Monkey Alcohol Tissue Research Resource (MATRR)

猴子酒精组织研究资源 (MATRR)

基本信息

批准号：
9752948
负责人：
Erich Baker
金额：
$ 51.33万
依托单位：
OREGON HEALTH & SCIENCE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-20 至 2020-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9752948
关键词：
Address Affect Age Alcohol consumption Alcohols Animal Organ Archives Autopsy Award Bioinformatics Brain Cercopithecidae Chronic Collaborations Collection Communities Custom Data Data Analyses Data Analytics Data Set Development Developmental Process Discipline Disease Electrophysiology (science)Endocrine Endocrinology Equipment and supply inventories Ethanol Evaluation Fetal Alcohol Exposure Fetal Alcohol Spectrum Disorder Fetus Foundations Freezing Functional Imaging Funding Generations Genes Genetic Genetic study Genomics Goals Harvest Health Sciences Heavy Drinking Human Immune Immunology In Vitro Individual Intake Knowledge Laboratories Macaca fascicularis Macaca mulatta Magnetic Resonance Imaging Management Information Systems Medical Modeling Molecular Monkeys Mothers Neuraxis Neuroanatomy Neurosciences Oregon Organ Pathologic Pathology Pathway Analysis Pattern Peripheral Phenotype Physiological Physiology Pregnancy Preparation Primates Principal Investigator Procedures Process Progress Reports Protocols documentation Publications Research Research Personnel Resources Rest Risk Rodent Self Administration Site Slice Social status Testing Time Tissue Banks Tissue Sample Tissues Universities adverse outcome alcohol consequences alcohol exposure alcohol research alcohol use disorder behavioral pharmacology bioinformatics resource bone brain behavior chronic alcohol ingestion cohort data integration data resource design drinking drinking onset expectation forest genetic analysis in vivo imaging indexing interest longitudinal design neurochemistry neuroimaging non-invasive imaging nonhuman primate novel online resource public health relevance response sex web based interface web site

项目摘要

DESCRIPTION (provided by applicant): The "Monkey Alcohol Tissue Research Resource" (MATRR) is a multiple PI research resource award from Oregon Health & Science University, Baylor University, and Wake Forest Health Science. The MATRR was established to provide the alcohol research community access to tissue and data generated from cohorts of monkeys that have been subjected to the same voluntary alcohol self-administration protocol. In this procedure, monkeys show a wide distribution of average daily ethanol intakes (g/kg) encompassing low, binge, heavy and very heavy chronic intake patterns. Our necropsy procedure is fully refined and allows for the harvesting of brain slices that are rapidly frozen or prepared for ex vivo electrophysiological and neurochemical analyses. Prior to necropsy, registered users and other interested parties are contacted for any custom preparations, otherwise the frozen tissue remain in the MATRR until requests are made through a dedicated web resource. All tissues are accompanied by organismal and alcohol intake data to aid in rapid publication of findings. Importantly, the MATRR provides an opportunity for laboratories with specific expertise in neuroscience, organ pathology, and genetic analyses the ability to extend their research to a primate model with similar genetics, developmental processes, endocrinology, immunology, physiology and neuroanatomy to humans. Thus, the MATRR is unique to the alcohol research field in promoting a translational resource for mechanistic studies of alcohol-induced pathologies. The MATRR resource has exceeded all expectations in terms of tissue generation, inventory control, tissue requests, tissue utilization, data analytics, novel daa generated, publications, newly funded projects and new research directions. In terms of tissue generation, currently there are tissues and data from 13 cohorts of monkeys available for request. In this renewal, we propose to add 10 more cohorts, bringing the total number of monkey to 248 (182 ethanol, 66 control) to allow a critical number for genetic and neuroimaging datasets. The inventory control has been aided by a unique web-based interface that also serves to administratively evaluate and act upon tissue requests. We have provided over 2,100 tissue samples, with MTAs filed prior to sending and timely progress reports automatically requested. The resource has been extremely successful in the number of publications (42) and funded applications (33) from 26 Principal Investigators at 11 sites. There remain great opportunities in further building and refining this resource. Our goal is to have the MATRR bioinformatics integrate data across disciplines, organs and individuals to generate knew knowledge of chronic alcohol use disorders. Finally, we acknowledge that in addition to building critical numbers for large-scale data analyses, the opportunity exists to study additional tissue, such as fetal alcohol exposure, and additional datasets such as resting state functional imaging MRI.

描述（由申请人提供）：“猴子酒精组织研究资源”（MATRR）是由俄勒冈健康与科学大学、贝勒大学和维克森林健康科学学院颁发的多项 PI 研究资源。 MATRR 的建立是为了提供酒精研究。社区获取接受相同自愿酒精自我给药方案的猴子群体的组织和数据。在此过程中，猴子的平均每日乙醇摄入量（克/千克）分布广泛，包括：低、暴饮暴食、大量和非常大量的慢性摄入模式我们的尸检程序经过充分完善，可以采集快速冷冻或的脑切片。在尸检之前，会联系注册用户和其他感兴趣的各方进行任何定制准备，否则冷冻组织将保留在 MATRR 中，直到通过专用网络资源提出请求。重要的是，MATRR 为在神经科学、器官病理学和遗传分析方面具有特定专业知识的实验室提供了一个机会，使其能够将其研究扩展到具有相似遗传学、发育的灵长类动物模型。因此，MATRR 在促进酒精引起的病理机制研究的转化资源方面是酒精研究领域所独有的。库存控制、组织请求、组织利用、数据分析、新的 daa 生成、出版物、新资助的项目和新的研究方向在组织生成方面，目前有来自 13 组猴子的组织和数据。在这次更新中，我们建议再增加 10 个队列，使猴子总数达到 248 只（182 只乙醇，66 只对照），以便为遗传和神经影像数据集提供关键数量的库存控制。基于界面，还可以对组织请求进行管理评估和采取行动。我们已经提供了 2,100 多个组织样本，并在发送之前提交了 MTA，并自动请求了及时的进度报告。 11 个地点的 26 位主要研究人员发表了 42 篇出版物，并资助了 33 篇申请。我们的目标是让 MATRR 生物信息学整合跨学科、器官和个人的数据来生成。最后，我们承认，除了为大规模数据分析建立关键数据之外，还有机会研究其他组织（例如胎儿酒精暴露）和其他数据集（例如静息状态功能成像）。核磁共振成像。