Biolmaging Core

生物成像核心

基本信息

批准号：
7496323
负责人：
MICHAEL Douglas BOSKA
金额：
$ 10.58万
依托单位：
UNIVERSITY OF NEBRASKA MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-05-15 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7496323
关键词：
AIDS Dementia Complex AIDS neuropathy Advanced Development Animal Model Animals Arts Automation Biochemical Biodistribution Biological Markers Blood - brain barrier anatomy Brain Cells Chronic Complement Core Facility Count Custom Data Data Analyses Detection Development Developmental Therapeutics Program Diagnosis Disease Disease Progression Disease model Dissection Facility Construction Funding Category Germany Goals Histology Histopathology Human Image Impaired cognition Invasive Investigational Therapies Iron Kinetics Label Laboratories Magnetic Resonance Imaging Maps Measures Medical center Metabolic Methods Modality Modification Molecular Probes Monitor Mus Nebraska Nerve Degeneration Nervous system structure Neurodegenerative Disorders Neurons Neurophysiology - biologic function Neurovirology Noise Perfusion Permeability Photons Physiological Positron-Emission Tomography Process Protons Prussian blue Radioisotopes Range Research Resolution Rodent Rodent Model Sampling Signal Transduction Slice Slide Source Spectrum Analysis Spin Labels Staining method Stains Standards of Weights and Measures Support of Research System Technology Therapeutic Therapeutic Intervention Tissue Sample Tissues Universities Validation Work bioimaging cell motility design design and construction human disease image processing image warping improved iron oxide macrophage magnetic resonance spectroscopic imaging migration mouse model nerve stem cell neuroimaging neuropathology novel programs response single photon emission computed tomography software development spectroscopic imaging validation studies

项目摘要

This core will provide state-of-the-art bioimaging support for projects 1, 2, and 3, J. Zheng, H. Gendelman, and Y. Persidsky. The work will include quantitative magnetic resonance imaging (MRI), MR spectroscopic imaging (MRSI), and single photon emission computed tomography (SPECT). Two 7-Tesla small animal MRI/S systems, now operative at the University of Nebraska Medical Center, will provide quantitative neuroimaging and superparamagnetic iron oxide (SPIO) labeled cell tracking for rodent neuroAIDS animal models of HIV-1 associated dementia (HAD). One GammaMedica Ideas animal SPECT will be used in determining biodistribution of gamma emitter labeled cells or molecular probes and provides an excellent complement to MRI cell tracking methods. The image-processing laboratory has developed custom MRI/SPECT animal holders incorporating visible fiducial markers to allow coregistration of these two modalities. This allows anatomical details for SPECT images and validation of cell quantification by MRI. The bioimaging core methods also include quantitative mapping of blood-brain barrier permeability (project 3, Y. Persidsky), quantitative arterial spin labeled perfusion mapping, and quantitative proton MRSI (1H MRSI) (projects 2 and 3, H. Gendelman and Y. Persidsky). In addition, advanced imaging and spectroscopic analysis methods have been developed within the MRI/S core facility to allow automated mouse brain subimaging and coregistration/warping of imaging results with histology (all projects). This core will also support developmental therapeutics relevant to microglial activation in HAD. The long-term aims of these works are to assess the physiological MRI and biochemical 1H MRSI predictors of neuropathology and cell migration dynamics (MRI and SPECT) in mouse models of neuroAIDS. This will provide sensitive and specific screens of the onset and/or progression of disease as well as providing response kinetics to a variety of therapeutic interventions. The results obtained from this core will have direct applicability for monitoring the course of human neurodegenerative disorders. Significant modifications of the core were made in both focus and design so that it can unambiguously provide novel data that cannot readily be btained by histopathological examinations alone.

该核心将为项目 1、2 和 3 提供最先进的生物成像支持，J. Cheng、H. Gendelman、和 Y. Persidsky。这项工作将包括定量磁共振成像（MRI）、磁共振波谱成像（MRSI）和单光子发射计算机断层扫描（SPECT）。两只7特斯拉小动物 MRI/S 系统现已在内布拉斯加大学医学中心运行，将提供定量啮齿动物神经艾滋病动物的神经成像和超顺磁性氧化铁（SPIO）标记细胞追踪 HIV-1相关痴呆（HAD）模型。一种 GammaMedica Ideas 动物 SPECT 将用于确定伽马发射器标记的细胞或分子探针的生物分布，并提供了极好的 MRI 细胞追踪方法的补充。图像处理实验室开发了定制的 MRI/SPECT 动物支架包含可见的基准标记，以允许这两者共同配准方式。这样可以获取 SPECT 图像的解剖细节并通过 MRI 验证细胞量化。生物成像核心方法还包括血脑屏障渗透性的定量绘图（项目 3，Y. Persidsky），定量动脉自旋标记灌注图和定量质子 MRSI（1H MRSI）（项目 2 和 3，H. Gendelman 和 Y. Persidsky）。此外，先进的成像和光谱 MRI/S 核心设施内已开发出分析方法，可实现小鼠大脑的自动化组织学成像结果的子成像和共同配准/变形（所有项目）。该核心还将支持与 HAD 中小胶质细胞激活相关的发育治疗。这些的长期目标工作是评估神经病理学和细胞的生理 MRI 和生化 1H MRSI 预测因子神经艾滋病小鼠模型中的迁移动力学（MRI 和 SPECT）。这将提供敏感和疾病发作和/或进展的具体筛查以及提供对疾病的反应动力学各种治疗干预措施。从该核心获得的结果将直接适用于监测人类神经退行性疾病的进程。核心的重大修改是在焦点和设计上都进行了制作，以便它可以明确地提供不容易被识别的新颖数据仅通过组织病理学检查获得。