Monkey Alcohol Tissue Research Resource (MATRR)

猴子酒精组织研究资源 (MATRR)

基本信息

批准号：
10019779
负责人：
Erich Baker
金额：
$ 52.94万
依托单位：
OREGON HEALTH & SCIENCE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-20 至 2025-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10019779
关键词：
Abstinence Adult Affect Alcohol consumption Alcohols Archives Autopsy Award Bioinformatics Biological Models Biopsy Blood Categories Chronic Collaborations Collection Communities Custom Data Data Analyses Data Analytics Data Set Development Developmental Process Dextrins Diagnostic Discipline Disease Disease Progression Elasticity Endocrine Endocrinology Equipment and supply inventories Ethanol Evaluation Experimental Designs Fatty acid glycerol esters Female Fetal Alcohol Exposure Fetal Tissues Foundations Freezing Functional disorder Funding Generations Genetic Genetic study Genomics Goals Grant Harvest Health Sciences Heavy Drinking Histologic Human Image Immune Immunology In Vitro Individual Institutes Intake Investigation Knowledge Laboratories Liver Macaca Macaca mulatta Magnetic Resonance Imaging Maltose Medical Modeling Monkeys Neuraxis Neuroanatomy Neurosciences Oregon Organ Pathologic Pathology Pattern Peer Review Phase Phenotype Physiology Positioning Attribute Preparation Primates Procedures Process Progress Reports Protocols documentation Publications Relapse Reproducibility Research Research Personnel Resources Risk Roentgen Rays Sampling Self Administration Severities Ships Structure System Testing Time Tissue Sample Tissues Ultrasonography Universities Variant adverse outcome alcohol consequences alcohol research alcohol use disorder base bioinformatics resource bone brain behavior chronic alcohol ingestion cohort comorbidity data integration design drinking expectation fetal genetic pedigree genetic signature genomic signature heterogenous data in silico in vivo imaging interest large scale data longitudinal design male nonhuman primate novel online resource recruit response screening tissue preparation underage drinking web based interface web site

项目摘要

PROJECT SUMMARY The “Monkey Alcohol Tissue Research Resource” (MATRR) is a multiple PI research resource award from Oregon Health & Science University and Baylor University. The MATRR has been highly successful in providing the alcohol research community access to tissue and data generated from cohorts of monkeys that have completed a standard protocol to study voluntary alcohol self-administration. The procedure results in a wide distribution of average daily ethanol intakes (g/kg) and the MATRR bioinformatics has established reliable, robust categories of drinking that encompass low, binge, heavy and very heavy chronic intake patterns. The necropsy procedure is fully refined and allows for the highest quality harvesting of all organs and tissues in the body. Prior to necropsy, registered users and other interested parties are contacted for custom tissue preparations, otherwise frozen and fixed tissue remain in the MATRR until requests are made through a dedicated web resource. All tissues are accompanied by data on organismal and alcohol intake variables to aid in rapid publication of findings. Importantly, the MATRR provides an opportunity for laboratories with specific expertise in organ pathology, neuroscience and genomics the ability to extend their research to a primate model with similar genetics, developmental processes, endocrinology, immunology, physiology and neuroanatomy to humans. Thus, the MATRR is unique to the alcohol research field in promoting a translational resource for mechanistic studies of alcohol-induced pathologies. The MATRR resource has exceeded all expectations in terms of tissue generation, inventory control, tissue requests, tissue utilization, data analytics, novel data generated, publications, newly funded projects and new research directions. In this renewal, we propose to add 3 more cohorts, with an expected accumulation of tissues and data from 20 cohorts of monkeys (total n=240 (162 ethanol, 78 control)). This has positioned the MATRR to fill experimental designs requiring large number of individuals, for example genetic and in vivo imaging datasets. The inventory control is aided by a unique web-based interface that also serves to administratively evaluate and act upon tissue requests. In total, we have 226 registered users and have shipped over 3,813 tissue samples from 5,590 tissue and data requests. With each accepted request an MTA is filed and timely progress reports are automatically requested. In the past funding, the resource has been extremely successful in the number of publications (48 in 48 months) from 22 different laboratories at 13 unique universities or Institutes. There remain great opportunities in further building and refining this resource. Our goal is to have the MATRR bioinformatics integrate data across disciplines, organs and individuals to generate knew knowledge of chronic alcohol use disorders. Finally, we acknowledge that in addition to building critical numbers for large scale data analyses, the opportunity exists to study additional tissue (such as fetal), variations in the protocol (such as necropsy in abstinence) and additional datasets such as sonography for liver elasticity.

项目概要 “猴子酒精组织研究资源”(MATRR) 是多项 PI 研究资源奖俄勒冈健康与科学大学和贝勒大学在这方面取得了巨大成功。为酒精研究界提供从猴子群体中生成的组织和数据已完成研究自愿酒精自我管理的标准方案该程序的结果是平均每日乙醇摄入量 (g/kg) 分布广泛，并且 MATRR 生物信息学已建立可靠、稳健的饮酒类别，包括低量、暴饮、大量和非常大量的长期摄入量尸检程序经过全面完善，可以最高质量地采集所有器官和器官。在尸检之前，会联系注册用户和其他感兴趣的各方进行定制。组织制剂，否则冷冻和固定的组织将保留在 MATRR 中，直到通过所有组织均附有生物和酒精摄入变量数据以提供帮助。重要的是，MATRR 为具有特定特征的实验室提供了机会。器官病理学、神经科学和基因组学方面的专业知识能够将他们的研究扩展到灵长类动物具有相似遗传学、发育过程、内分泌学、免疫学、生理学和因此，MATRR 在促进酒精研究领域的转化方面是独一无二的。酒精引起的病理机制研究的资源 MATRR 资源超过了所有资源。对组织生成、库存控制、组织请求、组织利用、数据分析等方面的期望在这次更新中，我们产生了新的数据、出版物、新资助的项目和新的研究方向。建议再添加 3 个队列，预计会积累 20 个队列的组织和数据猴子（总数 n=240（162 只乙醇，78 只对照）），这使 MATRR 能够满足实验设计。需要大量个体，例如遗传和体内成像数据集库存控制。借助独特的基于网络的界面，该界面还可以对组织进行管理评估和作用我们总共有 226 个注册用户，并已从 5,590 个组织中运送了超过 3,813 个组织样本。对于每个接受的请求，都会提交 MTA，并自动及时生成进度报告。在过去的资助中，该资源在出版物数量方面非常成功（48）。 48 个月内）来自 13 所独特大学或研究所的 22 个不同实验室。我们的目标是拥有 MATRR 生物信息学。整合跨学科、器官和个人的数据，生成有关慢性酒精使用的已知知识最后，我们承认除了为大规模数据分析建立关键数字之外，有机会研究额外的组织（例如胎儿）、方案的变化（例如尸检）禁欲）和其他数据集，例如肝脏弹性超声检查。