基于还原氧化石墨烯和金属纳米粒层层自组装的分子印记电化学传感体系及临床应用

Reduced graphene oxide and metal nano-materials have been received more and more attentions in the field of sensors because of their good electrical conductivity, catalytic properties and high specific surface area. Due to the efficient molecular recognition capacity of molecularly imprinted polymers (MIPs), the correlative researches about the combination with molecular imprinting technology (MIT) and sensors in separation and sensors fields have been become more noticeable. Nowadays, based on the preliminary works about the functional modification of nanomaterials, the preparation of self-assembly MIPs and the electrochemical sensors, we will construct the nanostructure electrochemical sensing membranes by using the self-assembly technology according to the molecular structures of monoamine neurotransmitters, the multiple design of reduced graphene oxide, metal nano-materials and MIPs, and then prepare a novel electrochemical sensor with high sensitivity and good recognition. Subsequently, we will establish a sensitive, accurate and rapid detection system for trace neurotransmitters in clinical samples by using the resulting electrochemical sensor. At the same time, we will reveal the 'structure-activity' relationship among the self-assembly system, electrical conductivity and electrocatalytic activity by exploring the composition, structure and methods of characterizing imprinting sensing membranes at the molecular levels, and finding out the nature and rules of self-assembly process. This research project has theoretical significance and practical value, not only in providing a new research and methods for the use of MIT in ECS, but also in aspects of studying the physiological function of neurotransmitters, disease pathogenesis, drug mechanism, disease diagnosis, etc.

还原氧化石墨烯、金属纳米材料复合物集优良导电、催化和高比表面积等特性于一体，引起传感研究领域高度关注；分子印记聚合物的特异识别性使其在分离和传感等领域的研究令人瞩目。本课题将在前期纳米材料与电极功能化修饰、自组装分子印记聚合物与电化学传感器研究基础上，根据单胺类神经递质的分子结构，经还原氧化石墨烯、金属纳米材料与分子印记聚合物多重设计，构筑可控自组装新纳米结构的电化学传感膜，制备具有高灵敏和高识别特性的电化学传感器，建立对临床样本中微痕量神经递质灵敏、准确和快速检测体系。同时在分子水平探索电化学印记传感膜的组成、结构和表征方法，认识自组装过程本质与规律，揭示自组装体结构与电传导与电催化活性的"构～效"关系。本项目不仅为分子印记技术用于电化学传感器研究提供新思路和新方法，而且对研究神经递质的生理功能、疾病发病机理、药物作用机理、疾病的诊断等都有重要理论意义和实用价值。

还原氧化石墨烯、金属纳米材料复合物集优良导电、催化和高比表面积等特性于一体，引起传感研究领域高度关注；分子印记聚合物的特异识别性使其在分离和传感等领域的研究令人瞩目。本课题在前期纳米材料与电极功能化修饰、自组装分子印记聚合物与电化学传感器研究基础上，根据单胺类神经递质的分子结构，经还原氧化石墨烯、金属纳米材料与分子印记聚合物多重设计，构筑可控自组装新纳米结构的电化学传感膜，制备出具有高灵敏和高识别特性的电化学传感器，建立了对临床样本中微痕量神经递质灵敏、准确和快速检测体系, 并成功用于多种基质样品中目标物的测定。探索研究了多种纳米材料自组装电化学印记传感膜的的方法和规律, 并对其组成、结构进行表征，揭示出传感膜纳米结构与电传导与电催化活性的"构～效"关系。本项目不仅为分子印记技术用于电化学传感器研究提供新思路和新方法，而且对神经递质等临床标志物的生理功能、疾病发病机理、药物作用机理、疾病的诊断等研究都有重要理论意义和实用价值。. 在扩展性研究中，将石墨烯与金属纳米复合物灵活用于多种不同结构待测目标分子印记电化学传感膜研究，表明本项研究技术不仅可用于临床研究，而且扩展用于食品，药品和化妆品安全等领域，具有明显普适性和良好应用前景。另外对基于F3O4@rGO掺杂的磁场导向自组装分子印记传感膜进行了探索研究，为磁场诱导制备可控电化学传感膜奠定了重要基础。

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