Collaborative Research: Completing single- and cross-hole hydrgeologic and microbial experiments: Juan de Fuca Flank

合作研究：完成单孔和跨孔水文地质和微生物实验：Juan de Fuca Flank

• 批准号: 1260697
• 负责人: Katrina Edwards
• 金额: $ 7.58万
• 依托单位: University of Southern California
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1260697&HistoricalAwards=false
• 关键词: Collaborative; Research; Completing; single; cross

In 2010, IODP exp. 327 launched series of experiments based on instrumentation placed in 6 boreholes (Holes 1026B, 1027C, 1301A, 1301B, 1362A, and 1362B) on and near the Juan de Fuca Ridge (JDFR). The experiments were designed to examine hydrological and biological processes in basaltic ocean crust. Major issues to be addressed include overall permeability, the magnitudes, velocity and directions of fluid, solute,and heat transport,constraints on the fluid storage properties of crust, determining how fluid reservoirs respond to seismic perturbations, and investigating the variability, diversity, and metabolism of resident microbial populations. This project will recover sensors and samplers in the instrumented boreholes(CORKs),access data and samples, collect biological incubators placed at depth within the boreholes, and seal the CORKs. All of these activities are needed for the completion of the JDFR experimentsOnce the data and samples are retrieved, the PI's will develop new numerical models for the hydrology (permeability, thickness and extent of permeable zones, connectivity, direction of flow, and anisotropy) of ocean crust based on the results of flow tests. T sensors will enable thermal structure to be determined in conjunction with flow parameters. DNA will be analyzed via ?16S ribosomal RNA (rRNA) genes as well as select full-length Sanger-style sequencing and gene fingerprinting techniques.? The PI's will look at diversity, shared species, emergence and disappearance of groups. Metagenomic work will be done on some samples.Broader Impacts include training of 4 shipboard educators, with an emphasis on recruiting underrepresented groups for these positions, teacher workshops, and special training for undergraduate and graduate students and post-docs. Research team includes grads, undergrads, and post-docs.

在2010年，IODP Exp。 327个基于在Juan de Fuca Ridge（JDFR）上及其附近的仪器（孔1026b，1027c，1301b，1362a和1362b）放置的仪器（孔1026b，1027c，1301a，1301b，1362a和1362b）发起了一系列实验。该实验旨在检查玄武岩海皮中的水文和生物过程。要解决的主要问题包括整体渗透率，量，速度和流体，溶质和热传输的方向，对地壳的流体储存特性的约束，确定液体储层如何响应地震扰动以及研究可变性，多样性，以及多样性，以及多样性，以及多样性，以及多样性，以及多样性，以及多样性，以及多样性，以及多样性的扰动。常驻微生物种群的代谢。该项目将在仪器井（软木塞）中恢复传感器和采样器，访问数据和样品，收集位于钻孔深处的生物孵化器，然后密封软木塞。所有这些活动都是为了完成JDFR实验所需的所有这些活动，并检索了样品，PI将开发用于水文学的新数值模型（可渗透性区域的渗透率，厚度和程度，连通性，流动方向和各向异性）根据流程测试的结果，海壳。 T传感器将使热结构与流参数结合确定。 DNA将通过16S核糖体RNA（RRNA）基因进行分析，以及选择全长的Sanger式测序和基因指纹技术。 PI将研究群体的多样性，共享物种，出现和消失。宏基因组学的工作将在某些样本上进行。Broader的影响包括对4名船板教育者的培训，重点是招募这些职位的代表性不足的小组，教师讲习班以及针对本科生和研究生和研究生和后培训的特殊培训。研究团队包括毕业生，本科生和毕业后。