Advanced Optical Imaging of 3-D Cell Dynamics in Engineered Skin

工程皮肤 3D 细胞动力学的先进光学成像

• 批准号: 1033906
• 负责人: Stephen Boppart
• 金额: $ 60万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1033906&HistoricalAwards=false
• 关键词: Advanced; Optical; Imaging; Cell; Dynamics

1033906BoppartDespite important advances within the past decade, current tissue engineered skin equivalents represent little more than a fragile, short-term dressing for patients that need viable skin replacements. The major weakness in current skin equivalents is that the constituent cells are cultured and applied under conditions that are very different from that of natural skin. It is believed that this is principally due to our limited understanding of the roles that 3-D scaffold topography and mechanical stimuli have on the intercellular organization, connectivity, and communication of engineered tissues. In this project an advanced integrated microscope capable of simultaneous optical coherence and multi-photon microscopy, and optical coherence elastography is utilized to uniquely visualize the structural and functional relationships of cells within 3-D engineered skin constructs, and measure the evolving biomechanical properties. Second, this project investigates and longitudinally images in 3-D the growth of engineered skin constructs on varying microtopographic substrates. This will provide fundamental insight into the mechanical influences at the dermal-epidermal junction on the keratinocytes and fibroblasts. Third, the effects of mechanical stimuli on these constructs will be investigated, defining how varying stimuli affect the 3-D cell dynamics and tissue organization over time. such stimulation effects will provide a more physiologically-relevant culture condition. Finally, this project will longitudinally image the cell and tissue responses in vivo following the grafting of the skin constructs to host pre-clinical models, contributing significantly to our understanding of how engineered tissue grafts interface with biological hosts.

1033906boppartdite在过去的十年内取得了重要的进步，目前的组织工程皮肤当量仅代表着需要可行的皮肤可行的患者的脆弱的短期敷料。当前皮肤当量的主要弱点是成分细胞在与自然皮肤截然不同的条件下进行培养和应用。人们认为，这主要是由于我们对3-D脚手架形态和机械刺激在工程组织的界面组织，连通性和交流中的作用有限的理解有限。在该项目中，一种能够同时光学相干性和多光子显微镜的高级集成显微镜，并且光学相干弹性弹力被用来唯一可视化3-D工程皮肤结构中细胞的结构和功能关系，并测量生物力学特性。其次，该项目在3-D中调查并纵向图像是在不同的微型形成底物上工程皮肤构建体的生长。这将提供对角质形成细胞和成纤维细胞上皮肤表皮结的机械影响的基本见解。第三，将研究机械刺激对这些构建体的影响，从而定义了随着时间的推移，变化的刺激如何影响3-D细胞动力学和组织组织。这种刺激效应将提供更相关的培养条件。最后，该项目将在皮肤结构接枝以宿主临床前模型后纵向对细胞和组织反应进行体内形象，从而极大地有助于我们理解工程组织移植物与生物宿主的接口。