DEVELOPMENT OF A CHECKERBOARD IMMUNOBLOT

棋盘免疫印迹的开发

• 批准号: 7607014
• 负责人: RICARDO P TELES
• 金额: $ 2.23万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7607014
• 关键词: Biological Response Modifiers; Characteristics; Computer Retrieval of Information on Scientific Projects Database; Data; Development; Diagnostic; Disease; Disease Progression; Funding; Gingival Crevicular Fluid; Grant; Immune; Immune response; Immunoblotting; Individual; Inflammatory; Institution; Invasive; Knowledge; Laboratories; Measures; Mediator of activation protein; Nature; Numbers; Periodontal Diseases; Periodontal Infection; Regulation; Research; Research Personnel; Resources; Sampling; Site; Source; Techniques; Technology; Tissues; United States National Institutes of Health; base; cytokine; pathogen

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Several lines of evidence demonstrate that immune mechanisms are responsible for the tissue destruction characteristic of periodontal disease. Gingival crevicular fluid (GCF) could provide a non-invasive diagnostic means to measure the inflammatory mediators released during disease progression. Several immune mediators have been detected in GCF, and their levels have been associated with disease status. We propose to use the checkerboard immunoblotting (CBIB) technique to quantify multiple components of the GCF in a large number of samples. A similar approach has been applied in our laboratory for the characterization of the microbiota associated with periodontal infection. This technology would allow us to characterize GCF components with microbiological data. No such high throughput technique has been available for cytokines or other markers of host status at periodontal sites. Preliminary data indicate that it will be possible to measure significant numbers of cytokines in routine GCF samples. Therefore, it should be possible to quantify the nature of the bacterial challenge and the nature of the host response on a site-by-site basis within the individual. The levels of some inflammatory mediators can influence the synthesis and secretion of others. The ability to investigate several molecules at once would allow inferences on the mechanisms of modulation of immune response and tissue destruction by these mediators. This approach will fill a gap in our knowledge regarding the cross-regulation of inflammatory mediators and the relationship of the levels of these mediators to subgingival periodontal pathogens.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 几条证据表明，免疫机制是牙周疾病的组织破坏特征的原因。 牙龈裂纹液（GCF）可以提供一种非侵入性诊断手段，以测量疾病进展过程中释放的炎症介质。 在GCF中已检测到几个免疫介质，它们的水平与疾病状况有关。 我们建议使用Checker板免疫印迹（CBIB）技术在大量样品中量化GCF的多个组件。 我们的实验室已经采用了类似的方法来表征与牙周感染相关的微生物群。 这项技术将使我们能够用微生物数据来表征GCF组件。 在牙周部位的细胞因子或其他宿主状态的其他标记都没有这样的高通量技术。 初步数据表明，可以在常规GCF样品中测量大量的细胞因子。 因此，应该有可能在个人内部逐步量化细菌挑战的性质和宿主反应的性质。 某些炎症介质的水平会影响他人的综合和分泌。 一次研究多个分子的能力将允许对这些介体对免疫反应和组织破坏的调节机制进行推断。 这种方法将填补我们关于炎症介质的交叉调节以及这些介体水平与副牙周病原体的关系的空白。