Peromyscus Laboratory Models for Biomedical Research

用于生物医学研究的 Peromyscus 实验室模型

• 批准号: 8244999
• 负责人: Michael Ray Felder
• 金额: $ 23.93万
• 依托单位: UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8244999
• 关键词: Adolescent; Adult; American; Animal Experimentation; Animal Model; Animals; Area; Ataxia; Babesiosis; Basic Science; Behavioral; Biomedical Research; Bioterrorism; Breeding; Cell division; Chromosome Mapping; Chromosome Painting; Chromosomes; Collaborations; Communicable Diseases; Communities; Cryopreservation; Data; Deer Mouse; Derivation procedure; Detection; Development; Disease; EST Library; Ehrlichiosis; Embryo; Embryo Transfer; Embryonic Development; Environment; Epidemiology; Epilepsy; Expressed Sequence Tags; Freezing; Future; Gene Expression; Generations; Genetic; Genetic Variation; Genome; Genomic Imprinting; Genomics; Goals; Grant; Habitats; Hormonal; Human; Hybrids; Individual; Institutes; Internet; Investigation; Joints; Laboratories; Laboratory Organism; Large-Scale Sequencing; Letters; Life; Lyme Disease; Methods; Microarray Analysis; Modeling; Modification; Movement Disorders; Mus; National Human Genome Research Institute; Paper; Peromyscus; Physiological; Physiological Adaptation; Physiology; Principal Investigator; Production; Publications; Rattus; Research; Research Personnel; Resources; Rodent; Single Nucleotide Polymorphism; Social Behavior; Source; Study models; Syndrome; Technology; Universities; Virus; Work; Xenobiotic Metabolism; base; cost; environmental toxicology; gene discovery; genetic linkage; genetic manipulation; interest; medical schools; microorganism; mortality; mutant; programs; public health relevance; tool

DESCRIPTION (provided by applicant): The Peromyscus Genetic Stock Center (PGSC) provides a reliable source of disease free peromyscines, and is unmatched for the variety of Peromyscus species and mutants available. The PGSC provides animals for a variety of fields, including human infectious disease, behavioral studies, physiological adaptation, and genetic studies. Two developments should further enhance the interest in Peromyscus as an experimental organism. The NHGRI has selected, based on our submission of a white paper, to do a 6X sequence of one species and 2X sequence of 3 other species. This project is just underway at Baylor Medical College. In collaboration with Dr. Glenn (U. Georgia) a request was made to JGI/DOE for construction and sequencing of 50,000 Peromyscus EST clones. The EST clones have been made and quality assessments completed. This complete sequence data should be available within approximately one month. In addition, a first generation linkage map has revealed closer linkage relationships between Peromysus and Rattus than Peromyscus and Mus. Further linkage and chromosome painting analysis has allowed the construction of a linkage map that is chromosome based. These resources will enhance the importance of Peromyscus as a research tool in areas of gene discovery in the area of repetitive movement disorders, juvenile and adult ataxia, and epilepsy, gene expression in disease and environmental toxicology, and mechanisms of genomic imprinting, Peromyscus is a reservoir for two emerging infectious microorganisms. Hanta virus causes a disease with 40 percent mortality and is a potential bioterrorism agent. The causative agent of Lyme disease is also harbored in Peromyscus and nearly 15,000 cases occur annually. PGSC has provided animals for investigators studying these and other diseases such as Ehrlichiosis, babesiosis and plaque. The research aspect of the project is to continue the development of embryo cryopreservation methods and re- derivation of living animals in order to preserve rare mutants and germplasm for future use when needed and to decrease costs. Progress has been in obtaining an increase in embryo production using modifications of the hormonal treatment regimes used in mice. Embryos have been successfully frozen and thawed to undergo cell division. Future work will further refine these methods with the goal of re-derivation of living animals from frozen embryos. PUBLIC HEALTH RELEVANCE (provided by applicant): As a species, Peromyscus naturally occurs in diverse habitats offering unique models for studying genetic variation in relation to adaptation to particular environments. Naturally occurring mutants being maintained offer the opportunity to discover genes involved in several mutants of biomedical significance including two movement disorders. Peromyscus in the wild is a reservoir for two emerging infectious microorganisms.

描述（由申请人提供）：Peromyscus遗传储备中心（PGSC）提供了可靠的无疾病peromyscines的来源，并且对于可用的多种peromyscus物种和突变体而言是无与伦比的。 PGSC为各种领域提供动物，包括人类传染病，行为研究，生理适应和遗传研究。两项发展应进一步增强对Peromyscus作为实验生物的兴趣。 NHGRI根据我们提交的白皮书选择了一个6倍的序列，由3种物种组成的6倍序列和2x序列。这个项目正刚刚在贝勒医学院开始。与Glenn博士（乔治亚州）合作，向JGI/DOE提出了要求进行50,000个Peromyscus EST克隆的构建和测序。已经进行了EST克隆，并完成了质量评估。此完整的序列数据应在大约一个月内提供。此外，第一代链接图揭示了Peromysus和Rattus之间的联系比Peromyscus和Mus更紧密的联系。进一步的连锁和染色体绘画分析允许构建基于染色体的连锁图。这些资源将增强peromyscus作为重复运动障碍，少年和成人共济失调领域的基因发现领域的研究工具的重要性，以及癫痫，疾病和环境毒理学中的基因表达以及基因组知识的机制，Peromyscus是一种，Peromyscus是一种两种新兴传染性微生物的水库。 Hanta病毒会导致40％死亡的疾病，并且是潜在的生物恐怖剂。莱姆病的病原体也在Peromyscus中含有，每年发生近15,000例。 PGSC为研究这些疾病和其他疾病（例如ehrllichiosis，babesiosis and Plaque）提供了动物。该项目的研究方面是继续开发胚胎冷冻保存方法和重新推导活动物，以保留稀有突变体和种质，以便在需要时使用并降低成本。进步是使用小鼠中使用的激素治疗方案的修饰获得胚胎产生的增加。胚胎已成功冷冻并解冻为细胞分裂。未来的工作将进一步完善这些方法，目的是从冷冻胚胎中重新衍生活动物。 公共卫生相关性（由申请人提供）：作为一种物种，Peromyscus自然发生在不同的栖息地中，提供独特的模型来研究与适应特定环境有关的遗传变异。自然存在的突变体被维持，这提供了发现涉及几种具有生物医学意义突变体的基因，包括两种运动障碍。野外的peromyscus是两种新兴传染性微生物的储层。