Hybrid Rat Diversity Program - cryopreservation resource for rat models

杂交大鼠多样性计划 - 大鼠模型的冷冻保存资源

基本信息

批准号：
10609632
负责人：
MELINDA R DWINELL
金额：
$ 20.34万
依托单位：
MEDICAL COLLEGE OF WISCONSIN
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-01 至 2026-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10609632
关键词：
Age Award Biomedical Research Birth Rate COVID-19 pandemic Cattle Characteristics Collection Communication Communities Complex Cryopreservation Custom Czech Republic Data Data Analyses Development Disasters Disease Disease model Documentation Domestic Animals Dose Embryo Embryo Transfer Ensure Evaluation Evaluation Indexes Female Ficoll Follicle Stimulating Hormone Foundations Genetic Genetic Variation Genomics Genotype Goals Gonadotropins Grant Hormones Human Chorionic Gonadotropin Hybrids Implant In Vitro Inbred Strain Inbred Strains Rats Inbreeding Infrastructure Institution Japan Laboratories Laboratory Research Methods Modeling Monoclonal Antibody R24 Mus PMSG (Gonadotropins)Parents Phenotype Positioning Attribute Process Propylene Glycols Protocols documentation Publications Rat Genome Database Rat Strains Rattus Recombinant Inbred Strain Recombinants Recovery Reporting Research Research Personnel Resources Serum Sucrose Superovulation Survival Rate System Toxic effect Translations animal resource clinical translation clinically relevant embryo cryopreservation embryo quality ethylene glycol forward genetics genomic data genomic locus improved in vivo inhibin natural Blastocyst Implantation novel strategies personalized approach pregnant preservation programs pup repository reproductive restoration success tool trait transcriptomics zygote

项目摘要

Project Summary The ongoing goal of this program is to extend the use of rat models for clinical translation through establishing an animal resource and genomic dataset to facilitate the translation of laboratory research to clinical relevance. Specifically, the Hybrid Rat Diversity Panel (HRDP) resource provides the research community with a foundation for systems genetics and a tool for forward genetics. The HRDP parent award aims to 1) to continue to establish the 96 strain Hybrid Rat Diversity Panel comprised of 33 classic inbred strains, 30 HXB/BXH recombinant inbred strains and 33 FXLE/LEXF recombinant inbred strains through embryo rederivation and importation, 2) to characterize, maintain and distribute the HRDP, 3) to provide genomic and transcriptomic sequencing of the HRDP and 4) and provide data analysis, integration and dissemination primarily through the Rat Genome Database (RGD). Specifically, this supplement will 1) evaluate embryo quality pre- and post-cryopreservation, 2) improve the efficiency of embryo collection for cryopreservation, and 3) develop cryopreservation protocols customized to strain characteristics. Together these results will have broad utility the scientific community through the genetic and phenotypic diversity represented in the strains selected for the supplement project in combination with the available data from the HRDP parent award. Data and protocols will be made available to the research community through the Rat Genome Database, publications, and through rat repositories.

项目概要该计划的持续目标是扩大大鼠模型在临床转化中的使用通过建立动物资源和基因组数据集来促进翻译实验室研究与临床相关性。具体来说，杂交大鼠多样性小组 (HRDP) 该资源为研究界提供了系统遗传学的基础和工具正向遗传学。 HRDP家长奖旨在1）继续建立96品系杂交大鼠多样性面板由 33 个经典近交系、30 个 HXB/BXH 重组体组成通过胚胎再衍生和 33 个 FXLE/LEXF 重组近交系导入，2) 表征、维护和分发 HRDP，3) 提供基因组和 HRDP 的转录组测序和 4) 并提供数据分析、整合和主要通过大鼠基因组数据库（RGD）传播。具体而言，本补充将 1) 评估冷冻保存前后的胚胎质量，2) 提高冷冻保存的效率用于冷冻保存的胚胎收集，以及 3) 开发定制的冷冻保存方案应变特性。这些结果将对科学界产生广泛的效用通过所选菌株所代表的遗传和表型多样性补充项目结合 HRDP 母奖的可用数据。数据实验方案将通过大鼠基因组提供给研究界数据库、出版物和大鼠存储库。