椎间盘细胞及分子生物力学应答机制的影像学研究

Low back pain is the most common clinical problem in adult population, and the second leading cause of disability worldwide. As a international medical and social problem, low back pain is closely associated with intervertebral disc degeneration (IVDD). Therapeutic intervention has been invalid because the abnormal biomechanical response that causes IVDD has not been hampered. Studies suggest that abnormal mechanical load acts as a trigger in IVDD, and the cellular and molecular biomechanical response results in IVDD. So non-invasive in vivo imaging of cellular and molecular biomechanical response in intervertebral disc, which will provide a reliable basis for early treatments, is a scientific problem to be solved..This project is about the imaging of the cellular and molecular biomechanical response in intervertebral disc under abnormal mechanical load. The contents are as follows: 1.nutrition: imaging of nutrient source (porosity and capillary) , imaging of transportion of nutrition - metabolite nutrient (glucose, oxygen, lactic acid); 2.cellular mechanobiology: imaging of integrin and Ca2+ ; 3. cellular reactions: imaging of autophagy, apoptosis, senescence and clusters ; 4.matrix: imaging of biochemical composition and elasticity. The goal of this study is to achieve non-invasive in vivo imaging of the biomechanical response of cellular and molecular in intervertebral disc under abnormal mechanical load. We are trying to offer an effective technical approaches for early diagnosis and early prediction of IVDD, and creating a new field of imaging studies.

IVDD 是成年人中发病率最高的疾病，临床缺乏有效治疗，原因是未从源头上阻断或延缓其发生发展。研究证明IVDD的生物力学发病机制至关重要，但目前相关的基础研究缺乏向临床应用转化的手段，无法服务于早诊早治。因此，将细胞及分子生物力学与影像技术有机结合，实现椎间盘生物力学应答过程的无创性活体成像，是临床有效治疗IVDD的前提，是亟待解决的科学问题。. 该申请项目致力于通过椎间盘细胞和分子生物力学应答机制成像，包括:1.营养：营养源（孔隙率和微血管）成像、营养-代谢物运输（葡萄糖、氧气、乳酸）成像；2.细胞自身力-化学转换：整合素和Ca2+成像；3.细胞生物学反应：自噬、凋亡、衰老、集簇成像；4.基质：生化及弹性成像，明确异常应力诱导的椎间盘生物力学反应特征，从理论上建立一套IVDD生物力学发病机制的影像学方法。开创活体无创性成像椎间盘生物力学应答机制的新的研究领域。

IVDD是成年人中发病率最高的疾病，临床缺乏有效治疗，原因是未从源头上阻断或延缓其发生发展。研究证明IVDD的生物力学发病机制至关重要，但目前相关的基础研究缺乏向临床应用转化的手段，无法服务于早诊早治。因此，将细胞及分子生物力学与影像技术有机结合，实现椎间盘生物力学应答过程的无创性活体成像，是临床有效治疗IVDD的前提，是亟待解决的科学问题。.该申请项目致力于通过椎间盘细胞和分子生物力学应答机制成像，包括:1.营养：营养源（孔隙率和微血管）成像、营养-代谢物运输（葡萄糖、氧气、乳酸）成像；2.细胞自身力-化学转换：整合素和Ca2+成像；3.细胞生物学反应：自噬、凋亡、衰老、集簇成像；4.基质：生化及弹性成像，明确异常应力诱导的椎间盘生物力学反应特征，从理论上建立一套IVDD生物力学发病机制的影像学方法。开创活体无创性成像椎间盘生物力学应答机制的新的研究领域。

内容获取失败，请点击重试