The Biophysics Collaborative Access Team

生物物理学协作访问团队

基本信息

批准号：
10561672
负责人：
THOMAS C IRVING
金额：
$ 121.67万
依托单位：
ILLINOIS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-02-01 至 2025-12-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10561672
关键词：
Address Advisory Committees Aging Amyloid Automation Biochemical Biocompatible Materials Biological Biology Biophysics Biotechnology Cardiomyopathies Cell physiology Cells Charge Collaborations Communities Complex Computer software Connective Tissue Data Data Analyses Dedications Development Disease model Drug Design Educational workshop Ensure Equilibrium Equipment Evolution Fiber Funding Grant Human Pathology Illinois Image Individual Infrastructure Instruction Laboratories Leadership Ligands Light Maintenance Mission Modification Molecular Molecular Biology Molecular Sieve Chromatography Muscle Myocardium National Center for Research Resources National Institute of General Medical Sciences Neoplasm Metastasis Noise Nucleic Acid Folding Nucleic Acids Pathology Persons Photons Physics Physiological Policies Principal Investigator Productivity Proteins Publications Radiation induced damage Recruitment Activity Research Research Personnel Research Project Grants Research Technics Resources Sampling Scanning Schedule Science Shapes Signal Transduction Site Skeletal Muscle Source Structure Students Synchrotrons System Technology Time Tissue Sample Training Training Activity Traumatic Brain Injury United States National Institutes of Health Update Visit X ray diffraction analysis base beamline biological systems biophysical properties community organizations computerized data processing data acquisition data quality experience experimental study fibrous protein improved inherited cardiomyopathy instrumentation interest macromolecule operation outreach prevent professor programs protein structure repaired structural biology symposium synchrotron radiation tool training opportunity user-friendly web site

项目摘要

The mission of the Biophysics Collaborative Access Team (BioCAT) is to provide user access to a state-of-the- art synchrotron beamline (18ID) for diffraction and scattering of non-crystalline biological materials at the Advanced Photon Source, Argonne National Lab. Continued evolution of the beamline since first light in 1997 has resulted in a unique in the USA fiber diffraction program, and a world-class Bio-SAXS program with unique capabilities both in the equilibrium and time-resolved realms. The use of size exclusion chromatography in line with the SAXS camera has ensured unprecedentedly high data quality even with biochemically challenging samples otherwise thought to be intractable for SAXS. The recent incorporation of MALS and DLS in line with SEC allows additional biophysical characterization that can be invaluable in interpreting SAXS data from difficult systems. Our new coflow sheath flow sample cell has revolutionized SAXS data acquisition by preventing radiation damage, enhancing efficiency accompanied by significant increases in signal to noise. BioCAT maintains specialized apparatuses for combined time-resolved X-ray diffraction and physiological studies of cardiac and skeletal muscle. These studies have extended in scope from basic biophysical problems to address new disease models including cardiomyopathies. A traditional strength is fiber diffraction of general fibrous protein systems that has recently expanded into scanning diffraction imaging of fibrous protein structures in complex tissue samples. A major strength of the resource is the experienced and dedicated scientific staff who assist users seven days a week in data acquisition, processing, analysis and interpretation. BioCAT is directed by Dr. Thomas Irving, who has more than 20 years of experience overseeing synchrotron facilities for non-crystalline diffraction and scattering. Dr. Jesse Hopkins oversaw installation of the new coflow SAXS sample cell, established new user-friendly GUI based data acquisition interfaces, and provide advanced tools to interpret SEC-SAXS data. Dr. Hopkins develops and maintains the BioXTAS RAW SAXS data processing and analysis software package and will extend it to allow increased automation to optimize productivity while minimizing on-site effort for users. Dr. Srinivas Chakravarthy BioCAT’s resident biochemist coordinates the SAXS user program, assists users with conducting experiments on site, and also maintains the biochemical infrastructure. Dr. Weikang Ma is an expert in fiber diffraction of muscle and is responsible for providing comprehensive support to all our fiber diffraction users. All four of these individuals are involved in training and dissemination activities, including scientific publications, presentations at conferences, organization of BioCAT workshops and participating in community organized workshops. BioCAT has an updated website with a comprehensive set of instructions for all aspects of the resource that are sufficient for a user to plan and execute successful experiments at the beamline. Together the SAXS and Fiber Diffraction user programs host 70-90 user groups per year and consistently produce 30-40 publications per year.

生物物理协作访问团队（BioCat）的使命是为用户访问最新的访问权限 ART同步光束线（18ID）用于非晶生物学材料的衍射和散射 Argonne National Lab的高级光子来源。自1997年第一光以来，梁线的持续演变已经在美国纤维衍射计划中产生了独特的独特平衡和时间分辨领域的能力。尺寸排除色谱的使用排队借助SAXS摄像机，即使在生化上挑战也确保了前所未有的高数据质量否则，人们认为对萨克斯群岛很棘手。最近与MALS和DLS合并 SEC允许其他生物物理表征，这对于解释萨克斯数据的数据是无价的困难的系统。我们的新Coflow护套流量样品单元已通过防止辐射损伤，从而提高信号对噪声的显着增加而达到的效率。 BioCat维护专门的设备，用于合并的时间分辨X射线衍射和生理心脏和骨骼肌的研究。这些研究从基本的生物物理问题范围扩大了解决包括心肌病在内的新疾病模型。传统力量是一般的纤维衍射最近已扩展为纤维蛋白扫描衍射成像的纤维蛋白系统复杂组织样品中的结构。资源的主要优势是经验和敬业科学人员每周七天为用户提供数据获取，处理，分析和解释。 BioCat由托马斯·欧文（Thomas Irving）博士指导，他拥有超过20年的监督同步器的经验非结晶衍射和散射的设施。杰西·霍普金斯（Jesse Hopkins）博士负责新的科夫洛的安装 SAXS样本单元格建立了新的基于用户友好的GUI数据采集接口，并提供高级解释SEC-SAXS数据的工具。霍普金斯博士的发展并维护生物XTA的RAW SAXS数据处理和分析软件包，并将扩展其以允许增加自动化以优化生产力，同时最大程度地减少用户的现场努力。 Srinivas Chakravarthy Biocat的居民生物化学家协调SAXS用户程序，协助用户在现场进行实验，并维护生化基础设施。 Weikang MA博士是肌肉纤维衍射的专家，负责为我们所有的纤维衍射用户提供全面的支持。这四个人都参与培训和传播活动，包括科学出版物，会议上的演讲，组织研讨会组织并参加社区有组织的研讨会。 BioCat有一个更新网站，并提供一套全面的说明，适用于资源的各个方面用户可以在梁线上计划并执行成功的实验。萨克斯和纤维衍射在一起用户程序每年举办70-90个用户组，每年持续生产30-40个出版物。