Bacterial Adhesion Inhibition and Biofilm Disruption by Adaptive Piezoelectric Biomaterial

自适应压电生物材料抑制细菌粘附和破坏生物膜

• 批准号: 10668030
• 负责人: Geelsu Hwang
• 金额: $ 20.78万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10668030
• 关键词: Acids; Address; Adhesions; Adhesives; Antimicrobial Resistance; Bacteria; Bacterial Adhesion; Barium; Binding; Biocompatible Materials; Bite Force; Candida albicans; Cells; Characteristics; Clinical; Composite Dental Resin; Data; Dental; Dental caries; Development; Electric Stimulation; Electron Transport; Electrostatics; Environment; Exhibits; Failure; Fluorescence; Future; Goals; Histologic; Homeostasis; Human; Hydrophobicity; Impairment; In Vitro; Incubated; Individual; Infection; Long-Term Effects; Mastication; Measures; Methodology; Microbe; Microbial Biofilms; Microfluidics; Modality; Modeling; Motion; Oral; Oral cavity; Outcome; Performance; Population; Prevention strategy; Property; Risk Reduction; Rodent; Rodent Model; Streptococcus gordonii; Streptococcus mutans; Surface; Surface Properties; System; Testing; Thick; Tooth Tissue; Tooth structure; Toothbrushing; Work; bactericide; biomaterial compatibility; clinical application; clinically relevant; cost comparison; cytotoxicity; design; efficacy evaluation; electrical potential; electrical property; experimental study; functional restoration; fungus; in vivo; in vivo Model; innovation; mathematical model; mechanical force; mechanical properties; metallicity; microbial; microbial colonization; microbiota; microleakage; microorganism; nanocomposite; nanoparticle; novel; oral microbial community; pathogenic bacteria; polymicrobial biofilm; prevent; real-time images; response; restoration; restorative material; zeta potential

Dental resin composites have been widely used clinically due to their bonding potential to the tooth tissues, good mechanical properties, and lower cost compared to other indirect restorations. While successful, long-term survival of a restoration can be compromised by secondary caries at the tooth-composite margins. In most cases, failure is due to the microleakage of bacteria and their acid by-products through the margins between composite and tooth structures. Once biofilms are established on a surface, it is extremely difficult to remove or kill pathogenic bacteria therein. Therefore, inhibition of microbial adhesion or inactivation of the adhered bacteria could impair their development into biofilms. The goal of this application is to create a novel dental composite that inhibits biofilm accumulation as well as dislodging surface-adhered microbes on restorative materials using enhanced electric potential at the interface generated by oral motion without relying on microbial killing activity. A nanocomposite platform based on barium titanate (BaTiO3) nanoparticles enables antibiofilm and self-powering functionalities for biomedical applications. This nanocomposite surface inhibits bacterial colonization by utilizing its intrinsic physicochemical properties without bactericidal activity, thereby minimizing the induction of antimicrobial resistance and destruction of homeostasis microbiota. In addition, the piezoelectric property of BaTiO3 nanoparticles that converts normal oral motions into electrical energy can be utilized to enhance its antibiofilm activity. Ongoing studies indicate that antibiofilm activity can be further enhanced by modulating the work function by introducing a shallow metallic surface (< 100 Å) on the nanocomposite, exhibiting almost complete inhibition of bacterial colonization. Based on this exciting supporting data, we hypothesize that force- powering of piezoelectric crystals to produce enhanced electric potential combined with bacterial anti-adhesive property creates an anti-infectious environment that prevents the development of biofilms on restorations and secondary caries. We anticipate that the creation of this anti-infectious smart biomaterial would increase the functionality of restorations and provide a new strategy to prevent secondary caries as well as reduce the risk of restoration failure.

牙科树脂复合材料由于其与牙齿组织的粘接潜力、良好的粘接性能而在临床上得到了广泛的应用。 与其他间接修复相比，机械性能更高，成本更低，同时成功且长期。 在大多数情况下，修复体的存活可能会因牙齿复合材料边缘的继发龋而受到影响。 失效是由于细菌及其酸副产物通过复合材料之间的边缘微泄漏造成的。 一旦生物膜在表面形成，就很难去除或杀死。 因此，抑制微生物的附着或使附着的细菌失活。 可能会损害它们形成生物膜的能力。该应用的目标是创造一种新型牙科复合材料。 抑制生物膜积累并去除修复材料表面粘附的微生物 通过口腔运动产生的界面处增强的电势，而不依赖于微生物杀灭活性。 基于钛酸钡 (BaTiO3) 纳米粒子的纳米复合材料平台可实现抗生物膜和自供电 这种纳米复合材料表面利用生物医学应用的功能来抑制细菌定植。 其固有的理化特性没有杀菌活性，从而最大限度地减少了细菌的诱导 抗菌素耐药性和微生物群稳态的破坏此外，压电特性。 BaTiO3 纳米粒子可将正常口腔运动转化为电能，可用于增强口腔运动 正在进行的研究表明，可以通过调节抗生物膜活性来进一步增强。 通过在纳米复合材料上引入浅金属表面（< 100 Å）来实现功函数，几乎表现出 基于这个令人兴奋的支持数据，我们采取了这种力量- 压电晶体供电以产生增强的电势并结合细菌抗粘附剂 财产创造了一个抗感染环境，防止修复体和生物膜的形成 我们预计这种抗感染智能生物材料的创建将增加继发性龋齿的发生率。 修复体的功能，并提供预防继发龋和降低龋齿风险的新策略 恢复失败。