Sex Specific Effects of Adolescent Alcohol Exposure on BNST Plasticity

青少年酒精暴露对 BNST 可塑性的性别特异性影响

• 批准号: 10836173
• 负责人: Tiffany A Wills
• 金额: $ 3.64万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-15 至 2026-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10836173
• 关键词: Acute; Adolescence; Adolescent; Adult; Alcohol consumption; Alcohol withdrawal syndrome; Alcohols; American; Amygdaloid structure; Anxiety; Area; Bachelor' s Degree; Behavior; Behavioral; Brain; Brain region; Cell Nucleus; Data; Development; Disparity population; Electrophysiology (science); Eligibility Determination; Environment; Female; Freezing; Funding; Future; Glutamates; Goals; Grant; Hippocampus; Hypothalamic structure; Legal Guardians; Low income; LoxP-flanked allele; Male Adolescents; Mediating; Mental Depression; Mus; Neurons; Neurotransmitters; Output; Parents; Phenotype; Physiological; Positioning Attribute; Postdoctoral Fellow; Qualifying; Recording of previous events; Relapse; Research; Sex Differences; Shock; Signal Transduction; Stress; Structure; Structure of terminal stria nuclei of preoptic region; Synaptic Transmission; System; Technical Expertise; Testing; Training; United States Centers for Medicare and Medicaid Services; Vietnamese; Withdrawal; Work; adolescent alcohol effect; adolescent alcohol exposure; alcohol abstinence; alcohol exposure; alcohol relapse; alcohol research; alcohol testing; alcohol use disorder; alcoholism therapy; behavioral response; biological adaptation to stress; designer receptors exclusively activated by designer drugs; disadvantaged background; experience; foot; graduate student; health professional shortage areas; innovation; job market; kappa opioid receptors; male; mouse model; negative affect; paraventricular nucleus; parent grant; presynaptic; prevent; programs; relapse prevention; restraint stress; sex; sexual dimorphism; skill acquisition; stressor; underage drinking

Summary/Abstract: Linh Ha is a phenomenal first-year graduate student that will be joining my lab in May 2023. She is a Vietnamese American that grew up in New Orleans, LA. Linh qualifies for this diversity supplement under the “Individuals from disadvantaged backgrounds” and meets four of the seven criteria including: 1) have/had no parents or legal guardians who completed a bachelor’s degree, 2) were or currently are eligible for Federal Pell grants, 3) were eligible for the Federal Free and Reduced Lunch Program for two or more years, and 4) grew up in a “Centers for Medicare and Medicaid Services-designated Low-Income and Health Professional Shortage Areas”. The goal of the funded parent grant, R01AA028011-01A1, is to investigate the long-term sex-dependent changes in BNST plasticity and negative affect behavior from adolescence alcohol exposure and adult stress. Specifically, it evaluates the sex-dependent impacts of adolescent alcohol and stress on 1) the enhancement of glutamatergic inputs [via CRF-CRFR1 signaling], 2) BNST plasticity in specific projection regions, and 3) negative affect behavior. This diversity supplement describes a research plan that will extend this work to test the overarching hypothesis that BNST-kappa opioid receptors (KORs) regulate the sex differences in BNST plasticity and negative affect from adolescent alcohol exposure and stress. BNST-KORs are known to be important in alcohol withdrawal, stress, and negative affect but the interaction of these factors has not been explored in the context of adolescent alcohol exposure or across sex. The technical skills utilized in this research plan (especially electrophysiology) will set Linh apart when entering the job market for a postdoc position. LSUHSC is also great environment for graduate training and will allow Linh to gain expertise in alcohol research, professional development skills, grantsmanship training, and opportunities to share her work.

摘要/摘要：Linh Ha 是一名出色的一年级研究生，她将加入我的团队 2023 年 5 月在实验室工作。她是一名越南裔美国人，在洛杉矶新奥尔良长大，符合资格。 对于“来自弱势背景的个人”下的这种多样性补充，并满足 七个标准中的四个包括：1）没有父母或法定监护人完成了 学士学位，2) 曾经或现在有资格获得联邦佩尔助学金，3) 有资格获得 联邦免费和减少午餐计划持续两年或两年以上，并且 4) 在“中心”中长大 针对医疗保险和医疗补助服务指定的低收入和健康专业人员短缺 资助的家长补助金 R01AA028011-01A1 的目标是调查长期情况。 BNST 可塑性的性别依赖性变化和青春期酒精的负面影响行为 具体来说，它评估了青少年的性别依赖性影响。 酒精和压力对 1) 谷氨酸输入的增强 [通过 CRF-CRFR1 信号传导], 2) BNST 在特定投影区域的可塑性，以及 3）这种多样性的负面影响。 补充描述了一项研究计划，该计划将扩展这项工作以测试总体假设 BNST-kappa 阿片受体 (KOR) 调节 BNST 可塑性的性别差异 众所周知，青少年酒精暴露和压力会产生负面影响。 对酒精戒断、压力和负面影响很重要，但这些因素的相互作用 尚未在青少年酒精暴露或跨性别的背景下进行探讨。 本研究计划中使用的技能（尤其是电生理学）将使 Linh 在进入时脱颖而出 路易斯安那州立大学HSC 的就业市场也是研究生培训的良好环境。 并将让 Linh 获得酒精研究方面的专业知识、专业发展技能、 资助培训，以及分享她的工作的机会。