Inactivation of Legionella pneumophila harbored by amoebae using a nano-enabled alternative technology: Application and outreach to the Colonias in Texas

使用纳米替代技术灭活阿米巴虫所携带的嗜肺军团菌：在德克萨斯州殖民地的应用和推广

• 批准号: 1805958
• 负责人: Navid Saleh
• 金额: $ 32.79万
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2022-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1805958&HistoricalAwards=false
• 关键词: Inactivation; Legionella; pneumophila; harbored; amoebae

Legionnaires' disease is a deadly form of pneumonia caused by the bacterial pathogen Legionella pneumophila. Several outbreaks of this waterborne disease have occurred in the U.S. since 1976, and new cases have been growing at a high rate. Exposure occurs when contaminated water droplets are inhaled (e.g., during showering) or aspirated. L. pneumophila can be harbored by free-living single-celled organisms (amoebae), which can shield L. pneumophila from traditional disinfection like a 'Trojan horse', thereby increasing risk for the spread of Legionnaires' disease. This project aims to develop a low-cost disinfection technology that can harness low-energy microwave radiation to inactivate L. pneumophila in drinking water. The work includes a major outreach program with the Texas colonias, or self-built communities, which are home to 400,000 low-income people, many of whom lack access to high quality drinking water. Successful development of this technology has the potential to ensure safe drinking water for the colonias and the Nation. The goal of the proposed irradiation-based technology is to generate interfacial heat and reactive oxygen species (ROS) to inactivate L. pneumophila harbored by amoebae. Amoebae will be lysed via interfacial heating, and the released L. pneumophila will be inactivated by heat and ROS. The proposed research will deploy fundamental nanoscience to develop a point-of-use (POU) and community-scale water treatment technology. The specific technical objectives of the proposed research plan are to (i) synthesize, characterize, and test MW-harnessing nanomaterials, (ii) understand the mechanisms of microbial inactivation by this MW-based nanotechnology, and (iii) engineer two devices containing immobilized nanomaterials to effectively inactivate L. pneumophila harbored by amoebae. The research will capitalize on the scientific advantages of novel MW spectral conversion to provide safe drinking water to low-income residents of the Texas colonias and beyond. Outreach activities will occur in elementary schools and colonias located in three school districts (two in El Paso County and one in Hidalgo County, both of which have high concentrations of colonias). Hands-on educational activities related to the research will be conducted in the schools, and water sampling and household surveys will be conducted in the colonias to gather information on water quality, water use, water-related health issues, and socioeconomic conditions. The outreach is unique because the information it yields will be utilized in the device design, thereby enabling the development of a socially-embedded technology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

军团病是一种致命的肺炎，由细菌病原体嗜肺军团菌引起。自 1976 年以来，美国已多次爆发这种水传播疾病，并且新病例一直在高速增长。 当吸入（例如淋浴时）或吸入受污染的水滴时，就会发生暴露。嗜肺军团菌可以被自由生活的单细胞生物（阿米巴原虫）所携带，这可以像“特洛伊木马”一样保护嗜肺军团菌免受传统消毒的影响，从而增加军团病传播的风险。该项目旨在开发一种低成本消毒技术，利用低能微波辐射灭活饮用水中的嗜肺军团菌。这项工作包括一项针对德克萨斯州殖民地或自建社区的重大外展计划，这些社区居住着 40 万低收入人群，其中许多人无法获得高质量的饮用水。这项技术的成功开发有可能确保殖民地和国家的安全饮用水。所提出的基于辐射的技术的目标是产生界面热和活性氧（ROS）以灭活阿米巴携带的嗜肺军团菌。变形虫将通过界面加热被裂解，释放的嗜肺军团菌将被热和活性氧灭活。拟议的研究将利用基础纳米科学来开发使用点（POU）和社区规模的水处理技术。 拟议研究计划的具体技术目标是（i）合成、表征和测试MW利用纳米材料，（ii）了解这种基于MW的纳米技术灭活微生物的机制，以及（iii）设计两种包含固定化纳米材料的装置纳米材料可有效灭活阿米巴携带的嗜肺军团菌。该研究将利用新型微波光谱转换的科学优势，为德克萨斯州殖民地及其他地区的低收入居民提供安全的饮用水。外展活动将在位于三个学区的小学和殖民地进行（两所位于埃尔帕索县，一所位于伊达尔戈县，这两个学区都是殖民地高度集中的地区）。与研究相关的实践教育活动将在学校进行，并将在殖民地进行水采样和家庭调查，以收集有关水质、用水、与水有关的健康问题和社会经济状况的信息。此次推广活动是独一无二的，因为它产生的信息将用于设备设计，从而促进社会嵌入式技术的开发。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力优势和更广泛的评估进行评估，被认为值得支持。影响审查标准。

项目成果

Compromised Water Quality in Colonias of Nueces County, TX: A Vicious Cycle

德克萨斯州纽埃西斯县科洛尼亚斯的水质受损：恶性循环

• 发表时间: 2019-10
• 期刊: University of North Carolina Chapel Hill Water & Health Conference
• 作者: Rowles III, L. S.;Hossain, A. H.;Lawler, D. F.;Kirisits, M. J.;Araiza, I.;Saleh, N. B.
• 通讯作者: Saleh, N. B.

Legionella pneumophila inactivation potency of silver nanoparticles and ionic silver and copper enhanced with microwave radiation

微波辐射增强银纳米颗粒和离子银和铜的嗜肺军团菌灭活效力

• 发表时间: 2019-11
• 期刊: 8th Sustainable Nanotechnology Organization Conference
• 作者: Ayres, C.;Saleh, N. B.;Kirisits, M. J.;Lawler, D. F.
• 通讯作者: Lawler, D. F.

Inactivation of Legionella Pneumophila-Harbored by Amoebae Using a Nano-Enabled Alternative Technology

使用纳米替代技术灭活阿米巴携带的嗜肺军团菌

• 发表时间: 2019-11
• 期刊: and SSSA Annual Meeting
• 作者: Saleh, N. B.;Kirisits, M. J.;Ayres, C.
• 通讯作者: Ayres, C.

Seasonal contamination of well-water in flood-prone colonias and other unincorporated U.S. communities

易发洪水的殖民地和其他美国非建制社区的井水季节性污染

• DOI: 10.1016/j.scitotenv.2020.140111
• 发表时间: 2020-10
• 期刊: Science of The Total Environment
• 影响因子: 9.8
• 作者: Rowles III, Lewis Stetson;Hossain, Areeb I.;Ramirez, Isac;Durst, Noah J.;Ward, Peter M.;Kirisits, Mary Jo;Araiza, Isabel;Lawler, Desmond F.;Saleh, Navid B.
• 通讯作者: Saleh, Navid B.

Synergy between Microwave Radiation and Silver Ions or Nanoparticles for Inactivating Legionella pneumophila

微波辐射与银离子或纳米颗粒之间的协同作用灭活嗜肺军团菌

• DOI: 10.1021/acs.estlett.1c00371
• 发表时间: 2021-07-13
• 期刊: Environmental Science & Technology Letters
• 影响因子: 10.9
• 作者: Craig Ayres;D. Lawler;M. Kirisits;Navid B. Saleh
• 通讯作者: Navid B. Saleh