Peromyscus Laboratory Models for Biomedical Research

用于生物医学研究的 Peromyscus 实验室模型

基本信息

批准号：
7630827
负责人：
Michael Ray Felder
金额：
$ 22.49万
依托单位：
UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-07-01 至 2014-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7630827
关键词：
Adolescent Adult American Animal Experimentation Animal Model Animals Area Ataxia Babesiosis Basic Science Behavioral Biomedical Research Bioterrorism Breeding Cell division Chromosome Mapping Chromosome Painting Chromosomes Collaborations Communicable Diseases Communities Cryopreservation Data Deer Mouse Derivation procedure Detection Development Disease EST Library Ehrlichiosis Embryo Embryo Transfer Embryonic Development Environment Epidemiology Epilepsy Expressed Sequence Tags Freezing Future Gene Expression Generations Genetic Genetic Variation Genome Genomic Imprinting Genomics Goals Grant Habitats Hormonal Human Hybrids Individual Information Resources Institutes Internet Investigation Joints Laboratories Laboratory Organism Large-Scale Sequencing Letters Life Lyme Disease Methods Microarray Analysis Modeling Modification Movement Disorders Mus National Human Genome Research Institute Paper Peromyscus Physiological Physiological Adaptation Physiology Principal Investigator Production Publications Rattus Research Research Personnel Resources Rodent Single Nucleotide Polymorphism Social Behavior Source Study models Syndrome Technology Universities Virus Work Xenobiotic Metabolism base cost environmental toxicology gene discovery genetic linkage genetic manipulation interest medical schools microorganism mortality mutant programs public health relevance tool

项目摘要

DESCRIPTION (provided by applicant): The Peromyscus Genetic Stock Center (PGSC) provides a reliable source of disease free peromyscines, and is unmatched for the variety of Peromyscus species and mutants available. The PGSC provides animals for a variety of fields, including human infectious disease, behavioral studies, physiological adaptation, and genetic studies. Two developments should further enhance the interest in Peromyscus as an experimental organism. The NHGRI has selected, based on our submission of a white paper, to do a 6X sequence of one species and 2X sequence of 3 other species. This project is just underway at Baylor Medical College. In collaboration with Dr. Glenn (U. Georgia) a request was made to JGI/DOE for construction and sequencing of 50,000 Peromyscus EST clones. The EST clones have been made and quality assessments completed. This complete sequence data should be available within approximately one month. In addition, a first generation linkage map has revealed closer linkage relationships between Peromysus and Rattus than Peromyscus and Mus. Further linkage and chromosome painting analysis has allowed the construction of a linkage map that is chromosome based. These resources will enhance the importance of Peromyscus as a research tool in areas of gene discovery in the area of repetitive movement disorders, juvenile and adult ataxia, and epilepsy, gene expression in disease and environmental toxicology, and mechanisms of genomic imprinting, Peromyscus is a reservoir for two emerging infectious microorganisms. Hanta virus causes a disease with 40 percent mortality and is a potential bioterrorism agent. The causative agent of Lyme disease is also harbored in Peromyscus and nearly 15,000 cases occur annually. PGSC has provided animals for investigators studying these and other diseases such as Ehrlichiosis, babesiosis and plaque. The research aspect of the project is to continue the development of embryo cryopreservation methods and re- derivation of living animals in order to preserve rare mutants and germplasm for future use when needed and to decrease costs. Progress has been in obtaining an increase in embryo production using modifications of the hormonal treatment regimes used in mice. Embryos have been successfully frozen and thawed to undergo cell division. Future work will further refine these methods with the goal of re-derivation of living animals from frozen embryos. PUBLIC HEALTH RELEVANCE (provided by applicant): As a species, Peromyscus naturally occurs in diverse habitats offering unique models for studying genetic variation in relation to adaptation to particular environments. Naturally occurring mutants being maintained offer the opportunity to discover genes involved in several mutants of biomedical significance including two movement disorders. Peromyscus in the wild is a reservoir for two emerging infectious microorganisms.

描述（由申请人提供）：Peromyscus 遗传库存中心 (PGSC) 提供了无病 Peromyscine 的可靠来源，并且在 Peromyscus 物种和突变体的多样性方面是无与伦比的。 PGSC 为各个领域提供动物，包括人类传染病、行为研究、生理适应和遗传研究。两项进展应该会进一步增强人们对白鼠属作为实验生物的兴趣。根据我们提交的白皮书，NHGRI 选择对一个物种进行 6X 测序，对其他 3 个物种进行 2X 测序。该项目刚刚在贝勒医学院进行。与 Glenn 博士（美国佐治亚州）合作，向 JGI/DOE 提出了构建 50,000 个 Peromyscus EST 克隆并进行测序的请求。 EST 克隆已完成并完成质量评估。这一完整的序列数据应在大约一个月内提供。此外，第一代连锁图谱揭示了白鼠属和鼠属之间的连锁关系比白鼠属和鼠属之间的连锁关系更紧密。进一步的连锁和染色体绘制分析允许构建基于染色体的连锁图谱。这些资源将增强 Peromyscus 作为重复运动障碍、青少年和成人共济失调、癫痫、疾病和环境毒理学中的基因表达以及基因组印记机制等基因发现领域的研究工具的重要性。两种新兴传染性微生物的储存库。汉塔病毒引起的疾病死亡率为 40%，是一种潜在的生物恐怖主义媒介。莱姆病的病原体也存在于白腹鼠属中，每年发生近 15,000 例病例。 PGSC 为研究这些疾病以及埃利希体病、巴贝斯虫病和斑块等其他疾病的研究人员提供了动物。该项目的研究方面是继续开发胚胎冷冻保存方法和活体动物再衍生，以保存稀有突变体和种质以供将来需要时使用并降低成本。通过修改小鼠的激素治疗方案，在增加胚胎产量方面取得了进展。胚胎已成功冷冻和解冻以进行细胞分裂。未来的工作将进一步完善这些方法，目标是从冷冻胚胎中重新衍生出活体动物。公共健康相关性（由申请人提供）：作为一种物种，白鼠自然地生活在不同的栖息地中，为研究与适应特定环境相关的遗传变异提供了独特的模型。维持的自然发生的突变体提供了发现涉及几种具有生物医学意义的突变体（包括两种运动障碍）的基因的机会。野生的白鼠属是两种新出现的传染性微生物的储存库。