EAGER: Dynamic Surface Interactions for Single Molecule Imaging of Biochemical Reactions

EAGER：生化反应单分子成像的动态表面相互作用

• 批准号: 1361066
• 负责人: Tao Ye
• 金额: $ 11万
• 依托单位: University of California - Merced
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-12-01 至 2014-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1361066&HistoricalAwards=false
• 关键词: EAGER; Dynamic; Surface; Interactions; Single

In this EAGER project funded by the Chemical Measurement and Imaging Program in the Division of Chemistry, Professor Tao Ye at University of California-Merced and his group will use atomic force microscopy (AFM) to visualize the conformational changes and biochemical reactions of DNA molecules that are anchored to electroactive surfaces, i.e., surfaces that dynamically change their affinity with molecular adsorbates under electrochemical control. Such novel dynamic surfaces will help address a long-standing challenge in AFM visualization of biochemical reactions: if the biomacromolecules are pinned down, which is needed for high resolution AFM, the interactions with the surface perturb the reaction. The dynamic surfaces will expand the repertoire of surfaces and reactions that can be probed by single molecule AFM imaging, significantly reduce the measurement artifacts, and improve the imaging resolution. Therefore, the project will further our understanding of the reactions of biomacromolecules at the nanoscale and single molecule level. The project will train graduate students at the cutting edge of nanoscale measurement and imaging. The proposed research will also provide research opportunities to undergraduate students at UC Merced, many of whom are underrepresented minorities.

在化学划分中由化学测量和成像计划资助的渴望的项目中，加利福尼亚大学 - 摩西大学的陶叶教授及其小组将使用原子力显微镜（AFM）可视化构象变化和生化反应，而DNA分子的生物化学反应是固定在电动表面的构造，即对电动性构成的构造，即动态变化，这些反应是在电位变化的。电化学控制。 这种新型的动态表面将有助于解决生物化学反应的AFM可视化的长期挑战：如果将生物分子固定下来，这是高分辨率AFM所需的，则与表面扰动反应相互作用。动态表面将扩大表面和反应的曲目，可以通过单分子AFM成像探测，从而显着减少测量伪像并改善成像分辨率。因此，该项目将进一步理解纳米级和单分子水平上生物大分子的反应。 该项目将在纳米级测量和成像的最前沿培训研究生。 拟议的研究还将为UC Merced的本科生提供研究机会，UC默塞德（UC Merced）中的许多人代表性不足。