Core 2 Dissemination pp.367-405

核心2传播第367-405页

• 批准号: 8999634
• 负责人: THOMAS C IRVING
• 金额: $ 5.27万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8999634
• 关键词: Address; Alzheimer' s Disease; Arthritis; Automobile Driving; Biocompatible Materials; Biomedical Research; Biophysics; Biotechnology; Communities; Consumption; Crystallography; Data; Degenerative Disorder; Development; Disease; Educational workshop; Ensure; Fiber; Heart Diseases; Heterogeneity; Image; International; Laboratories; Link; Malignant Neoplasms; Modeling; Muscle; Muscle function; Peer Review; Phase-Contrast Microscopy; Photons; Publications; Recruitment Activity; Refractive Indices; Research; Resolution; Resources; Retinitis Pigmentosa; Review Literature; Roentgen Rays; Safety; Sampling; Scanning; Services; Source; Speed; Sushi Domain; System; Technology; Time; Training Activity; X ray diffraction analysis; X-Ray Diffraction; beamline; data acquisition; data management; design; detector; instrument; lens; light scattering; meetings; multi-scale modeling; nephelometry; novel; operation; programs; protein misfolding; research study; simulation; single molecule; tool; web site

The BioCAT Biotechnology Research Resource operates X-ray beamline 18ID at the Advanced Photon Source, Argonne National Laboratory. Now in its 20th year of operation, it is a mature, productive facility with many capabilities unique in the USA, and, arguably, the world. Going forward, we intend to maintain our world- class capabilities in static, time- and spatially-resolved fiber diffraction with beamline enhancements for increased flux and beam quality. A novel high speed, high sensitivity, high spatial resolution pixel array detector will provide an excellent match to the needs of our muscle diffraction program. Also proposed is a versatile micro-diffraction/micro-SAXS instrument that can use one of two Compound Refractive Lenses optimized for either wide- or small-angle fiber crystallography, and continuous flow SAXS experiments. We will implement multimodal scanning micro-diffraction, x-ray florescence microscopy, phase contrast and uv/visible imaging that can be done either singly or in combination with the same instrument on the same samples. Developments in time-resolved SAXS will extend available time regimes from 500 ns to seconds with major reductions in sample consumption, by more than order of magnitude, from current capabilities. This will allow a much wider range of biomedical problems to be addressed than previously possible. A new beamline data acquisition and control system will provide a common interface and better data management for all experiments and advanced support for time resolved experiments. Combined refractive index, dynamic light scattering and multi-angle light scattering measurements with SAXS will offer more comprehensive sample characterization on-line for more robust results. A multi-scale modeling effort will allow extracting more information from muscle X-ray diffraction studies. The proposed developments in multi-scale simulations for interpreting single molecule SAXS data will profoundly benefit studies of multicomponent systems that show considerable conformational heterogeneity. Our relationship with the CCP-SAS project will enable us to widely disseminate the advanced modeling tools we create to not only all our DBP's, collaborative and service users but to the wider biomedical community. Our Driving Biomedical Projects, collaborative, and service projects have relevance to basic mechanisms of muscle function, heart disease, retinitis pigmentosa, cancer and neuro-degenerative diseases. Our proposed training activities are designed to ensure safe, efficient and productive use of the resource by our users. Our proposed dissemination activities are designed to grow our user community by keeping targeted biomedical communities well-informed of resource capabilities.

Biocat Biotechnology研究资源在高级光子上运行X射线光束线18ID 资料来源，Argonne国家实验室。现在在运营的第20年，它是一个成熟，富有成效的设施 在美国，可以说是世界的许多功能。展望未来，我们打算维护我们的世界 - 静态，时间和空间分辨的纤维衍射均具有横梁线增强功能 增加通量和光束质量。一种新型的高速，高灵敏度，高空间分辨率像素阵列 检测器将为我们的肌肉衍射计划的需求提供极好的匹配。还提出了一个 多功能微伸缩/微及量仪器可以使用两个复合折射镜头之一 针对广角或小角度纤维晶体学和连续的流量SAXS实验进行了优化。我们将 实施多模式扫描微伸缩，X射线荧光显微镜，相对比和紫外线/可见 可以单独或与同一样品上同一仪器组合进行的成像。 时间分辨的萨克斯公司的发展将从500 ns扩展到秒 从当前功能中减少样本消耗的数量级不仅仅是数量级。这将允许 比以前可能的要解决的生物医学问题要多得多。新的光束线数据 采集和控制系统将为所有人提供一个共同的界面和更好的数据管理 实验和对时间解决实验的高级支持。合并折射率，动态光 SAXS的散射和多角度光散射测量将提供更全面的样本 在线表征以获得更强大的结果。多尺度的建模工作将允许提取更多 来自肌肉X射线衍射研究的信息。多尺度模拟中的拟议发展 解释单分子saxs数据将深刻地有益于显示的多组分系统的研究 相当大的构象异质性。我们与CCP-SAS项目的关系将使我们能够广泛 传播我们创建的高级建模工具，不仅是所有DBP，协作和服务用户 但是对更广泛的生物医学界。我们的驾驶生物医学项目，协作和服务项目 与肌肉功能，心脏病，色素性视网膜炎，癌症和 神经变性疾病。我们提出的培训活动旨在确保安全，高效和 用户对资源的生产效率。我们提出的传播活动旨在发展我们的 用户社区通过保持针对性的生物医学社区的资源能力良好。