Comparative cDNA Sequencing in Radish (Raphanus), a Crop, Weed, and Model System in Ecology and Evolution

萝卜（Raphanus）、一种作物、杂草以及生态学和进化模型系统的比较 cDNA 测序

• 批准号: 0638591
• 负责人: Jeffrey Conner
• 金额: --
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-12-01 至 2010-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0638591&HistoricalAwards=false
• 关键词: Comparative; cDNA; Sequencing; Radish; Raphanus

There are two species in the plant genus Raphanus: R. sativus is the edible radish and R. raphanistrum is the wild species. The wild species is important because it is the likely ancestor of the crop, it is an increasingly serious crop weed worldwide, and because hybrids between the wild and crop radish have become an invasive species of natural land in California. Radish is also a model system in ecology and evolution, especially for studies of plant-animal interactions and natural selection. Despite its importance as a crop, weed, and study species, almost no gene sequence data is available for radish, severely limiting future progress in understanding the biology of radish. This project will produce sequences of thousands of genes from both the crop and wild radish species and generate hundreds of genetic markers. These sequences and markers will enable the next generation of discoveries, including the genetic basis of: crop domestication, weed and invasive species evolution, adaptation to pollinators, herbivores, and global change. Comparisons of the gene sequences generated to existing sequences from other species in the same family (the crop genus Brassica and the model plant Arabidopsis) will advance our understanding of how gene sequences evolve. Access to project outcomesThe sequences, markers, and clones produced will be made freely available to the broader research community via GenBank, the Arabidopsis Biological Resource Center (ABRC: http://www.biosci.ohio-state.edu/pcmb/Facilities/abrc/abrchome.htm), Plant Genome Database (PlantGDB: http://www.biosci.ohio-state.edu/pcmb/Facilities/abrc/abrchome.htm), the Arabidopsis Information Resource (TAIR: http://www.arabidopsis.org/) and websites at the Institute for Genomic Research (TIGR: http://www.tigr.org/) and Michigan State University (MSU: http://www.kbs.msu.edu/Faculty/Conner/Index.htm). The MSU website will feature collaborative content creation (wiki) to facilitate the exchange of ideas and data among the worldwide radish research community.

植物属植物中有两个物种：sativus是可食用的萝卜，而R. raphanistrum是野生物种。野生物种很重要，因为它可能是农作物的祖先，它是全球越来越严重的农作物杂草，并且因为野生和农作物萝卜之间的杂种已成为加利福尼亚州自然土地的入侵物种。 萝卜也是生态学和进化的模型系统，尤其是用于研究植物 - 动物相互作用和自然选择的研究。 尽管它作为农作物，杂草和研究物种的重要性，但几乎没有基因序列数据可用于萝卜，严重限制了未来在理解萝卜生物学方面的进展。 该项目将产生来自农作物和野生萝卜物种的数千个基因的序列，并产生数百个遗传标记。 这些序列和标记将使下一代发现，包括：农作物驯化，杂草和入侵物种进化，对传粉媒介，食草动物和全球变化的适应。 比较同一家族中其他物种的现有序列（农作物属和模型植物拟南芥）生成的基因序列将提高我们对基因序列如何发展的理解。 可以通过GenBank自由使用GenBank，拟南芥生物资源中心（ABRC：http：//wwwwwww.biosci.ohio-state.edu/pcmb/pcmb/faccmb/faciolities/abrc/abrc/abrchome.htm），访问更广泛的研究社区的项目访问序列，标记和克隆的访问权限。 http://www.biosci.ohio-state.edu/pcmb/facilities/abrc/abrchome.htm），拟南芥信息资源（TAIR：http：//www.arabidopsis.org/大学（MSU：http：//www.kbs.msu.edu/faculty/conner/index.htm）。 MSU网站将采用协作内容创建（WIKI），以促进全球萝卜研究社区之间的思想和数据交换。