Linking genetics and mineralization of iron-oxidizing microbes at midocean ridges

将洋中脊铁氧化微生物的遗传学和矿化联系起来

基本信息

批准号：
0752594
负责人：
Katrina Edwards
金额：
$ 4万
依托单位：
University of Southern California
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2008
资助国家：
美国
起止时间：
2008-02-15 至 2009-01-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0752594&HistoricalAwards=false
关键词：
Linking genetics mineralization iron oxidizing

项目摘要

The objective of the original and current research is to study the genetic basis of neutrophilic microbial iron oxidation to elucidate its relation to iron oxyhydroxide biomineralization and develop a means to monitor biological iron oxidation in the environment. This work involves identifying components of the iron oxidation electron transport pathways using genomic analysis and protein isolation and characterization techniques. Iron-oxidizing proteins will be located in cells from cultures using antibody labeling and electron microscopy. The data will be analyzed to determine the spatial and functional relationships between enzymatic iron oxidation, extracellular polymer formation, and iron oxyhydroxide mineralization. The primary results anticipated include the identification of an iron oxidase enzyme and microscopic proof that this enzyme deposits oxidized iron on polymers that are extruded outside the cell to form a mineralized biofilm matrix. Such results would represent a major breakthrough in understanding the physiology of iron-oxidizing bacteria (FeOB).The broader impacts of this project will be its advancement of the career of a promising young female investigator. It will also have a significant beenfit to the broader scientific community. The role of iron oxidizing microbes in the formation of ubiquitous deposits is in need of greater study.

最初和当前研究的目的是研究中性粒细胞微生物铁氧化的遗传基础，以阐明其与羟基氧化铁生物矿化的关系，并开发一种监测环境中生物铁氧化的方法。这项工作涉及使用基因组分析以及蛋白质分离和表征技术来识别铁氧化电子传递途径的成分。使用抗体标记和电子显微镜可以定位培养物细胞中的铁氧化蛋白。对数据进行分析以确定酶促铁氧化、细胞外聚合物形成和羟基氧化铁矿化之间的空间和功能关系。预期的主要结果包括铁氧化酶的鉴定以及该酶将氧化铁沉积在聚合物上的微观证据，这些聚合物被挤出到细胞外形成矿化生物膜基质。这些结果将代表着理解铁氧化细菌 (FeOB) 生理学的重大突破。该项目的更广泛影响将是促进一位有前途的年轻女性研究员的职业生涯。它还将对更广泛的科学界产生重大影响。铁氧化微生物在普遍存在的沉积物形成中的作用需要进一步研究。