Biomarkers of Periodontal Disease Progression

牙周病进展的生物标志物

• 批准号: 8914088
• 负责人: RICARDO P TELES
• 金额: $ 200万
• 依托单位: ADA FORSYTH INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-27 至 2016-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8914088
• 关键词: Accounting; Address; Adult; Affect; Award; Bedside Testings; Bioinformatics; Biological; Biological Markers; Cardiovascular Diseases; Clinical; Clinical Research; Clinical Sciences; Coupled; Data; Data Set; Development; Diabetes Mellitus; Diagnostic; Diagnostic tests; Disease; Disease Progression; Early Diagnosis; Early identification; Evaluation; Expenditure; Future; Gingival Crevicular Fluid; Goals; Health; Immune response; Individual; Infection; Inflammatory; Institutes; Intervention; Knowledge; Lead; Liquid substance; Lung diseases; Measures; Methods; Michigan; Modeling; Monitor; Oligonucleotides; Oral; Oral health; Outcome; Pathogenesis; Patients; Periodontal Diseases; Periodontitis; Physiological; Prevalence; Preventive; Process; Property; RNA; Recruitment Activity; Research; Research Personnel; Resource Allocation; Resources; Risk; Risk Factors; Saliva; Salivary; Sampling; Science; Side; Signal Transduction; Site; Source; Taxon; Techniques; Testing; Therapeutic; Time; Tissues; Tooth Loss; Tooth structure; Translational Research; Translations; Treatment outcome; United States; United States National Institutes of Health; Validation; aging population; base; clinical practice; disease diagnosis; experience; high risk; improved; interest; microbial; outcome forecast; point-of-care diagnostics; prevent; prognostic; reproductive; subgingival biofilm; treatment response

DESCRIPTION (provided by applicant): The long term goal of our research is to develop a point-of-care (POC) diagnostic test to help clinicians identify sites and/or subjects that are susceptible to periodontal disease progression. The primary objective of this project is to identify biomarkers of periodontal disease progression. The central hypothesis is that a combination of host analytes and subgingival species will be effective as biomarkers of periodontal disease progression. Periodontal diseases are the most common cause of tooth loss among adults in the United States and recent studies suggested that they increase the risk for systemic conditions such as cardiovascular diseases, diabetes, respiratory diseases and can affect reproductive outcome. Further, periodontal diseases progress through "bursts" of activity followed by periods of quiescence. Early accurate identification of individuals and/or sites that are undergoing active disease progression is critical to signal the need for immediate intervention to minimize tooth loss, allow for proper allocation of resources to treat susceptible individuals, and limit the potential systemic sequelae of these infections. However, there are no practical clinical means of identifying periodontal sites and/or subjects that are progressing. Better biomarkers of periodontal disease activity are urgently needed to improve periodontal disease diagnosis, guide therapy, monitor activity, and evaluate treatment response. Aim 1 will examine the longitudinal variability of levels of host biomarkers in GCF and saliva. Aim 2 will examine the diagnostic utility of GCF and salivary levels of host biomarkers and uncultivated and cultivable taxa in saliva and in subgingival biofilm samples for discriminating progressing and non-progressing periodontal sites and subjects. Aim 3 will determine the effects of periodontal therapy on the levels of salivary and GCF biomarkers and on uncultivated and cultivable taxa in saliva and in subgingival biofilms. Factors that strengthen this proposal include: 1) participation by two NIH Clinical and Translational Science Award centers (The Forsyth Institute and Michigan Center for Oral Health Research); 2) vast experience of both Centers in multiplex quantification of host biomarkers in oral fluids; 3) access to the newly developed RNA-oligonucleotide quantification technique (ROQT) for quantifying uncultivated and cultivable bacterial taxa; and 4) access to state-of-the-art bioinformatics cores to support analysis of large data sets. This project addresses the NIDCR's strategic interest in "early detection and identification of risk factors for periodontal disease" and reaffirms the center's commitment to "facilitate the translation of science into clinical practice". Successful completion of this project will lead to validation of biomarkers that can be used in POC tests to help clinicians identify sites and subjects at risk for disease progression who require immediate intervention.

描述（由申请人提供）：我们研究的长期目标是开发护理点（POC）诊断测试，以帮助临床医生确定容易受到牙周疾病进展的地点和/或受试者。该项目的主要目的是确定牙周疾病进展的生物标志物。中心假设是，宿主分析物和亚生物物种的结合将有效作为牙周疾病进展的生物标志物。 牙周疾病是美国成年人牙齿脱落的最常见原因，最近的研究表明，它们增加了系统疾病的风险，例如心血管疾病，糖尿病，呼吸道疾病，并可能影响生殖结果。此外，牙周疾病通过活动的“爆发”，然后是静止期。对正在进行主动疾病进展的个体和/或部位的早期准确识别对于信号即时需要立即干预以最大程度地减少牙齿脱落，允许正确分配资源来治疗易感人群并限制这些感染的潜在系统性后遗症至关重要。但是，没有实际临床手段来识别正在进行的牙周部位和/或受试者。迫切需要更好的牙周疾病活性生物标志物来改善牙周疾病诊断，指导治疗，监测活动和评估治疗反应。 AIM 1将检查GCF和唾液中宿主生物标志物水平的纵向变化。 AIM 2将检查唾液和subgingival生物膜样品中GCF和唾液水平的诊断效用，以及不培养和可耕种的分类群，以区分进步和非培养的牙周地点和受试者。 AIM 3将确定牙周疗法对唾液和GCF生物标志物水平以及唾液和副生物膜中不可养殖和可耕种的分类的影响。 加强该建议的因素包括：1）两个NIH临床和转化科学奖中心（福赛斯研究所和密歇根州口腔健康研究中心）的参与； 2）两个中心在口服流体中宿主生物标志物的多重量化中的丰富经验； 3）访问新开发的RNA-寡核苷酸定量技术（ROQT），用于量化不培养和可耕种的细菌分类群； 4）访问最先进的生物信息学核心，以支持大型数据集的分析。 该项目涉及NIDCR在“牙周疾病的早期检测和鉴定”中的战略兴趣，并重申该中心“促进将科学转化为临床实践”的承诺。该项目的成功完成将导致对生物标志物的验证，这些生物标志物可用于POC测试，以帮助临床医生确定需要立即干预的疾病进展风险的地点和受试者。