Directing and Probing DNA Origami Self-Assembly on Dynamic Surfaces

指导和探测动态表面上的 DNA 折纸自组装

• 批准号: 1410199
• 负责人: Tao Ye
• 金额: $ 51万
• 依托单位: University of California - Merced
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-15 至 2018-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1410199&HistoricalAwards=false
• 关键词: Directing; Probing; DNA; Origami; Self

Non-technical: This award by the Biomaterials Program in the Division of Materials Research to University of California, Merced is to understand how DNA folds to make a variety of large scale structures. Nature has developed the ability to assemble biomolecules into complex biological structures which allow the molecular components to work in concert to perform the elaborate functions of life. A long-standing endeavor is to develop methods that allow molecular building blocks to self-organize into higher order structures. The DNA origami method folds a long, single-stranded DNA molecule into nanoscale shapes using short oligonucleotide strands with complementary sequences. By developing high-resolution microscopy techniques that can capture snapshots of individual DNA origami structures as they form, the PI will begin to understand the mechanism of the self assembly process. The findings of this research may enable the self-assembly of complex structures that can perform novel functions such as sensing and nanoelectronic and photonic circuits.The project will provide graduate students and postdoc researchers with interdisciplinary training that is needed to address high-profile challenges in biomolecular materials. It will also bring new research opportunities to undergraduate students at UC Merced, a large fraction of whom are under-represented minorities. Working with the local school district, the PI will host lab tours and develop nanoscience demonstrations.Technical:The project seeks to understand the self-assembly pathways of DNA origami and introduce precise surface interactions to regulate the self-assembly process. The ensemble spectroscopy and ex-situ microscopy techniques commonly used provide limited information concerning the intermediate structures. The PI will fold DNA origami tiles on dynamic self-assembled monolayer (SAM) surfaces, which can rapidly trap the intermediate structures for high resolution AFM imaging. With detailed structural information of the folding intermediates, the PI will begin to reconstruct and elucidate the folding pathways. Building upon novel surface chemistry developed in the lab, the PI will enable individual oligonucleotide molecules to nucleate the formation of origami tiles of specific size, shape and internal arrangement on the SAM surface. If successful, the study will significantly advance the understanding of the self-assembly process of DNA origami nanostructures and potentially allow the formation of much larger structures with more sophisticated functions. The project will prepare graduate students and a postdoctoral scholar to tackle high-profile challenges in biomolecular materials, a field demanding highly interdisciplinary solutions.

非技术性：生物材料计划在加利福尼亚大学材料研究部授予的奖项，默塞德是要了解DNA如何折叠以制造各种大型结构。 大自然已经发展了将生物分子组装成复杂的生物结构的能力，这些生物分子使分子成分可以协同起作用，以执行详尽的生命功能。一项长期的努力是开发使分子构建块自我组织成高阶结构的方法。 DNA折纸方法使用具有互补序列的短寡核苷酸链将长的单链DNA分子折叠成纳米级形状。 通过开发高分辨率显微镜技术，可以捕获单个DNA折纸结构形成时的快照，PI将开始理解自组装过程的机制。 这项研究的结果可能使复杂结构的自组装能够执行新的功能，例如传感和纳米电机和光子电路。该项目将为研究生和博士后研究人员提供跨学科培训，以应对生物分子材料中的高调挑战所需的跨学科培训。它还将为UC Merced的本科生带来新的研究机会，UC Merced的本科生中有很大一部分是代表性不足的少数群体。 PI与当地学区合作，将举办实验室旅行并开发纳米科学示范。技术：该项目旨在了解DNA折纸的自组装途径，并引入精确的表面相互作用以调节自组装过程。 通常使用的集合光谱和前坐标显微镜技术提供了有限的有关中间结构的信息。 PI将在动态自组装单层（SAM）表面上折叠DNA折纸瓷砖，该表面可以快速捕获高分辨率AFM成像的中间结构。 借助折叠中间体的详细结构信息，PI将开始重建并阐明折叠途径。在实验室中开发的新型表面化学基础上，PI将使单个寡核苷酸分子能够对SAM表面上特异性，形状和内部排列的折纸瓷砖形成。如果成功，这项研究将显着提高人们对DNA折纸纳米结构的自组装过程的理解，并有可能允许形成具有更复杂功能的更大结构。 该项目将为研究生和博士后学者做好准备，以应对生物分子材料中的备受瞩目的挑战，这是一个要求高度跨学科解决方案的现场。

项目成果

Single Molecule Profiling of Molecular Recognition at a Model Electrochemical Biosensor

• DOI: 10.1021/jacs.8b07325
• 发表时间: 2018-10-31
• 期刊: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
• 影响因子: 15
• 作者: Gu, Qufei;Nanney, Warren;Ye, Tao
• 通讯作者: Ye, Tao

Seeding the Self-Assembly of DNA Origamis at Surfaces

在表面播种 DNA 折纸的自组装

• DOI: 10.1021/acsnano.9b09348
• 发表时间: 2020
• 期刊: ACS Nano
• 影响因子: 17.1
• 作者: Cao, Huan H.;Abel, Gary R.;Gu, Qufei;Gueorguieva, Gloria-Alexandra V.;Zhang, Yehan;Nanney, Warren A.;Provencio, Eric T.;Ye, Tao
• 通讯作者: Ye, Tao