Molecular imprint polymer for urine biomarker isolation

用于尿液生物标志物分离的分子印迹聚合物

• 批准号: 484791-2015
• 负责人: Chen, David
• 金额: $ 1.82万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=584535
• 关键词: Molecular; imprint; polymer; urine; biomarker

Early detection of cancer saves lives. This project is related to a patients screening technology for cancers at very early stages using the quantification of an exogenous metabolite in urine. BioMark Technologies Inc. developed this technology based on the fact that increased the increased acetylation of an exogenous compound amantadine to acetylamantadine (AcAm) is strongly correlated to the up-regulation of the enzyme spermine/spermidine N-acetyltransferase (SSAT1), which is correlated to several types of cancers. The currently available analytical methods for the quantification of AcAm are laborious and costly, and efforts to reduce the cost with the direct use of Raman spectrometry have proven difficult because of the contamination of matrix material on the probes. In this project we propose to use molecular imprint polymer (MIP) to clean up the sample and enrich the target analyte AcAm, so that both the time and the cost required for the quantitative analysis can be significantly reduced to make this technology readily available in all clinical labs. The developed technology can be readily transferred to BioMark Technologies Inc., and will be of direct benefit to the Canadian economy and the health and well-being of Canadians.

早期发现癌症可以挽救生命。该项目与癌症患者筛查技术有关 在非常早期阶段使用尿液中外源代谢物的定量。生物标记技术公司 基于增加外源性乙酰化的事实开发了这项技术 复合金刚烷胺至乙酰金刚烷胺 (AcAm) 与酶的上调密切相关 精胺/亚精胺 N-乙酰转移酶 (SSAT1)，与多种癌症相关。这 目前可用的 AcAm 定量分析方法费力且成本高昂，并且需要努力 由于污染，直接使用拉曼光谱法降低成本已被证明是困难的 探针上的基质材料。在这个项目中，我们建议使用分子印迹聚合物（MIP）来清洁 上样并富集目标分析物 AcAm，因此所需的时间和成本 可以显着减少定量分析，使该技术可以在所有临床实验室中使用。 所开发的技术可以很容易地转让给 BioMark Technologies Inc.，并将直接用于 有利于加拿大经济以及加拿大人的健康和福祉。