SGER: A novel approach to a biocompatible antibody-antigen recognition system using antigen imprinted polymers

SGER：使用抗原印迹聚合物构建生物相容性抗体-抗原识别系统的新方法

• 批准号: 0640778
• 负责人: Peter Kofinas
• 金额: --
• 依托单位: University of Maryland, College Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-15 至 2008-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0640778&HistoricalAwards=false
• 关键词: SGER; novel; approach; biocompatible; antibody

Abstract Proposal Title: SGER A novel approach to a biocompatible antibody-antigen recognition system using antigen imprinted polymers. Proposal Number: CTS-0640778 Principal Investigator: Peter Kofinas, Institution: University of Maryland College ParkThe intellectual merit and aim of this exploratory research is to imprint a tumor specific antigen (Ag) into a polymeric scaffold using techniques already developed in the PI's laboratory. The resulting molecularly imprinted polymers (MIPs) would contain antigen-shaped cavities, which would elicit specific immune system responses trigering the production of antibodies. The hypothesis for this work is that the imprinting process will create cavities within the polymer matrix that are complementary to the shape and size of the targeted antibody, resulting in the specific and preferential specific binding of that antibody to the Ag-MIP. The recognition ability of the Ag-MIP will be determined by testing the affinity for antibodies of different isotypes in competitive and non-competitive affinity experiments.The broader impacts of the proposed research relate to development of novel techonolgies with the unparalleled recognition capability of antibody-antigen recognition present in the human immune system, which could have profound effects within the field of medicine and biotechnology. Such technologies would pave the way for the creation of revolutionary tech- niques to detect and treat diseases. Ag-MIPs can be applied to affinity matrices for applications in separation and purification, biosensor technology and diagnostics. Underlying all immunological approaches to cancer is the idea that tumor cells must differ from normal cells, and that these differences must be detectable by the immune system. Tumor immunologists have tried to exploit this by using antibodies produced in another species to distinguish between tumor and normal cells; this difference can then be exploited for diagnostic, prognostic, or therapeutic purposes. The polymer developed in this research imprinted against the MZ2-E tumor-specific antigen could elicit an immune response (antibody production), when injected to cancer patients, which could help lyse (kill) the cancerous tumor. Artifical antibodies made from MIPs can also be used in diagnostics for the detection of antigens and antibodies in blood samples, for the profiling of sera to discover new disease markers, and for environment and food monitoring. In biosensor technology artificial antibodies can be used to profile protein expression and to detect specific protein-protein interactions. The education activities to be undertaken in this work include graduate student mentoring.

摘要提案标题：SGER 一种使用抗原印迹聚合物构建生物相容性抗体-抗原识别系统的新方法。提案编号：CTS-0640778 首席研究员：Peter Kofinas，机构：马里兰大学学院公园分校 这项探索性研究的智力价值和目的是利用 PI 实验室已开发的技术将肿瘤特异性抗原 (Ag) 印迹到聚合物支架中。由此产生的分子印迹聚合物（MIP）将包含抗原形状的空腔，这将引发特定的免疫系统反应，从而触发抗体的产生。这项工作的假设是，印记过程将在聚合物基质内产生与目标抗体的形状和大小互补的空腔，从而导致该抗体与 Ag-MIP 特异性且优先的特异性结合。 Ag-MIP 的识别能力将通过在竞争性和非竞争性亲和力实验中测试不同同种型抗体的亲和力来确定。本研究的更广泛影响涉及到具有抗体无与伦比的识别能力的新技术的开发。人类免疫系统中存在抗原识别，这可能在医学和生物技术领域产生深远的影响。这些技术将为创造检测和治疗疾病的革命性技术铺平道路。 Ag-MIP 可应用于亲和基质，用于分离和纯化、生物传感器技术和诊断。所有癌症免疫学方法的基础是肿瘤细胞必须与正常细胞不同，并且这些差异必须能够被免疫系统检测到。肿瘤免疫学家试图利用这一点，使用其他物种产生的抗体来区分肿瘤细胞和正常细胞。然后可以利用这种差异用于诊断、预后或治疗目的。这项研究中开发的针对 MZ2-E 肿瘤特异性抗原印迹的聚合物在注射到癌症患者体内时可以引发免疫反应（抗体产生），这可以帮助溶解（杀死）癌性肿瘤。由 MIP 制成的人工抗体还可用于诊断，以检测血液样本中的抗原和抗体、分析血清以发现新的疾病标记物，以及用于环境和食品监测。在生物传感器技术中，人工抗体可用于分析蛋白质表达并检测特定的蛋白质-蛋白质相互作用。这项工作中要进行的教育活动包括研究生指导。