Multi-modality optical imaging of single-cell dynamics using supercontinuum light source

使用超连续谱光源的单细胞动力学多模态光学成像

基本信息

批准号：
10798646
负责人：
Xuan Liu
金额：
$ 8.17万
依托单位：
NEW JERSEY INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-01-01 至 2025-12-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10798646
关键词：
3-Dimensional Address Adhesions Adhesives Administrative Supplement Affect Award Biomedical Research Budgets Cell Adhesion Cell Survival Cells Characteristics Clinical Research Collimator Complex Contrast Media Detection Disease Early Diagnosis Extracellular Matrix Fluorescence Fluorescence Microscopy Fourier Transform Functional disorder Funding Future Halogens Image Imaging technology Infrastructure Interferometry Investigation Knowledge Lasers Lead Light Lighting Mechanics Methods Microscopy Molecular Monitor Motion Multi-modal optical imaging Multimodal Imaging Noise Optics Organelles Output Parents Patients Phase Photons Play Process Proliferating Property Research Research Project Grants Resolution Role Signal Transduction Source Specimen Structure Subcellular structure System Xenon biomaterial development cell behavior cellular imaging detector flexibility fluorescence imaging fluorophore fundamental research imaging platform imaging study imaging system improved light emission meter microscopic imaging migration nanoscale photon-counting detector photonics preclinical study prototype quantitative imaging technology development

项目摘要

Project Summary The objective of this study is to develop a multi-modality imaging platform that integrates complex phase microscopy (CPM) based on optical computation and fluorescence microscopy (FM). We will use the imaging platform to correlate the sub-cellular dynamic motion and intracellular properties to study cell adhesion which plays an important role in many aspects of cell behavior. This study is significant for fundamental research, preclinical and clinical study, and technology development. By simultaneously imaging cell motion and organelle properties, CPM-FM enables the investigation of cell dynamics in correlation biomolecular characteristics. CPM-FM investigation of cell adhesion will result in better understanding of pathophysiology of different diseases, open the door to new methods for disease treatment and early diagnosis, lead to the development biomaterials that need specific interaction with cells, and benefit patients and clinicians. This study will also establish the feasibility of CPM as an imaging technology that allows non-invasive, and continuous monitoring of cell activities without contrast agents. Particularly, a supercontinuum light source with broad bandwidth, high spatial coherence and large output power will be acquired and used as the light source for the imaging system, to achieve nanoscale displacement sensitivity, sub-micrometer level spatial resolution and rich molecular information from multiple fluorescence probes. The acquisition of the powerful light source is expected to enhance our research capability significantly.

项目概要本研究的目的是开发一个集成了多模态成像平台基于光学计算和荧光显微镜的复杂相位显微镜（CPM）（调频）。我们将使用成像平台将亚细胞动态运动与细胞内特性来研究细胞粘附，这在许多方面发挥着重要作用细胞行为。该研究对于基础研究、临床前和临床研究具有重要意义，和技术开发。通过同时成像细胞运动和细胞器特性， CPM-FM 能够研究相关生物分子特征的细胞动力学。 CPM-FM 对细胞粘附的研究将有助于更好地了解细胞粘附的病理生理学不同的疾病，为疾病治疗和早期诊断的新方法打开了大门，导致需要与细胞发生特定相互作用的生物材料的开发，并受益患者和临床医生。这项研究还将确定 CPM 作为成像技术的可行性无需对比即可非侵入性、连续监测细胞活动的技术代理。特别是具有宽带宽、高空间的超连续谱光源将获得相干性和大输出功率并用作成像光源系统，实现纳米级位移灵敏度、亚微米级空间分辨率以及来自多个荧光探针的丰富分子信息。收购强大的光源有望显着提高我们的研究能力。