Linking genetics and mineralization of iron-oxidizing microbes at midocean ridges

将洋中脊铁氧化微生物的遗传学和矿化联系起来

• 批准号: 0752594
• 负责人: Katrina Edwards
• 金额: $ 4万
• 依托单位: University of Southern California
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-15 至 2009-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0752594&HistoricalAwards=false
• 关键词: Linking; genetics; mineralization; iron; oxidizing

The objective of the original and current research is to study the genetic basis of neutrophilic microbial iron oxidation to elucidate its relation to iron oxyhydroxide biomineralization and develop a means to monitor biological iron oxidation in the environment. This work involves identifying components of the iron oxidation electron transport pathways using genomic analysis and protein isolation and characterization techniques. Iron-oxidizing proteins will be located in cells from cultures using antibody labeling and electron microscopy. The data will be analyzed to determine the spatial and functional relationships between enzymatic iron oxidation, extracellular polymer formation, and iron oxyhydroxide mineralization. The primary results anticipated include the identification of an iron oxidase enzyme and microscopic proof that this enzyme deposits oxidized iron on polymers that are extruded outside the cell to form a mineralized biofilm matrix. Such results would represent a major breakthrough in understanding the physiology of iron-oxidizing bacteria (FeOB).The broader impacts of this project will be its advancement of the career of a promising young female investigator. It will also have a significant beenfit to the broader scientific community. The role of iron oxidizing microbes in the formation of ubiquitous deposits is in need of greater study.

原始研究和当前研究的目的是研究嗜中性微生物铁氧化的遗传基础，以阐明其与氧化氧化铁生物矿化的关系，并开发一种在环境中监测生物铁氧化的手段。这项工作涉及使用基因组分析以及蛋白质分离和表征技术鉴定铁氧化电子传输途径的组成部分。铁氧化蛋白将使用抗体标记和电子显微镜从培养物中定位在细胞中。将分析数据，以确定酶促氧化，细胞外聚合物形成和羟基氧化氧化铁矿化之间的空间和功能关系。预期的主要结果包括鉴定氧化酶的酶和微观证明，该酶沉积在细胞外挤出的聚合物上氧化铁以形成矿化的生物膜基质。这种结果将代表理解铁氧化细菌（FEOB）生理学的重大突破。该项目的更广泛影响将是其对有前途的年轻女性调查员的职业发展的进步。 这也将对更广泛的科学界有很大的支持。铁氧化微生物在形成无处不在的沉积物中的作用需要更大的研究。