Behavioral Assays for Orofacial Pain

口面部疼痛的行为测定

• 批准号: 8088633
• 负责人: CHARLES G. WIDMER
• 金额: $ 19.69万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-05 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8088633
• 关键词: ASIC channel; Acids; Affect; Agonist; Analgesics; Animal Feed; Animals; Anti-Inflammatory Agents; Anti-inflammatory; Arousal; Auditory; Behavior; Behavior assessment; Behavioral; Behavioral Assay; Buprenorphine; Characteristics; Cues; Diclofenac; Dose; Drug Delivery Systems; Environment; Ethology; Evaluation; Face; Facial Pain; Feeding behaviors; Frequencies; Goals; Head; Home environment; Injection of therapeutic agent; Intervention; Ion Channel; Jaw; Learning; Limb structure; Masseter Muscle; Masticatory muscles; Measurement; Measures; Mechanics; Methodology; Methods; Modeling; Mus; Muscle function; Musculoskeletal; Musculoskeletal Pain; Myalgia; Operant Conditioning; Opioid; Orofacial Pain; Pain; Pain Measurement; Pain-Free; Pharmaceutical Preparations; Phase; Reflex action; Reporting; Research Personnel; Rodent; Saline; Secondary to; Sensitivity and Specificity; Sensory; Skin; Source; Tail; Techniques; Temporomandibular Joint; Testing; Therapeutic; Therapeutic Intervention; Tooth structure; Training; Translations; Trigeminal nerve structure; Variant; Vibrissae; Visual; Withdrawal; aversive conditioning; behavior measurement; bone; efficacy testing; feeding; improved; inhibitor/antagonist; instrumentation; mouse model; novel; pain adaptation; pain behavior; response

DESCRIPTION (provided by applicant): Most of the methods used for orofacial pain assessment in rodents have been adapted from limb and tail mechanical and thermal stimulation. However, the results obtained by these methods can be influenced by experimenter interaction, animal attentiveness and potential visual, auditory or whisker sensory cues detected by the rodent when stimulating around the face. Ideally, ethology secondary to pain should be conducted in the home cage environment where there are minimal interventions by the experimenter. Additionally, assessment should be conducted both diurnally and nocturnally to achieve a more accurate representation of pain-related behaviors. The methodology and instrumentation proposed in this set of studies have the potential to identify a novel set of pain behaviors and improves on the deficiencies that have been identified with other techniques. The proposed measures have the potential to identify subtle changes in feeding behavior that are prevalent in orofacial pain conditions such as musculoskeletal pain. This proposal has three specific aims that focus on: (1) identification of behavioral activities associated with the presence or absence of mild to moderate orofacial pain that are reliable and valid; (2) validate the ability of the behavioral assessments to detect analgesic dose-response effects; and (3) using these newly identified behavioral measures, test the efficacy of a non-steroidal anti-inflammatory drug that targets specific ion channels recently reported to be upregulated in the presence of masticatory muscle pain. The overall goal of this proposal is to establish reliable and valid measures of behavioral feeding activities that accurately reflect mild to moderate orofacial pain or pain-free conditions. These behavioral parameters can then be used to evaluate the efficacy of therapeutic interventions that target specific orofacial pain conditions. The automated assessment of orofacial pain behavior in the home cage environment both diurnally and nocturnally would allow a higher throughput of rodent evaluation and quicken the translation of new pain therapeutics for orofacial pain. PUBLIC HEALTH RELEVANCE: Pain that involves the head and face has been difficult to measure in animals. New pain measuring techniques are needed to evaluate facial pain in animals so that new treatments can be developed. This proposal will identify animal feeding behaviors that can be used to measure mild to moderate jaw muscle pain. These behaviors will then be used to test the abilities of drugs to treat jaw muscle pain.

描述（由申请人提供）：啮齿动物中用于口面疼痛评估的大多数方法已从肢体和尾部机械和热刺激中进行了调整。但是，这些方法获得的结果可能会受到实验者的相互作用，动物的专注力以及潜在的视觉，听觉或晶须感官提示，当时啮齿动物在刺激面部周围时检测到。理想情况下，应在实验者最少干预措施的家庭笼环境中进行伦理学。此外，应在昼夜和夜间进行评估，以实现与疼痛相关行为的更准确的代表。这组研究中提出的方法和仪器具有识别一组新型疼痛行为的潜力，并改善了与其他技术相确定的缺陷。所提出的措施有可能识别出在肌肉骨骼疼痛等口腔疼痛条件下普遍存在的喂食行为的细微变化。 该提案具有三个具体目标，重点是：（1）鉴定与存在或不存在轻度至中度口味疼痛相关的行为活动，这些疼痛是可靠且有效的； （2）验证行为评估检测镇痛剂量反应效应的能力； （3）使用这些新鉴定的行为措施，测试非甾体类抗炎药的功效，该药物的靶向最近据报道在存在咀嚼性肌肉疼痛的情况下被上调的特定离子通道。 该提案的总体目标是建立可靠且有效的行为喂养活动，以准确反映轻度至中度的口面痛或无痛状况。然后，这些行为参数可用于评估针对特定的口面疼痛条件的治疗干预措施的功效。对家用笼子环境中的口面疼痛行为的自动评估在昼夜和夜间都将允许更高的啮齿动物评估的吞吐量，并加快针对口腔疼痛的新疼痛疗法的翻译。 公共卫生相关性：涉及头和脸的疼痛很难在动物中测量。需要新的疼痛测量技术来评估动物的面部疼痛，以便开发出新的治疗方法。该建议将确定可用于测量轻度至中下颌肌肉疼痛的动物喂养行为。然后，这些行为将用于测试药物治疗下颌肌肉疼痛的能力。