Perkin Elmer Sciclone G3 NGSx Workstation for High Throughput Sequencing Library Preparation

Perkin Elmer Sciclone G3 NGSx 工作站用于高通量测序文库制备

• 批准号: 9272640
• 负责人: GREGORY A HAWKINS
• 金额: $ 23.44万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-10 至 2018-04-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9272640
• 关键词: Baptist Church; Comprehensive Cancer Center; DNA sequencing; Department chair; Faculty; Financial Support; Funding; Grant; Health Sciences; High-Throughput Nucleotide Sequencing; Laboratories; Libraries; Minor; Preparation; Protocols documentation; Resource Sharing; Running; United States National Institutes of Health; Universities; Vendor; cancer genomics; design; exome sequencing; flexibility; forest; instrument; instrumentation; next generation; programs; sequencing platform; transcriptome sequencing; user-friendly

Abstract The Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) Cancer Genomics Core Laboratory, an NCI-supported Shared Resource is requesting funds to purchase a Perkin Elmer Sciclone G3 NGSx Workstation from Perkin Elmer. This workstation is an automated platform designed to prepare libraries for Next Generation DNA Sequencing (NGS) platforms. The instrument is flexible in design and has the capability to prepare 8-96 libraries per run. This Sciclone G3 NGSx Workstation prepares libraries for all NGS applications including RNAseq, ChIPseq, DNAseq, exome sequencing, and small RNAseq, and comes with preset programs for NGS libraries preparation from multiple vendors. The workstation has a user friendly programmable power controller that can be programmed to implement new NGS library protocols as they are developed. The instrument will be located in the Cancer Genomics Core Laboratory, which is supported by the WFBCCC Shared Resource Grant. The workstation will support 10 Major Users with NIH funding and 6 Minor Users with NIH grants across multiple departments at Wake Forest University Health Sciences (WFUHS). This instrumentation grant has outstanding support from the WFUHS faculty and outstanding financial support from the WFUHS and WFBCCC department chairs and administration

抽象的 维克森林浸信会综合癌症中心 (WFBCCC) 癌症基因组学核心实验室 NCI 支持的共享资源正在请求资金购买 Perkin Elmer Sciclone G3 NGSx Perkin Elmer 工作站。该工作站是一个自动化平台，旨在为以下内容准备图书馆： 下一代 DNA 测序 (NGS) 平台。该仪器设计灵活，具有 每次运行准备 8-96 个库。该 Sciclone G3 NGSx 工作站为所有 NGS 准备文库 应用程序包括 RNAseq、ChIPseq、DNAseq、外显子组测序和小 RNAseq，并附带 来自多个供应商的用于 NGS 文库制备的预设程序。该工作站具有用户友好的 可编程电源控制器，可通过编程实现新的 NGS 库协议 发达。该仪器将位于癌症基因组学核心实验室，该实验室由 WFBCCC 共享资源补助金。该工作站将支持 10 个由 NIH 资助的主要用户和 6 个次要用户 在维克森林大学健康科学 (WFUHS) 多个部门获得 NIH 资助的用户。这 仪器补助金得到了 WFUHS 教师的杰出支持以及来自 WFUHS 的杰出财政支持 WFUHS 和 WFBCCC 系主任和行政人员