Project 3 - Lung Health and Inflammation

项目 3 - 肺部健康与炎症

基本信息

批准号：
10628253
负责人：
GREGORY A HAWKINS
金额：
$ 17.37万
依托单位：
WAKE FOREST UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-05-03 至 2028-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10628253
关键词：
Address Adolescence Adolescent Adult Affect Airway Disease Aryl Hydrocarbon Receptor Asthma Atherosclerosis Biological Biological Markers Blood Brain Chronic Chronic Obstructive Pulmonary Disease Cigarette Smoker Cognitive Collaborations Collection Cotinine Coughing DNA Methylation Dependence Development Disease Dose Early Diagnosis Epigenetic Process Epithelial Cells Future Gene Expression Genes Genetic Transcription Health High School Student Immune Impairment Inflammation Inflammatory Knowledge Life Link Lung diseases Malignant Neoplasms Malignant neoplasm of lung Measures Mediation Methods Molecular Molecular Profiling Mucins Nasal Epithelium Outcome Oxidative Stress Pathogenesis Pathogenicity Pathway Analysis Pathway interactions Patient Self-Report Phenotype Physiological Production Public Health Pulmonary Inflammation Questionnaires Receptor Signaling Research Risk Risk Assessment Saliva Smoker Smoking Spirometry Sputum Stress Structure Surveys Symptoms Testing Time Tobacco Variant Vascular Diseases Visit Xenobiotic Metabolism airway inflammation biological adaptation to stress bronchial epithelium cigarette smoking cohort epigenome exposure to cigarette smoke health communication innovation lung development lung health minimally invasive multidisciplinary multiple omics oxidative damage peripheral blood psychosocial pulmonary function respiratory respiratory health sample collection smoking-related disease systemic inflammatory response transcriptome transcriptomics vaper vaping

项目摘要

ABSTRACT – PROJECT 3: Lung Health and Inflammation Research has shown that adolescent cigarette smoking alters lung development, adversely affects respiratory health, and contributes to chronic low-grade, systemic inflammation linked with the development of vascular disease and cancer. However, the effects of vaping have not been fully evaluated. Rather than waiting decades to fully assess the risk of vaping on the development of disease, we propose to characterize early respiratory, physiological, and molecular effects of vaping during adolescence. Given the increasing number of adolescents who vape frequently (20+ days a month), a better understanding of the health consequences of vaping is critical to inform public health communication efforts. To address the gaps in knowledge of the potential harms of vaping, the Multidisciplinary Assessment of Risks of Vaping in Early Life (MARVEL) Project 3 will test the hypothesis that vaping impairs respiratory health as early as adolescence, and that vaping results in overlapping molecular signatures with cigarette smoke exposure and smoking-related disease pathogenesis. Specifically, we will: 1) Characterize the effects of vaping on respiratory health in adolescence; 2) Identify molecular alterations in airways associated with vaping and respiratory health outcomes; and 3) Characterize oxidative stress and systemic inflammatory alterations associated with vaping. To detect early respiratory, physiological, and molecular effects of vaping during adolescence, we will leverage comprehensive phenotypic characterization including bronchitic symptoms of over 4000 high school students from the Online Survey, and repeated measures of lung function (St. George’s Respiratory Questionnaire and spirometry) and collection of biospecimens (nasal epithelial cells and blood) from 360 high school students, at two lab visits 18 months apart. Nasal epithelial cells are robust surrogates for lower airway bronchial epithelial cells, and will be used to examine the effects of vaping on the epigenome and transcriptome. Peripheral blood will be used to examine the effects of vaping on systemic inflammation. Dose-dependency will be assessed by detailed self- report, biomarkers (saliva cotinine, TNE, CEMA) and product characterization. Knowledge gained from Project 3 will inform the development of health communications in Project 4 relevant to the early respiratory and systemic inflammatory effects of vaping during adolescence.

摘要 – 项目 3：肺部健康与炎症研究表明，青少年吸烟会改变肺部发育，对呼吸系统产生不利影响健康，并导致与血管发育相关的慢性低度全身炎症然而，电子烟的影响尚未得到充分评估，而不是等待。为了充分评估电子烟对疾病发展的风险，我们建议尽早表征鉴于青少年时期电子烟对呼吸、生理和分子的影响。经常吸电子烟（每月 20 天以上）的青少年，更好地了解电子烟对健康的影响电子烟对于公共卫生传播工作至关重要，以弥补知识差距。电子烟的潜在危害，早期电子烟风险的多学科评估 (MARVEL) 项目 3 将检验以下假设：电子烟早在青春期就会损害呼吸系统健康，并且电子烟导致分子特征与香烟烟雾暴露和吸烟相关疾病重叠具体来说，我们将： 1）描述电子烟对青春期呼吸健康的影响； 2) 识别与电子烟和呼吸系统健康结果相关的气道分子变化；3) 表征与电子烟相关的氧化应激和全身炎症改变。青春期电子烟对呼吸、生理和分子的影响，我们将利用全面的来自在线的 4000 多名高中生的表型特征，包括支气管症状肺功能的调查和重复测量（圣乔治呼吸问卷和肺活量测定）和在两次实验室访问中收集 360 名高中生的生物样本（鼻上皮细胞和血液）18 鼻上皮细胞是下气道支气管上皮细胞的有力替代物，并且将是相隔数月的。用于检查电子烟对表观基因组和转录组的影响。检查电子烟对全身炎症的影响将通过详细的自我评估进行评估。报告、生物标志物（唾液可替宁、TNE、CEMA）和从项目中获得的产品特性。 3 将为项目 4 中与早期呼吸系统疾病相关的健康传播的发展提供信息青春期电子烟的全身炎症作用。