Hybrid Rat Diversity Program

杂交大鼠多样性计划

基本信息

批准号：
10411190
负责人：
MELINDA R DWINELL
金额：
$ 77.94万
依托单位：
MEDICAL COLLEGE OF WISCONSIN
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-01 至 2026-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10411190
关键词：
Age Aging Alcohols Animal Model Animals Anxiety Arthritis Asthma Atlases Biological Breeding Colorado Communities Complex Controlled Environment Cost of Illness Cryopreservation Czech Republic DNA Sequence Data Data Analyses Development Diabetes Mellitus Discipline Disease Disease Progression Disease susceptibility Embryo Ensure Environment Environmental Impact Environmental Risk Factor Exercise Exposure to Female Foundations Funding Future Gene Targeting Genetic Genetic Heterogeneity Genetic Polymorphism Genetic Predisposition to Disease Genetic Transcription Genetic Variation Genetic study Genome Genomics Goals Grant Human Hybrids Hypertension Inbred Strain Inbred Strains Rats Inbreeding Infrastructure Japan Kidney Knowledge Laboratory Research Location Malignant Neoplasms Maps Missouri Modeling Molecular Monoclonal Antibody R24 Muscle Pharmaceutical Preparations Pharmacogenomics Phenotype Physiological Population Heterogeneity Positioning Attribute Proteome Publications Rat Genome Database Rattus Recombinant Inbred Strain Recombinants Recovery Reproducibility Research Research Personnel Resources Resuscitation Sample Size Sex Differences Site-Directed Mutagenesis Source Stable Populations Stroke System Therapeutic Thymus Gland Tissues Translating Translations Universities Update Variant Visualization software Wisconsin addiction animal resource biobank catalyst clinical translation clinically relevant cost data infrastructure data reuse disease mechanisms study disorder risk drug development embryo cryopreservation epigenome forward genetics genome browser genome sequencing genomic data genomic locus interest male medical schools metabolome molecular phenotype outreach phenotypic data potential biomarker precision medicine preference programs public repository reproductive system disorder stressor symposium therapeutic target tool trait transcriptome transcriptomics webinar whole genome

项目摘要

Project Summary The ongoing goal of this program is to extend the use of rat models for clinical translation through establishing an animal resource and genomic dataset to facilitate the translation of laboratory research to clinical relevance. Specifically, the Hybrid Rat Diversity Panel (HRDP) resource provides the research community with a foundation for systems genetics and a tool for forward genetics. To achieve this goal, we propose the following four specific aims: 1) to continue to establish the 96 strain Hybrid Rat Diversity Panel comprised of 33 classic inbred strains, 30 HXB/BXH recombinant inbred strains and 33 FXLE/LEXF recombinant inbred strains through embryo rederivation and importation, 2) to characterize, maintain and distribute the HRDP, 3) to provide genomic and transcriptomic sequencing of the HRDP and 4) and provide data analysis, integration and dissemination primarily through the Rat Genome Database (RGD). This resource will be used to study mechanisms of complex disease traits through systems genetics by providing investigators with access to a renewable panel of inbred rat strains and biobanked tissues, reusable phenotypic data, reusable genomic and transcriptomic data, and the infrastructure to explore, analyze and visualize these resources through the HRDP Portal at RGD. Experimental rats and biobanked tissues from the entire HRDP panel can be used for other omic studies, including the epigenome, proteome, and metabolome. The Rat Resource & Research Center (RRRC) is partnering with the HRDP to provide the rat models to the research community. The HRDP will have broad utility to the scientific community through the genetic and phenotypic diversity represented in the 96 strain panel used to study hypertension, stroke, diabetes, cancer, arthritis, reproductive disorders, asthma, aging, alcohol preference, anxiety, exercise and addiction.

项目概要该计划的持续目标是扩大大鼠模型在临床转化中的使用通过建立动物资源和基因组数据集来促进翻译实验室研究与临床相关性。具体来说，杂交大鼠多样性小组 (HRDP) 该资源为研究界提供了系统遗传学的基础和工具正向遗传学。为实现这一目标，我们提出以下四个具体目标：1）继续建立由33个经典近交系组成的96品系杂交大鼠多样性小组品系，30个HXB/BXH重组自交系和33个FXLE/LEXF重组自交系通过胚胎再衍生和输入，2) 表征、维护和分发 HRDP，3) 提供 HRDP 的基因组和转录组测序，4) 并提供主要通过大鼠基因组数据库进行数据分析、整合和传播（RGD）。该资源将用于通过以下方式研究复杂疾病特征的机制通过为研究人员提供可再生的近交大鼠小组来进行系统遗传学菌株和生物库组织、可重复使用的表型数据、可重复使用的基因组和转录组数据数据，以及通过 HRDP 探索、分析和可视化这些资源的基础设施 RGD 的门户。来自整个 HRDP 组的实验大鼠和生物库组织可以用于其他组学研究，包括表观基因组、蛋白质组和代谢组。老鼠资源与研究中心 (RRRC) 正在与 HRDP 合作，为以下群体提供大鼠模型：研究界。 HRDP 将通过以下方式对科学界产生广泛的效用：用于研究的 96 个菌株组中所代表的遗传和表型多样性高血压、中风、糖尿病、癌症、关节炎、生殖障碍、哮喘、衰老、酒精偏好、焦虑、运动和成瘾。