Quantification of Host Markers in GCF Using CBIB

使用 CBIB 对 GCF 中的宿主标记进行量化

• 批准号: 7036069
• 负责人: RICARDO P TELES
• 金额: $ 8.04万
• 依托单位: ADA FORSYTH INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7036069
• 关键词: DNA; biomarker; clinical research; fluid

DESCRIPTION (provided by applicant): Despite advances in our knowledge of the causes and risk factors associated with periodontitis, there are no signs of a decline in its prevalence. Indeed, longer retention of teeth, coupled with an aging population might lead to future increases in the number of subjects affected by periodontal destruction. The severe limitations of the present clinical methods of diagnosis, result in an inability to distinguish subjects and sites at risk for periodontal disease initiation and progression. Thus, patients are often inappropriately treated. Analyses of biochemical markers of periodontal disease in GCF samples may overcome such limitations and provide clinicians with diagnostic and prognostic biomarkers of disease. The goal of the present investigation is to provide clinicians with a discriminatory and reliable analysis to help to diagnose disease status in individual subjects and sites. Specific Aim 1 will optimize and validate the checkerboard immunoblotting (CBIB) technique to quantify multiple components of the GCF, in a large number of samples. The parallel examination of several biomarkers in a large number of samples could result in a test with more significant diagnostic value. Moreover, the ability to investigate several molecules at once would allow inferences on the mechanisms of modulation of the immune response and tissue destruction by these mediators. Once a sensitive assay has been optimized, a cross-sectional pilot study will be performed as a first step in selecting possible biomarkers of disease progression. In Specific Aim 2, GCF samples will be obtained from 30 periodontally healthy and 30 periodontitis subjects in order to assess the diagnostic properties of the different GCF biomarkers in distinguishing health and disease on a subject and site level. Once the best candidate markers have been determined, the use of combinations of markers for improving diagnostic ability will be evaluated. The ultimate goal of this line of research is to conduct longitudinal studies on levels of GCF biomarkers, in order to determine their value as predictors of disease activity, and to better understand their cross-regulation. This goal is in accord with NIDCR's mission to identify markers of disease and elucidate biological events associated with inflammation, with the hope that this knowledge will lead to new therapies in the treatment of periodontal diseases. Moreover, this methodology is at the forefront of new approaches in proteomics, and carries enormous promise for translational applications in the near future.

描述（由申请人提供）：尽管我们了解与牙周炎相关的原因和风险因素的了解，但没有迹象表明其患病率下降。实际上，牙齿的保留率更长，再加上老龄化的人口可能会导致未来受牙周破坏影响的受试者数量的增加。当前诊断临床方法的严重局限性导致无法区分有牙周疾病启动和进展风险的受试者和部位。因此，患者经常接受不适当的治疗。 GCF样品中牙周疾病的生化标志物的分析可能会克服这种局限性，并为临床医生提供诊断和预后的疾病生物标志物。本研究的目的是为临床医生提供歧视性和可靠的分析，以帮助诊断单个受试者和现场的疾病状况。特定的目标1将在大量样品中优化和验证核对板免疫印迹（CBIB）技术，以量化GCF的多个组件。在大量样品中对几种生物标志物的并行检查可能会导致具有更明显的诊断值的测试。此外，立即研究多个分子的能力将允许对这些介体对免疫反应和组织破坏的调节机制进行推断。一旦优化了敏感测定法，将进行横断面试验研究，作为选择疾病进展可能的生物标志物的第一步。在特定的目标2中，将从30个牙周健康和30名牙周炎受试者中获得GCF样品，以评估不同GCF生物标志物在区分受试者和现场水平的健康和疾病方面的诊断特性。一旦确定了最佳的候选标记，将评估使用标记的组合来提高诊断能力。这项研究线的最终目标是对GCF生物标志物水平进行纵向研究，以确定其作为疾病活动的预测指标，并更好地理解其交叉调节。该目标与NIDCR的使命鉴定疾病标志并阐明与炎症相关的生物学事件，希望这种知识能够在治疗牙周疾病治疗方面进行新的疗法。此外，这种方法是蛋白质组学新方法的最前沿，并且在不久的将来对翻译应用具有巨大的希望。