Development and Testing of Materials Methods for In-situ Incubations and Subsurface Microbial Observatories at North Pond

北池塘原位培养和地下微生物观测站材料方法的开发和测试

• 批准号: 0625980
• 负责人: Katrina Edwards
• 金额: $ 24.43万
• 依托单位: Woods Hole Oceanographic Institution
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0625980&HistoricalAwards=false
• 关键词: Development; Testing; Materials; Methods; situ

Deep biosphere and subseafloor hydrosphere studies are critical focus areas for the Integrated Ocean Drilling Program (IODP). In this project, researchers will develop and test new instrumentation for the incubation of microbes in boreholes that may be drilled and CORKed at North Pond, on the western flank of the Mid-Atlantic Ridge at 22 degrees 45 minutes north, under the auspices of a pending IODP project. This project will also extend analysis techniques (Flourescently-Labeled Embedded Core or FLEC) that permit careful study of the spatial relationships ('life locations') among microbes for use in various microbial studies in the deep marine environment, including on the incubators to be developed in this project. FLEC, combined with established ribosomal RNA detection methods (CARD and FISH), will provide significantly more information about the microbial systems, including community structure and microbial associations at a fine scale. Broader impacts include training of graduate and undergraduate students, advancement of IODP science and building on ODP science, hosting of a workshop, and educational outreach to teacher groups.

深层生物圈和海底水圈研究是综合海洋钻探计划（IODP）的关键重点领域。 在这个项目中，研究人员将开发和测试用于在钻孔中孵化微生物的新仪器，这些仪器可以在大西洋中脊西侧北纬 22 度 45 分钟的北池塘 (North Pond) 钻探和塞住，由待定的 IODP 项目。 该项目还将扩展分析技术（荧光标记嵌入式核心或 FLEC），允许仔细研究微生物之间的空间关系（“生命位置”），用于深海环境中的各种微生物研究，包括在培养箱上进行研究。在这个项目中开发的。 FLEC 与已建立的核糖体 RNA 检测方法（CARD 和 FISH）相结合，将提供更多有关微生物系统的信息，包括精细尺度的群落结构和微生物关联。 更广泛的影响包括培训研究生和本科生、推进 IODP 科学并以 ODP 科学为基础、举办研讨会以及对教师团体进行教育推广。