Biofunctional sealant with peptides to extend lifespans of Class V restorations

含有肽的生物功能密封剂可延长 V 类修复体的使用寿命

基本信息

批准号：
10021400
负责人：
Nicholas G Fischer
金额：
$ 4.4万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-03-01 至 2025-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10021400
关键词：
Address Adhesions Adhesives Affinity Aging Air Amino Acids Amputees Bacteria Bacterial Infections Binding Biocompatible Materials Biological Biology Catheters Cells Chemicals Chemistry Clinical Coculture Techniques Data Dental Dental Implants Dental Schools Dentin Dentistry Devices Disease Doctor of Philosophy Dopa Elderly Elements Engineering Environment Epithelial Attachment Esthetics Extracellular Matrix Extracellular Matrix Proteins Failure Fibroblasts Flow Cytometry Formulation Gingiva Gingival Recession Goals Grant Health Care Costs Hemidesmosomes Implant Individual Integrin alpha6beta4 Integrins Knowledge Lead Learning Longevity Maintenance Mechanics Mediating Methacrylates Minnesota Modeling Modernization Molecular Biology Techniques Nature Oligopeptides Oral Oral cavity Patients Peptides Performance Periodontitis Population Prevalence Quality of life Research Research Personnel Resolution Roentgen Rays Route Scientist Series Signal Transduction Societies Spectrum Analysis Structure Surface System Techniques Technology Testing Tissues Tooth structure Training Universities Up-Regulation Water base biomaterial compatibility career career development combat design experience improved innovation keratinocyte microbial multidisciplinary novel peri-implantitis prevent response restoration restorative dentistry restorative material tooth surface

项目摘要

Class V dental restorations, those on the lower third of the tooth, are the least durable type of dental restoration and are quickly increasing in placement due to a rapidly aging society. Water and bacteria mediated destruction of the dentin/restoration interface is the main cause of failure. Maintenance of integrity between the restorative material and surrounding tissue is an unexplored route to increase lifespans of Class V restorations to shield the vulnerable adhesive interface from bacteria-mediated degradation, promote restoration stability, and increase restoration aesthetics. Tissue attachment to current Class V restorative surfaces does not occur as current restorative materials lack any activity designed to influence tissue responses. This lack of attachment reveals the vulnerable dentin/restoration interface and exacerbates Class V failure. Functional tissues near Class V restorations may be able to naturally prevent subgingival plaque and lead to longer lifespans by exploiting the tissue. Our central goal is to develop new materials and apply experimental approaches toward reducing Class V failure. In Aim 1, we aim to understand oral cellular activation responsible for signaling and test candidate molecules for their ability to survive enzymatic degradation. In Aim 2, we will demonstrate our system enables biocompatible, facile, rapid, and robust delivery of biofunctional molecules to the material surface and retains molecules through a series of challenges meant to simulate the oral environment. The results will potentially extend lifespans of Class V dental restorative materials and reduce longterm healthcare costs. This training grant will provide pivotal opportunities to learn techniques including flow cytometry, high resolution X-ray photoelectron spectroscopy, and molecular biology techniques. Improved understanding of these techniques will further my progress toward becoming an independent academic researcher at a dental school.

V 类牙齿修复体（位于牙齿下三分之一处）是最不耐用的牙齿修复体类型，并且由于社会迅速老龄化，其种植量正在迅速增加。水和细菌介导的牙本质/修复体界面破坏是失败的主要原因。保持修复材料与周围组织之间的完整性是一种尚未探索的途径，可以延长 V 类修复体的使用寿命，从而保护脆弱的粘合界面免受细菌介导的降解，提高修复体的稳定性并增加修复体的美观性。由于当前的修复材料缺乏任何旨在影响组织反应的活性，因此不会发生组织附着到当前的 V 类修复表面。这种附着的缺失暴露了牙本质/修复体界面的脆弱性，并加剧了 V 级故障。 V 类修复体附近的功能组织可能能够自然地预防龈下菌斑，并通过利用该组织来延长寿命。我们的中心目标是开发新材料并应用实验方法来减少 V 级故障。在目标 1 中，我们的目标是了解负责信号传导的口腔细胞激活，并测试候选分子在酶降解中的生存能力。在目标 2 中，我们将展示我们的系统能够将生物功能分子生物相容性、轻松、快速且稳健地递送到材料表面，并通过一系列旨在模拟口腔环境的挑战来保留分子。研究结果将有可能延长 V 类牙科修复材料的使用寿命并降低长期医疗费用。这笔培训补助金将为学习流式细胞术、高分辨率 X 射线光电子能谱和分子生物学技术等技术提供关键机会。对这些技术的更好的理解将进一步推动我成为牙科学校的独立学术研究员。