Physiological and Molecular Imaging

生理和分子成像

基本信息

批准号：
7753056
负责人：
Tanya N Mayadas
金额：
$ 18.94万
依托单位：
BRIGHAM AND WOMEN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-07-01 至 2014-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7753056
关键词：
Affect Animals Antibodies Arts Binding Biology Blood Vessels Blood flow Bone Marrow Cell Communication Cells Computer-Assisted Image Analysis Data Analyses Development Dorsal Ear Endothelium Equipment Fluorescence Functional disorder Goals Hour Human Resources Image Immersion Investigative Technique In Vitro Individual Instruction Knowledge Label Laboratories Lamina Propria Life Mesentery Microcirculation Microscope Modeling Muscle Operative Surgical Procedures Organ Peripheral Physiological Preparation Productivity Proteins Research Research Personnel Resources Saline Services Skin Structure of aggregated lymphoid follicle of small intestine Structure of jugular vein System Technical Expertise Techniques Time Training Work base blood perfusion cellular imaging cremaster muscle data acquisition femoral artery in vivo instrument intravenous injection intravital microscopy lens lymph nodes molecular imaging programs tissue preparation training project

项目摘要

The goal of this Core is to provide "real fime" physiological and molecular imaging support for various experimental applicafions in this Program. The Core will provide equipment and expertise for the qualitative and quantitafive analysis of cellular and molecular interactions at two different levels: cell-cell and cellmolecular interactions in vitro, and cell-cell interactions in vivo in the context of the vessel wall. The in vitro component of this Core will give Pis access to live cell imaging capabilifies, and computer assisted image analysis, of unlabeled cells, or cells labeled with fluorescently tagged antibodies or transduced to express GFP-tagged protein. This component is referred to as the Live cell fluorescence Microscopv facility. For in vivo studies, the established Intravital Microscopv (IVM) facility in The Center for Excellence in Vascular Biology will make available state-of the art capabilities for real-time imaging ofthe microcirculation in anesthetized animals. Thus through this Core, Program investigators will leverage resources that would be too expensive for single laboratories to purchase and maintain. Importanfiy, the Core will provide expertise and technical assistance to increase the productivity of individual projects as well as increase the capabilities of the Pis to accomplish the experimental aims of each project.

该核心的目标是为各种疾病提供“实时”生理和分子成像支持本程序中的实验应用。该核心将为定性提供设备和专业知识以及两个不同水平上细胞和分子相互作用的定量分析：细胞-细胞和细胞分子体外相互作用，以及体内血管壁中细胞与细胞的相互作用。体外该核心的组件将使 Pis 能够访问活细胞成像功能和计算机辅助图像分析未标记的细胞或用荧光标记抗体标记的细胞或转导表达的细胞 GFP 标记的蛋白质。该组件被称为活细胞荧光显微镜设施。对于体内研究中，在血管生物学卓越中心建立的活体显微镜 (IVM) 设施将提供最先进的麻醉动物微循环实时成像功能。因此，通过这个核心，计划研究人员将利用对于单个人来说过于昂贵的资源实验室购买和维护。重要的是，核心将提供专业知识和技术援助提高单个项目的生产力以及提高 Pi 完成任务的能力每个项目的实验目标。