Probing Contrast Mechanisms of Super-resolution Atomic Force Microscopy for Imaging Multifunctional Self-assembled Monolayers

超分辨率原子力显微镜成像多功能自组装单层膜的对比机制探索

• 批准号: 1808213
• 负责人: Tao Ye
• 金额: $ 48.5万
• 依托单位: University of California - Merced
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1808213&HistoricalAwards=false
• 关键词: Probing; Contrast; Mechanisms; Super; resolution

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry, Professors Tao Ye and Ashlie Martini at University of California, Merced, are combining experimental and computational approaches to study how molecules interact at the atomic level and how this interaction affects the resolution of an imaging tool -atomic force microscope. They hope their findings could improve the ability to image complex, technologically relevant surfaces such as biosensors and devices under practical operating conditions. Given the importance of biosensors and devices in wearable technologies, personalized medicine and national security, the advances made through this project could potentially offer long-term economic and societal benefits. The research goals of the project are complemented by education/outreach objectives that leverage the diverse student population at UC Merced. Professors Ye and Martini plan to actively recruit and train talented graduate and undergraduate students from underrepresented groups. The research team is also engaging with pre-college audiences through a variety of mechanisms. A key goal of the research project is to understand the interaction forces between a small number of atoms at the very end of the atomic force microscope (AFM) tip, i.e., the tip apex, and a small number of atoms on the surface; these interactions ultimately determine the subnanometer scale contrast of in situ atomic force microscopy. Professors Ye and Martini are elucidating the nature of the chemical forces responsible for subnanometer-scale contrast by combining non-contact imaging of nanoscale chemical patterns with a chemically modified AFM tip apex and state-of-the-art molecular dynamics simulations of AFM imaging. Moreover, the team is applying the knowledge to obtain more reproducible super-resolution mapping of the molecular components of DNA-functionalized self-assembled monolayers that have been widely used in electrochemical DNA sensors. The proposed new experimental and computational approaches are filling a critical knowledge gap in the image contrast mechanisms of subnanometer resolution AFM and allow it to become a more reproducible and broadly applicable surface chemical imaging tool. The new advances may be applicable to a variety of surfaces, including self-assembled monolayer-based biosensors, microarrays, and supported lipid bilayers, and can impact fields from biotechnology to life sciences.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学测量和化学成像计划的支持下，加利福尼亚大学默塞德大学的陶叶和阿什利·马蒂尼教授正在结合实验和计算方法，以研究分子在原子水平上的相互作用以及这种相互作用如何影响成像工具的分辨率的分辨率。他们希望他们的发现能够提高成像复杂，技术相关的表面，例如生物传感器和设备在实际操作条件下。鉴于生物传感器和设备在可穿戴技术，个性化医学和国家安全方面的重要性，通过该项目取得的进步可能会带来长期的经济和社会利益。该项目的研究目标得到了教育/外展目标的补充，这些目标利用了UC Merced的不同学生群体。 Ye和Martini教授计划积极招募和培训来自代表性不足的团体的才华横溢的研究生和本科生。研究团队还通过各种机制与大学前观众互动。研究项目的一个关键目的是了解原子力显微镜（AFM）尖端末端的少量原子之间的相互作用力，即尖端顶点和表面上的少量原子。这些相互作用最终决定了原位原子力显微镜的亚纳光尺度对比。 Ye和Martini教授通过将纳米级化学模式的非接触式成像与化学改性的AFM TIP顶点和AFM成像的最先进的分子动力学模拟相结合，从而阐明了负责亚光尺度对比度的化学力性质。此外，该团队正在应用知识，以获取更可重现的DNA官能化自组装单层的分子成分的超级分辨率映射，这些单层已被广泛用于电化学DNA传感器。拟议的新实验和计算方法正在填补亚纳光分辨率AFM的图像对比机制中的关键知识差距，并允许它成为一种更可再现和广泛适用的表面化学成像工具。 新进步可能适用于各种表面，包括基于单层的生物传感器，微阵列和支持的脂质双层型，可能会影响从生物技术到生命科学的领域。该奖项反映了NSF的法定任务，并通过评估基金会的智力效果，并被认为是值得通过评估的支持。

项目成果

Nanoscale Friction of Hydrophilic and Hydrophobic Self-Assembled Monolayers in Water

• DOI: 10.1007/s11249-020-01301-0
• 发表时间: 2020-04
• 期刊: Tribology Letters
• 影响因子: 3.2
• 作者: Quanpeng Yang;Warren A. Nanney;Xiaoli Hu;T. Ye;A. Martini

Single molecule insights into interfacial molecular recognition for model electrochemical DNA biosensors

• DOI: 10.1016/j.coelec.2023.101348
• 发表时间: 2023-07-31
• 期刊: CURRENT OPINION IN ELECTROCHEMISTRY
• 影响因子: 8.5
• 作者: Gu，Qufei;Petrek，Zachary;Ye，Tao
• 通讯作者: Ye，Tao

Simulation of Subnanometer Contrast in Dynamic Atomic Force Microscopy of Hydrophilic Alkanethiol Self-Assembled Monolayers in Water

亲水性烷硫醇在水中自组装单分子层的动态原子力显微镜亚纳米对比度模拟

• DOI: 10.1021/acs.langmuir.9b03655
• 发表时间: 2020
• 期刊: Langmuir
• 影响因子: 3.9
• 作者: Hu, Xiaoli;Yang, Quanpeng;Ye, Tao;Martini, Ashlie
• 通讯作者: Martini, Ashlie

Transfer of Thiolated DNA Staples from DNA Origami Nanostructures to Self-Assembled Monolayer-Passivated Gold Surfaces: Implications for Interfacial Molecular Recognition

• DOI: 10.1021/acsanm.1c01685
• 发表时间: 2021-07
• 期刊: ACS Applied Nano Materials
• 影响因子: 5.9
• 作者: Qufei Gu;Yehan Zhang;Huan H. Cao;Songtao Ye;T. Ye

Toward a Quantitative Relationship between Nanoscale Spatial Organization and Hybridization Kinetics of Surface Immobilized Hairpin DNA Probes

表面固定化发夹 DNA 探针纳米级空间组织与杂交动力学之间的定量关系

• DOI: 10.1021/acssensors.0c01278
• 发表时间: 2021
• 期刊: ACS Sensors
• 影响因子: 8.9
• 作者: Gu, Qufei;Cao, Huan H.;Zhang, Yehan;Wang, Haiyang;Petrek, Zachary J.;Shi, Fukun;Josephs, Eric A.;Ye, Tao
• 通讯作者: Ye, Tao