Genotyping and Microarray Core

基因分型和微阵列核心

基本信息

批准号：
8001196
负责人：
RICHARD C DAVIS
金额：
$ 27.74万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-07-01 至 2015-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8001196
关键词：
Animal Model Arts Automation Biological Assay Biostatistics Core Blood capillaries California Candidate Disease Gene Chromosome Mapping Clinic Complex Computer software Computers Coronary Arteriosclerosis Custom DNA DNA Sequence Analysis Data Data Files Detection Development Disease Dyes Electrophoresis Equipment Family Fluorescence Fluorescent Dyes Gene Chips Gene Expression Genes Genetic Genetic Polymorphism Genetic Transcription Genetic screening method Genome Genotype House mice Human Human Gene Mapping Human Genetics Human Resources Inbred Strain Individual Investigation Label Laboratories Laboratory mice Liquid substance Maps Messenger RNA Metabolic syndrome Methods Microsatellite Repeats Molecular Profiling Mus Output Phenotype Polymerase Chain Reaction Polymorphism Analysis Population Preparation Process Publishing Radiolabeled Rattus Reaction Reader Reading Reagent Reliance Research Personnel Resources Robotics Role Scanning Screening procedure Sequence Analysis Services Short Tandem Repeat Single Nucleotide Polymorphism Solutions Surveys System Techniques Technology Testing Work capillary cost experience genetic linkage genome-wide high throughput analysis high throughput technology improved interest offspring programs radiotracer research facility trait

项目摘要

DESCRIPTION OF CORE UNIT 1. Overview The role for this core is to support all the projects in their requirements for genome-wide expression analysis, single nucleotide polymorphism (SNP)^ analysis, gene mapping and sequencing. Support will be provided by several means with emphasis placed on: 1) Expression analysis by micro-array, 2) high volume scoring of SNP and, in some cases, microsatellite polymorphisms in humans and mice; and 3) the processing and analysis of dideoxy-terminated sequencing reactions produced by the individual projects. In addition, the core will aid the projects in the preparation of expression, genotype and sequence data in formats most useful for further analysis. These data will be formatted for forwarding to the Biostatistics Core (Core C). For followup investigation of individual genes, the core will assist the projects in qPCR and analysis by R-QTL. State-of-the-art genotyping and expression analysis, with its reliance specialized equipment and partial automation, is most efficiently carried out in a core laboratory that is staffed by experienced personnel and that is specifically organized for this work. The centralization of genotyping in Core B will both increase efficiency and decrease supply costs for the Program Project by allowing high throughput technologies and allowing bulk purchases of reagents.

核心单元说明 1. 概述该核心的作用是支持所有项目的全基因组表达需求分析、单核苷酸多态性 (SNP)^ 分析、基因作图和测序。将通过多种方式提供支持，重点是：1）通过微阵列进行表达分析，2）SNP 的大量评分，以及在某些情况下，人类和小鼠中的微卫星多态性； 3) 各个项目产生的双脱氧终止测序反应的处理和分析。此外，该核心将帮助项目准备表达、基因型和序列数据，其格式对进一步分析最有用。这些数据将被格式化以转发到生物统计核心（核心 C）。对于单个基因的后续研究，核心将协助qPCR和R-QTL分析项目。最先进的基因分型和表达分析依靠专业设备和部分自动化，在核心实验室中最有效地进行，该实验室由经验丰富的人员组成，并且是专门为这项工作而组织的。通过允许高通量技术和允许批量购买试剂，核心 B 中基因分型的集中化将提高效率并降低计划项目的供应成本。