Development of Peromyscus Genomics

白鼠基因组学的发展

• 批准号: 7269363
• 负责人: Michael Ray Felder
• 金额: $ 30.41万
• 依托单位: UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7269363
• 关键词: American; Attention; Back; Behavior; Behavioral; Biological Assay; Biomedical Research; Candidate Disease Gene; Cell Line; Cells; Chinese Hamster; Chromosome Mapping; Code; Communicable Diseases; Complement; Condition; Conscious; Deer Mouse; Development; Development, Other; Dissection; Drosophila genus; Emerging Communicable Diseases; Facility Construction Funding Category; Figs - dietary; Generic Drugs; Genes; Genetic; Genetic Markers; Genetic Polymorphism; Genome; Genomics; Goals; Habitats; House mice; Hybrids; Individual; Lung; Lyme Disease; Mammals; Maps; Meiosis; Microbe; Microsatellite Repeats; Molecular; Mus; Numbers; Parasites; Parents; Peromyscus; Physiological; Polymerase Chain Reaction; Proteins; Quantitative Trait Loci; Radiation Hybrid; Research; Research Personnel; Resources; Rosa; Series; Sister; Study models; Syndrome; Synteny; base; density; environmental change; genetic linkage analysis; genetic manipulation; improved; microbial; reproductive; size; tool; trait

DESCRIPTION (provided by applicant): Peromyscines are the most widely distributed and abundant native North American mammals. Recently Peromyscus leucopus and P maniculatus abruptly rose to national attention when they were discovered to be the primary reservoirs of microbes causing two emerging infectious diseases: Lyme disease and Hantaviral pulmonary syndrome. Aptly called "The Drosophila of North American Mammology" peromyscines are also considered to be an ideal model for studying 1) the genes responsible for reproductive isolation and speciation, and 2) the genes enabling the physiological and behavioral adaptation to changing environmental conditions, adaptation to other species, adaptation to each other, and adaptation to microbial and other parasites. An important tool for exploiting this potential is a linkage map. It is the Aim of this proposal to develop an intermediate-density linkage map of P maniculatus bairdii using PCR based markers at sufficient density, approximately 5-10 cM, to permit identification of major segments syntenic with the reference species, Mus musculus. Such markers consist of 1) Type I (Protein Coding Genes) important for synteny identification, and 2) Type II (Microsatellites), which are highly polymorphic and will ultimately be used for QTL analysis. The linkage analysis panel was selected for maximizing utilizable polymorphism and is composed of Interspecific Meiotic Segregants of crosses between the sister species P m bairdii and P polionotus. Development of a Peromyscus linkage map will markedly improve the research potential of the species, enhancing projects currently underway and encouraging the development of other projects that explore the genetics of host-parasite interactions, speciation, behavior and the various aspects of adaptive trait acquisition. Specific information enabled by such a map includes QTL analysis, genetic manipulation and dissection of individual QTLs, and identification of potential candidate genes for individual QTLs.

描述（由申请人提供）：Peromyscines 是分布最广、数量最多的北美本土哺乳动物。最近，白足鼠（Peromyscus leucopus）和足足鼠（Pomyscus maniculatus）被发现是引起两种新发传染病（莱姆病和汉坦病毒肺综合征）的微生物的主要储存宿主，从而突然引起全国关注。被恰如其分地称为“北美哺乳动物学果蝇”的peromyscines也被认为是研究1）负责生殖隔离和物种形成的基因，以及2）使生理和行为适应不断变化的环境条件的基因的理想模型。其他物种、相互适应以及对微生物和其他寄生虫的适应。 开发这种潜力的一个重要工具是链接图。本提案的目的是使用基于 PCR 的标记以足够的密度（约 5-10 cM）开发 P maniculatus bairdii 的中等密度连锁图谱，以允许鉴定与参考种 Mus musculus 同线的主要片段。此类标记包括 1) 对于同线性鉴定很重要的 I 型（蛋白质编码基因）和 2) II 型（微卫星），它们具有高度多态性，最终将用于 QTL 分析。选择连锁分析组以最大化可利用的多态性，并且由姐妹种 P m bairdii 和 P poliionotus 之间杂交的种间减数分裂分离体组成。 Peromyscus连锁图谱的开发将显着提高该物种的研究潜力，增强目前正在进行的项目，并鼓励其他项目的开发，这些项目探索宿主-寄生虫相互作用的遗传学、物种形成、行为以及适应性特征获取的各个方面。这种图谱提供的具体信息包括 QTL 分析、个体 QTL 的遗传操作和解剖，以及个体 QTL 的潜在候选基因的鉴定。

项目成果

A genetic map of Peromyscus with chromosomal assignment of linkage groups (a Peromyscus genetic map).

• DOI: 10.1007/s00335-014-9500-8
• 发表时间: 2014-04
• 期刊: MAMMALIAN GENOME
• 影响因子: 2.5
• 作者: Kenney-Hunt, Jane;Lewandowski, Adrienne;Glenn, Travis C.;Glenn, Julie L.;Tsyusko, Olga V.;O'Neill, Rachel J.;Brown, Judy;Ramsdell, Clifton M.;Quang Nguyen;Phan, Tony;Shorter, Kimberly R.;Dewey, Michael J.;Szalai, Gabor;Vrana, Paul B.;Felder, Michael R.
• 通讯作者: Felder, Michael R.