NERVE-MUSCLE INTERACTIONS DURNIG JAW MUSCLE DEVELOPMENT

下颌肌肉发育过程中的神经肌肉相互作用

• 批准号: 6329244
• 负责人: CHARLES G. WIDMER
• 金额: $ 24.34万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6329244
• 关键词: Mammalia; bungarotoxins; cell cell interaction; cell death; cell differentiation; central nervous system; developmental neurobiology; embryo /fetus; embryo /fetus cell /tissue; facial muscles; fluorescent dye /probe; histology; image processing; immunocytochemistry; immunofluorescence technique; innervation; jaw; jaw movement; laboratory mouse; mastication; mathematical model; motor neurons; myofibrils; myogenesis; neurogenesis; neuromuscular system; phase contrast microscopy; statistics /biometry; striated muscles; tissue /cell culture; trigeminal nerve

DESCRIPTION (Adapted from investigator's Abstract): The role of nerve muscle interactions in the development and differentiation of mammalian muscles of mastication and the patterning of neuronal organization has received little attention. However, much research has concentrated on the role of nerve-muscle interactions in limb muscle development. Previous studies in the limb have determined that motoneurons and the muscles they innervate are dependent on one another for their normal development, their maintenance and their physiological properties. Although valuable clues as to the role of nerve-muscle interactions in the development and maintenance of masticatory muscles can be gleaned from studies of limb muscle, the result of limb studies may not be totally applicable. The muscles of mastication differ from limb muscle in their embryonic origin, the origin of the sensory and motor neurons innervating them and in the uniqueness of the fiber types expressed in the adult. Moreover, an understanding of developmental processes involved in orofacial morphogenesis is required for an understanding of the adult form and function, and may provide valuable insight into what limitations might be encountered in modifying the adult form. Therefore, the focus of this proposal is to investigate the temporospatial development of the masticatory muscles and the trigeminal complex to enhance our understanding of nerve-muscle interactions during development. Two hypotheses are proposed to be tested during this project and focus on trigeminal central nervous system organization, masticatory muscle development and nerve-muscle interactions. The first hypothesis is that central components of the trigeminal system involved in the innervation of the muscles of mastication including motV and mesV, develop in distinct temporospatial sequences and in response to cues from their targets. The second hypothesis is that the adult phenotype of masticatory muscle fibers and the muscle fiber distribution within each jaw muscle are dependent on trigeminal nerve-muscle interactions. These two hypotheses will be tested in three specific aims that include in vivo and in vitro investigations.

描述（根据研究者的摘要改编）：神经的作用 哺乳动物的发育和分化中的肌肉相互作用 咀嚼肌肉和神经元组织的模式 很少受到关注。 但是，许多研究集中在 神经肌肉相互作用在肢体肌肉发育中的作用。 以前的 肢体的研究确定了运动神经元和肌肉 神经关系彼此依赖于他们的正常发展，他们的 维护及其生理特性。 虽然有价值的线索是 神经肌肉相互作用在发展和维护中的作用 可以从肢体肌肉的研究中收集咀嚼肌肉 肢体研究的结果可能不完全适用。 肌肉的肌肉 咀嚼性与肢体肌肉的胚胎起源不同，起源于 感官和运动神经元支配它们，并以独特性 成人表达的纤维类型。 而且，对 需要与口面形态发生有关的发展过程 对成人形式和功能的理解，并可能提供有价值的 深入了解在修改成年人时可能会遇到什么限制 形式。 因此，该提议的重点是调查 咀嚼肌肉和三叉神经的暂时空间发育 复杂以增强我们对神经肌肉相互作用的理解 发展。 建议在此项目中检验两个假设 并专注于三叉神经中枢神经系统组织，咀嚼性 肌肉发育和神经肌肉相互作用。 第一个假设是 涉及神经系统的三叉神经系统的中心组成部分 包括MOTV和MESV在内的咀嚼肌肉，以不同的方式发展 暂时空间序列并响应其目标线索。 这 第二个假设是咀嚼肌纤维的成年表型 每个下颌肌肉内的肌肉纤维分布取决于 三叉神经肌肉相互作用。 这两个假设将被检验 在包括体内和体外研究在内的三个特定目标中。