue using a Novel Laser Source

I-Corps：使用新型激光源对组织进行高内涵无标签成像

• 批准号: 1931891
• 负责人: Stephen Boppart
• 金额: $ 5万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-06-15 至 2020-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1931891&HistoricalAwards=false
• 关键词: Corps; Content; Label; Free; Imaging

The broader impact/commercial potential of this I-Corps project encompasses laser science, microscope technology, and the entire spectrum of biological imaging of live tissues, cells, and microorganisms. This includes basic life science and environmental research where advanced microscopes and cell measurement instruments are used routinely to collect data from cell cultures, animals, and human specimens. Most of these measurements currently require the use of expensive chemical or biological "labels" to identify the targets of interest. This labeling process is time consuming, expensive, and often results in irreversible damage to the specimen. This technology largely eliminates the need for these labels, substantially reducing both the cost and time required to perform experiments. This means that precious public and private research funds will accomplish more. In addition, the potential commercialization plan for this technology includes offering it to existing microscope and instrument manufacturers so that it can readily be integrated into existing systems.This I-Corps project and its intellectual merit involves an advanced technology for rapidly capturing images of live tissue at microscopic resolution, and immediately extracting a wealth of information about the viability and function of the cells and tissue from those images, all without the need for chemical staining or tissue preparation. This technology is the result of over a decade of research conducted at a major university where the research team created a new programmable laser light source that tailors and launches short pulses of light to interact with specific molecular components within the live tissue, essentially making blood vessels, different cells, and cancerous tissue "light-up" with unique light signatures. By combining this laser source with an advanced microscope and software also developed by the research team, a next-generation imaging device has been developed that could dramatically improve the accuracy and speed, and lower the cost of histology, which is currently used to microscopically assess dead, fixed, and stained tissue sections.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该I-Corps项目的更广泛的影响/商业潜力涵盖了激光科学，显微镜技术以及活组织，细胞和微生物的生物成像的整个范围。这包括基本的生命科学和环境研究，在该研究中，通常使用高级显微镜和细胞测量工具来从细胞培养物，动物和人类标本中收集数据。目前，这些测量大多数都需要使用昂贵的化学或生物“标签”来识别感兴趣的目标。这个标签过程是耗时，昂贵的，而且通常会对样品造成不可逆转的损害。这项技术在很大程度上消除了对这些标签的需求，从而大大减少了执行实验所需的成本和时间。这意味着宝贵的公共和私人研究基金将取得更多成就。 此外，该技术的潜在商业化计划包括将其提供给现有的显微镜和仪器制造商，以便它可以轻松地集成到现有系统中。本I-Corps项目及其智力优点涉及一项先进的技术，可快速捕获微观分辨率的活组织图像，并立即从这些需要的细胞和组织中提取物质和组织，同样是在这些方法中，这些信息立即用于化学或组织的功能，同样或组织。 这项技术是在一所大型大学进行的十多年研究的结果，研究团队创建了一个新的可编程激光源，该激光光源量身定制和发射短脉冲，以与活组织中的特定分子成分相互作用，从本质上制造血管，不同的细胞，不同的细胞和独特的光签名“ Light-Up”。通过将该激光源与研究团队还开发的高级显微镜和软件相结合，已经开发了一种下一代成像设备，可以极大地提高其准确性和速度，并降低组织学成本，该组织学成本用于微观评估死亡，固定，固定和染色的组织，这些奖项反映了NSF的构建范围和构建范围的构建，该奖项的构建范围是良好的构建，其范围是依据的构建，其构建的范围是依据的，该奖项的范围是依据的，该奖项的范围均具有良好的构建功能。 标准。